Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,918,762	T→C	V418A (GTA→GCA)	yebT →	intermembrane transport protein YebT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,918,762	0	T	C	100.0%	154.7 / NA	44	V418A (GTA→GCA)	yebT	intermembrane transport protein YebT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/21);  total (23/21)

CCACGCAAAGAGTTCGTTGTTGTGCCAGGCGAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGTAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGC  >  NC_000913/1918626‑1918896                                                                                                                                         |                                                                                                                                       ccACGCAAAGAGTTCGTTGTTGTGCCAGGCGAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGcaggcagg                                                                                                                                     >  2:312599/1‑140 (MQ=255) ccACGCAAAGAGTTCGTTGTTGTGCCAGGCGAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGcaggcagg                                                                                                                                     >  2:169995/1‑140 (MQ=255)          gagTTCGTTGTTGTGCCAGGCGAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTat                                                                                                                            <  2:465227/140‑1 (MQ=255)               cGTTGTTGTGCCAGGCGAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTatcgatc                                                                                                                       <  1:827575/140‑1 (MQ=255)                    ttgtGCCAGGCGAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGNTATCGATCGTaaa                                                                                                                  <  1:1033692/140‑1 (MQ=255)                            ggCGAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAg                                                                                                          >  2:210457/1‑140 (MQ=255)                              cGAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCa                                                                                                        >  2:295315/1‑140 (MQ=255)                              cGAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCa                                                                                                        <  1:740341/140‑1 (MQ=255)                              cGAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTAACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACAGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCa                                                                                                        >  2:1013571/1‑140 (MQ=255)                               gAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCaa                                                                                                       >  2:224748/1‑140 (MQ=255)                                     gCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGt                                                                                                 <  1:312599/140‑1 (MQ=255)                                     gCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGt                                                                                                 <  1:677640/140‑1 (MQ=255)                                          gctgcATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCt                                                                                            <  1:576661/140‑1 (MQ=255)                                                   aCCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCg                                                                                   >  2:621522/1‑140 (MQ=255)                                                       gATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCAt                                                                               <  1:224748/140‑1 (MQ=255)                                                       gATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCAt                                                                               <  2:1065291/140‑1 (MQ=255)                                                       gATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCAt                                                                               <  2:1014027/140‑1 (MQ=255)                                                         tGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCg                                                                             <  1:621522/140‑1 (MQ=255)                                                                            accGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACt                                                          >  1:608844/1‑140 (MQ=255)                                                                                cgcCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTa                                                      >  1:894567/1‑140 (MQ=255)                                                                                cgcCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTa                                                      >  1:878698/1‑140 (MQ=255)                                                                                  ccGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTaaa                                                    >  2:527434/1‑140 (MQ=255)                                                                                     gAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAgg                                                 <  1:230500/140‑1 (MQ=255)                                                                                                  ttGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAAttt                                    >  1:897261/1‑140 (MQ=255)                                                                                                  ttGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAAttt                                    >  1:62445/1‑140 (MQ=255)                                                                                                   tGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTg                                   <  1:791969/140‑1 (MQ=255)                                                                                                    gATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTgt                                  >  1:407805/1‑140 (MQ=255)                                                                                                       gCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTgtcgt                               <  2:656669/140‑1 (MQ=255)                                                                                                        cGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTgtcgtc                              >  2:434359/1‑140 (MQ=255)                                                                                                          ggTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCaa                            >  2:1083303/1‑140 (MQ=255)                                                                                                          ggTCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCaa                            >  2:66354/1‑140 (MQ=255)                                                                                                            tCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCaa                            >  1:453730/1‑138 (MQ=255)                                                                                                            tCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCaa                            <  2:453730/138‑1 (MQ=255)                                                                                                            tCAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCCTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGAAAGCAAAATTGGCGTCAACa                          >  2:53212/1‑140 (MQ=255)                                                                                                             cAGCCGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAg                         <  1:210457/140‑1 (MQ=255)                                                                                                                ccGCTCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCg                      >  1:964193/1‑140 (MQ=255)                                                                                                                    tCATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTc                  <  1:1083303/140‑1 (MQ=255)                                                                                                                     cATTCTTCACGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCg                 >  2:525732/1‑140 (MQ=255)                                                                                                                              cGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAgg        >  2:874444/1‑140 (MQ=255)                                                                                                                              cGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAgg        <  2:894567/140‑1 (MQ=255)                                                                                                                              cGGCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAgg        <  1:66354/140‑1 (MQ=255)                                                                                                                                gCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAgg        >  2:40428/1‑138 (MQ=255)                                                                                                                                gCGTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAgg        <  1:40428/138‑1 (MQ=255)                                                                                                                                  gTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAgg        >  2:245325/1‑136 (MQ=255)                                                                                                                                  gTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAgg        <  1:245325/136‑1 (MQ=255)                                                                                                                                  gTGCAGGCAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTggg    >  2:1022590/1‑140 (MQ=255)                                                                                                                                    gcaggcagGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGc  >  1:973551/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CCACGCAAAGAGTTCGTTGTTGTGCCAGGCGAAAAAGCACTGCTGCATGAACCTGATGTTCTGACGCTGACCCTGACCGCGCCGGAAAGTTACGGTATTGATGCGGGTCAGCCGCTCATTCTTCACGGCGTGCAGGTAGGCCAGGTTATCGATCGTAAACTCACCAGCAAAGGCGTCACCTTTACCGTCGCCATCGAGCCTCAGCATCGCGAACTGGTAAAAGGCGATAGCAAATTTGTCGTCAACAGCCGTGTCGATGTGAAGGTGGGGC  >  NC_000913/1918626‑1918896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑