Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,883,644	A→G	T153A (ACC→GCC)	yeaV →	putative transporter YeaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,883,644	0	A	G	100.0%	155.4 / NA	44	T153A (ACC→GCC)	yeaV	putative transporter YeaV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/24);  total (20/24)

AAACAAAGGTCTGAGCCTTTCCGGCATTATTGCTGCTATTACCGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTACCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGATTGGTATCAACAACGGTTTGCA  >  NC_000913/1883511‑1883780                                                                                                                                      |                                                                                                                                         aaaCAAAGGTCTGAGCCTTTCCGGCATTATTGCTGCTATTACCGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCgcag                                                                                                                                    <  1:80602/140‑1 (MQ=255) aaaCAAAGGTCTGAGCCTTTCCGGCATTATTGCTGCTATTACCGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCgcag                                                                                                                                    <  1:58642/140‑1 (MQ=255)                   ttCCGGCATTATTGCTGCTATTACCGTCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGc                                                                                                                  >  2:183354/1‑140 (MQ=255)                              ttGCTGCTATTACCGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCcg                                                                                                            >  1:30486/1‑135 (MQ=255)                              ttGCTGCTATTACCGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCcg                                                                                                            <  2:30486/135‑1 (MQ=255)                                      aTTACCGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGttt                                                                                               >  2:276686/1‑140 (MQ=255)                                      aTTACCGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGttt                                                                                               >  2:786745/1‑140 (MQ=255)                                         aCCGGCGTTCGCCCGCAAGGCCCATGGGGAGAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTAcc                                                                                            >  2:76621/1‑140 (MQ=255)                                         aCCGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGa                                                                                                      >  1:908534/1‑130 (MQ=255)                                         aCCGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGa                                                                                                      <  2:908534/130‑1 (MQ=255)                                         aCCGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTAcc                                                                                            <  2:508039/140‑1 (MQ=255)                                          ccGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTAccc                                                                                           <  2:441948/140‑1 (MQ=255)                                            ggCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGCTGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGa                                                                                         <  2:1010904/140‑1 (MQ=255)                                              cGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTAccc                                                                                           >  2:463721/1‑136 (MQ=255)                                              cGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTAccc                                                                                           <  1:463721/136‑1 (MQ=255)                                              cGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATa                                                                                       >  2:897070/1‑140 (MQ=255)                                              cGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATa                                                                                       >  1:256608/1‑140 (MQ=255)                                              cGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATa                                                                                       <  1:209601/140‑1 (MQ=255)                                                    cccGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCa                                                                                 <  2:1017625/140‑1 (MQ=255)                                                        cAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGt                                                                             <  1:827958/140‑1 (MQ=255)                                                         aaGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTg                                                                            <  1:249080/140‑1 (MQ=255)                                                          aGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGc                                                                           <  1:935867/140‑1 (MQ=255)                                                          aGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGc                                                                           <  1:183354/140‑1 (MQ=255)                                                           ggCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCa                                                                          <  1:897070/140‑1 (MQ=255)                                                                       aaaCTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAgg                                                                        <  2:656721/130‑1 (MQ=255)                                                                       aaaCTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAgg                                                                        >  1:656721/1‑130 (MQ=255)                                                                            ggTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGc                                                         >  1:932410/1‑140 (MQ=255)                                                                              tCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCtt                                                       <  2:570759/140‑1 (MQ=255)                                                                                                   ccACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTa                                  >  2:618079/1‑140 (MQ=255)                                                                                                    cACTGTCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTaa                                 >  1:545394/1‑140 (MQ=255)                                                                                                         tCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCt                                   <  1:768505/133‑1 (MQ=255)                                                                                                         tCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCt                                   >  2:768505/1‑133 (MQ=255)                                                                                                         tCGGCGCACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCg                            >  2:487453/1‑140 (MQ=255)                                                                                                             cgcACTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGa                        <  1:863841/140‑1 (MQ=255)                                                                                                                aCTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGATTg                     >  1:344949/1‑140 (MQ=255)                                                                                                                 cTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGATTg                     >  2:4543/1‑139 (MQ=255)                                                                                                                 cTGACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGATTg                     <  1:4543/139‑1 (MQ=255)                                                                                                                   gACCATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGATTGGTa                  >  2:940655/1‑140 (MQ=255)                                                                                                                     ccATTTCCCTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGATTGGTATc                <  2:932410/140‑1 (MQ=255)                                                                                                                         ttCCCTTGTTGTTGCCACAGCAACCTTTACCCGTGGGCTTTCCGCGCTGATCGGTTTACCAGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGATTGGTATcaaca            <  2:971559/140‑1 (MQ=255)                                                                                                                            ccTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGATTGGTATCAACAACg         >  1:281021/1‑140 (MQ=255)                                                                                                                            ccTTGTTGTTGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCA‑TTTTTGCCTAAGCTCGTGGATTGGTATCAACAACgg        <  1:76621/140‑1 (MQ=255)                                                                                                                                  tgttGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGATTGGTATCAACAACGGTTTGc   <  2:344949/140‑1 (MQ=255)                                                                                                                                   gttGCCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGATTGGTATCAACAACGGTTTGCa  >  2:77813/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         AAACAAAGGTCTGAGCCTTTCCGGCATTATTGCTGCTATTACCGGCGTTCGCCCGCAAGGCCCATGGGGAAAACTGGTCGATTTGATGTTCCTGATCGCCACTGTCGGCGCACTGACCATTTCCCTTGTTGTTACCGCAGCAACCTTTACCCGTGGGCTTTCCGCGCTGACCGGTTTACCCGATAACTTCACCGTGCAGGCATTTGTGATCCTGCTTTCCGGCGGCATTTTTTGCCTAAGCTCGTGGATTGGTATCAACAACGGTTTGCA  >  NC_000913/1883511‑1883780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑