Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,893,022	A→G	S72P (TCA→CCA)	yoaA ←	putative 5' to 3' DNA helicase implicated in DNA repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,893,022	0	A	G	100.0%	145.2 / NA	42	S72P (TCA→CCA)	yoaA	putative 5' to 3' DNA helicase implicated in DNA repair
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/18);  total (24/18)

CGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTAC  >  NC_000913/1892888‑1893151                                                                                                                                       |                                                                                                                                  cgcCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCgg                                                                                                                              >  2:18292/1‑140 (MQ=255)  gcCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGt                                                                                                                             <  1:62977/140‑1 (MQ=255)  gcCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGt                                                                                                                             <  2:541520/140‑1 (MQ=255)                    tCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGActttctt                                                                                                           >  2:690960/1‑140 (MQ=255)                              ggTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCcgc                                                                                                 >  2:859122/1‑140 (MQ=255)                                  gTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCcgcagcg                                                                                             >  2:58383/1‑140 (MQ=255)                                    ttGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCcgcagcgca                                                                                           <  1:254927/140‑1 (MQ=255)                                    ttGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCcgcagcgca                                                                                           <  1:18292/140‑1 (MQ=255)                                       aGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCcgcagcgca                                                                                           >  2:641660/1‑137 (MQ=255)                                       aGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCcgcagcgca                                                                                           <  1:641660/137‑1 (MQ=255)                                       aGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGa                                                                                        >  1:538569/1‑140 (MQ=255)                                                      gcgcCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGGTTTTGGGCCGGTCGAGATAATGACTTTCTTGTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAg                                                                         >  2:863724/1‑140 (MQ=255)                                                      gcgcCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGt                                                                                 <  2:719875/132‑1 (MQ=255)                                                      gcgcCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGt                                                                                 >  1:719875/1‑132 (MQ=255)                                                      gcgcCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATAACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAg                                                                         >  1:244343/1‑140 (MQ=255)                                                       cgcCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAgg                                                                        >  2:444859/1‑140 (MQ=255)                                                         ccACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGtt                                                                      <  1:868146/140‑1 (MQ=255)                                                             gTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGc                                                                  <  2:866298/140‑1 (MQ=255)                                                                 cccGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGt                                                              >  1:158969/1‑140 (MQ=255)                                                                     tataTTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGcc                                                                 >  1:71646/1‑133 (MQ=255)                                                                     tataTTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGcc                                                                 <  2:71646/133‑1 (MQ=255)                                                                        atTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGtt                                                       <  1:58383/140‑1 (MQ=255)                                                                         tttCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTc                                                      >  1:152878/1‑140 (MQ=255)                                                                         tttCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTc                                                      >  1:385167/1‑140 (MQ=255)                                                                            cAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTg                                                   <  1:181172/140‑1 (MQ=255)                                                                              aTGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCt                                                 <  2:385167/140‑1 (MQ=255)                                                                               tGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAATCGTAGGTTTTGCCCGTACCGGTTCCTGCtt                                                >  2:757605/1‑140 (MQ=255)                                                                                  cTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTcca                                             <  1:444859/140‑1 (MQ=255)                                                                                    ttGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTccacc                                           >  1:77502/1‑140 (MQ=255)                                                                                       agaCTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTccaccacc                                        <  1:859122/140‑1 (MQ=255)                                                                                           tGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCt                                                    <  2:338497/124‑1 (MQ=255)                                                                                           tGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCt                                                    >  1:338497/1‑124 (MQ=255)                                                                                           tGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCg                                    >  1:1060145/1‑140 (MQ=255)                                                                                            gTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCgg                                   >  1:680091/1‑140 (MQ=255)                                                                                            gTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCgg                                   >  2:80589/1‑140 (MQ=255)                                                                                                       gcgGCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCttttt                        >  2:6529/1‑140 (MQ=255)                                                                                                          gCTGTAGAGCTGATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTa                     >  2:56271/1‑140 (MQ=255)                                                                                                                     gATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTg          <  1:228150/140‑1 (MQ=255)                                                                                                                     gATCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTg          <  1:730421/140‑1 (MQ=255)                                                                                                                      aTCCTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGa         >  1:604519/1‑140 (MQ=255)                                                                                                                         cTGCAACGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACgg      <  2:660916/140‑1 (MQ=255)                                                                                                                             aaCGCTTTTGGGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTAc  >  2:139388/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                  CGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCAGCGCCACGTTGCCCGTATATTTCAATGCCTTTGAGACTGTTGGCAAATCGCGGCTGTAGAGCTGATCCTGCAACGCTTTTGAGCCGGTCGAGATAATGACTTTCTTTTTCGCCCGCAGCGCAGGAGCCAGGTAAGCGTAGGTTTTGCCCGTACCGGTTCCTGCTTCCACCACCAGCGGCTGGCCTTTTTCTATCGCCTGGGTGACGGCTAC  >  NC_000913/1892888‑1893151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑