Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,900,304	T→C	T428A (ACG→GCG)	yoaE ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,900,304	0	T	C	100.0%	178.0 / NA	50	T428A (ACG→GCG)	yoaE	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/34);  total (16/34)

TCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGT  >  NC_000913/1900172‑1900438                                                                                                                                     |                                                                                                                                       tCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCa                                                                                                                                 <  2:640126/140‑1 (MQ=255)    cGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCg                                                                                                                              <  2:786931/140‑1 (MQ=255)         cccGCGACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATACAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCgg                                                                                                                         >  1:460554/1‑140 (MQ=255)           cgcgACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCa                                                                                                                                 <  2:327266/130‑1 (MQ=255)           cgcgACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCa                                                                                                                                 >  1:327266/1‑130 (MQ=255)                 cGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCAc                                                                                                                 <  1:326409/140‑1 (MQ=255)                  gTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCAcc                                                                                                                <  1:1049326/140‑1 (MQ=255)                   tCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGATTTCAccc                                                                                                               >  2:1061336/1‑140 (MQ=255)                      cgATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCa                                                                                                            <  2:357145/140‑1 (MQ=255)                      cgATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCa                                                                                                            <  1:607825/140‑1 (MQ=255)                      cgATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCa                                                                                                            <  1:900943/140‑1 (MQ=255)                        atatCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAAt                                                                                                          <  2:108877/140‑1 (MQ=255)                             atcgtcatcgGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCtt                                                                                                     <  1:294541/140‑1 (MQ=255)                             atcgtcatcgGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATACAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCtt                                                                                                     <  2:460554/140‑1 (MQ=255)                                   atcgGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGca                                                                                               >  2:332382/1‑140 (MQ=255)                                     cgGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGcaca                                                                                             >  1:652061/1‑140 (MQ=255)                                         aaGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCa                                                                                         <  2:432882/140‑1 (MQ=255)                                         aaGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCa                                                                                         <  1:192797/140‑1 (MQ=255)                                           ggTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATg                                                                                                         <  1:153162/122‑1 (MQ=255)                                           ggTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATg                                                                                                         >  2:153162/1‑122 (MQ=255)                                              gCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAgcggc                                                                                    <  2:447494/140‑1 (MQ=255)                                              gCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAgcggc                                                                                    <  2:748869/140‑1 (MQ=255)                                                    cATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGAcc                                                                              <  2:509800/140‑1 (MQ=255)                                                    cATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGAcc                                                                              <  2:157752/140‑1 (MQ=255)                                                    cATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGAcc                                                                              <  2:453443/140‑1 (MQ=255)                                                    cATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGAcc                                                                              <  2:652061/140‑1 (MQ=255)                                                    cATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGAcc                                                                              <  1:458900/140‑1 (MQ=255)                                                     aTCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCCCCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCa                                                                             <  1:746324/140‑1 (MQ=255)                                                     aTCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCa                                                                             <  1:209088/140‑1 (MQ=255)                                                       cAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGAcc                                                                              <  2:118178/137‑1 (MQ=255)                                                       cAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGAcc                                                                              >  1:118178/1‑137 (MQ=255)                                                         aGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGAcc                                                                         <  2:1076813/140‑1 (MQ=255)                                                               tgctgcAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGAcc                                                                              <  2:333614/129‑1 (MQ=255)                                                               tgctgcAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGAcc                                                                              >  1:333614/1‑129 (MQ=255)                                                               tgctgcAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTAc                                                                   <  1:802841/140‑1 (MQ=255)                                                               tgctgcAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTAc                                                                   >  2:383161/1‑140 (MQ=255)                                                                      aGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCa                                                            >  1:773344/1‑140 (MQ=255)                                                                           tGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTc                                                       >  2:711660/1‑140 (MQ=255)                                                                            gCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCg                                                      <  1:761285/140‑1 (MQ=255)                                                                                 tCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGt                                                 <  2:434539/140‑1 (MQ=255)                                                                                 tCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGt                                                 <  1:332382/140‑1 (MQ=255)                                                                                 tCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGt                                                 <  2:773344/140‑1 (MQ=255)                                                                                   tGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCa                                               <  2:1018411/140‑1 (MQ=255)                                                                                               tACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGc                                   >  1:366290/1‑140 (MQ=255)                                                                                                          cGTCACCATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCa                        <  1:933226/140‑1 (MQ=255)                                                                                                               ccATCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCacgacg                   >  1:1034278/1‑140 (MQ=255)                                                                                                                 aTCAGTAATGATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACgca                 <  1:995667/140‑1 (MQ=255)                                                                                                                          gATTTCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCa        >  1:676341/1‑140 (MQ=255)                                                                                                                              tCCGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAg    >  1:548532/1‑140 (MQ=255)                                                                                                                                cGGCGCTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACGACACCAAACTCGTTGGTCACGATAACAAAGATCCCGCGAGCACGACGCAGCACGCCCAACAGGt  >  2:864813/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       TCACGAGGCCCGCGACCGTCGCGATATCATCGTCATCGGCAAGGTGCTCAACATCAAGCGCCTGCTGCAAGGCATGCAAATCTGTACCGCCTTTTACCAGCCAGCCGTCACCATCAGTAATGATTTCCGGCGTTTCGTCAGCGTCCGGGAATTCACCCGCAATGGCTTCCAGCACATCCAGCGGCGTGACCAGACCTTGTACCACACCAAACTCGTTGGTCACGATAACAAAGCTCCCGCGAGCACGACGCAGCACGCCCAACAGGT  >  NC_000913/1900172‑1900438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑