Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,975,369	T→C	Q296R (CAG→CGG)	motB ←	motility protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,975,369	0	T	C	100.0%	200.8 / NA	56	Q296R (CAG→CGG)	motB	motility protein B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/28);  total (28/28)

AAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTC  >  NC_000913/1975236‑1975504                                                                                                                                      |                                                                                                                                        aaaaCCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCgtggtg                                                                                                                                   <  1:701974/140‑1 (MQ=255)              gctgctCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGttt                                                                                                                      >  2:235376/1‑140 (MQ=255)                 gctCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTc                                                                                                                   >  1:864404/1‑140 (MQ=255)                 gctCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTc                                                                                                                   <  1:833921/140‑1 (MQ=255)                  ctCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTcc                                                                                                                  <  2:396436/140‑1 (MQ=255)                     cATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAgg                                                                                                               <  1:462799/140‑1 (MQ=255)                      aTGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAggg                                                                                                              <  2:113301/140‑1 (MQ=255)                       tGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGgc                                                                                                             >  2:294523/1‑140 (MQ=255)                        gTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGgcg                                                                                                            <  1:294523/140‑1 (MQ=255)                        gTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGgcg                                                                                                            >  1:992930/1‑140 (MQ=255)                                aCAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTgg                                                                                                    <  2:992930/140‑1 (MQ=255)                                aCAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTgg                                                                                                    <  2:712062/140‑1 (MQ=255)                                 cAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAgg                                                                                                               >  2:828953/1‑128 (MQ=255)                                 cAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAgg                                                                                                               <  1:828953/128‑1 (MQ=255)                                 cAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGc                                                                                                   <  2:244164/140‑1 (MQ=255)                                 cAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGc                                                                                                   >  1:218542/1‑140 (MQ=255)                                  aGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCt                                                                                                  <  1:235376/140‑1 (MQ=255)                                     tCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGc                                                                                                   <  2:872505/136‑1 (MQ=255)                                     tCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGc                                                                                                   >  1:816901/1‑136 (MQ=255)                                     tCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGc                                                                                                   >  1:872505/1‑136 (MQ=255)                                     tCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGc                                                                                                   <  2:816901/136‑1 (MQ=255)                                      cGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCAtt                                                                                              >  1:833366/1‑140 (MQ=255)                                      cGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCAtc                                                                                              >  2:1032753/1‑139 (MQ=255)                                         ccGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTg                                                                                           >  1:117470/1‑140 (MQ=255)                                                atcaAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCg                                                                                    >  1:761789/1‑140 (MQ=255)                                                               aTAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCaaa                                                                     <  1:1032753/140‑1 (MQ=255)                                                                    aaTCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGc                                                                >  2:448720/1‑140 (MQ=255)                                                                         cTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGtt                                                           >  2:315467/1‑140 (MQ=255)                                                                            atatCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCgg                                                        <  1:110961/140‑1 (MQ=255)                                                                            atatCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCgg                                                        <  2:864404/140‑1 (MQ=255)                                                                            atatCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCgg                                                        <  1:745488/140‑1 (MQ=255)                                                                                  aTGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCttgtt                                                  >  1:375923/1‑140 (MQ=255)                                                                                   tGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCttgt                                                   <  1:822131/138‑1 (MQ=255)                                                                                   tGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCttgt                                                   >  2:822131/1‑138 (MQ=255)                                                                                    gCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCttgtttg                                                >  2:49316/1‑140 (MQ=255)                                                                                          gCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGt                                          >  2:347146/1‑140 (MQ=255)                                                                                           cTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTtgtttgtt                                              <  2:67979/135‑1 (MQ=255)                                                                                           cTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTtgtttgtt                                              >  1:67979/1‑135 (MQ=255)                                                                                               cACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACcag                                     <  1:905579/140‑1 (MQ=255)                                                                                                aCCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACcagc                                    >  2:426468/1‑140 (MQ=255)                                                                                                    cGGTTCGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGc                                >  2:592375/1‑140 (MQ=255)                                                                                                         cGGCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATg                           <  2:113517/140‑1 (MQ=255)                                                                                                          ggCTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGc                          >  2:30857/1‑140 (MQ=255)                                                                                                            cTGATGGCATTGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGa                        <  2:218542/140‑1 (MQ=255)                                                                                                                     ttGTGGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACg               <  1:448720/140‑1 (MQ=255)                                                                                                                      tgtgGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACg               <  1:782944/139‑1 (MQ=255)                                                                                                                      tgtgGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACg               >  2:782944/1‑139 (MQ=255)                                                                                                                       gtgGGAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGc             <  1:315467/140‑1 (MQ=255)                                                                                                                           gAACACTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGcatca         >  1:866448/1‑140 (MQ=255)                                                                                                                             acacTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCatcatc       <  2:833366/140‑1 (MQ=255)                                                                                                                              cacTGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCAtcatca      >  2:934140/1‑140 (MQ=255)                                                                                                                                 tGACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGt   >  1:78009/1‑140 (MQ=255)                                                                                                                                  gACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCatcatc       <  2:729315/135‑1 (MQ=255)                                                                                                                                  gACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCatcatc       >  1:729315/1‑135 (MQ=255)                                                                                                                                  gACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTc  >  2:527934/1‑140 (MQ=255)                                                                                                                                  gACCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTc  >  1:862871/1‑140 (MQ=255)                                                                                                                                   aCCCGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGTCGGCatcatc       <  2:98469/134‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        AAAACCAGCAAATGCTGCTCCATGTCAGCCAACAGTTCGTCCGCTTCATCAAAAAATGTCTGATAAAAATCGCTTATATCCATGCTCACGCTGTCACCTCGGTTCGGCTGATGGCATTGTGGGAACACTGACCTGTGGTGCAACCTCAGGTTTTTCCAGGGCGCTTACTGGCTCATTCTGGCTTTCGGCGTTTTCATGCAAAATGGCCTGTTCGGCTTGTTTGTTCAGTACCAGCAGGCTGATGCGACGGTTGACGGCATCATCAGGTC  >  NC_000913/1975236‑1975504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑