Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,055,493	T→C	Y145H (TAC→CAC)	amn →	AMP nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,055,493	0	T	C	100.0%	227.4 / NA	64	Y145H (TAC→CAC)	amn	AMP nucleosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/27);  total (37/27)

CTTACGTTGCTGTATCAGGATTATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTAT  >  NC_000913/2055361‑2055627                                                                                                                                     |                                                                                                                                       cTTACGTTGCTGTATCAGGATTATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTccc                                                                                                                                 <  1:770209/140‑1 (MQ=255) cTTACGTTGCTGTATCAGGATTATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTccc                                                                                                                                 <  1:946987/140‑1 (MQ=255) cTTACGTTGCTGTATCAGGATGATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTccc                                                                                                                                 >  2:770209/1‑140 (MQ=255)                  ggATTATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTccc                                                                                                                                 <  1:113615/123‑1 (MQ=255)                  ggATTATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTccc                                                                                                                                 >  2:113615/1‑123 (MQ=255)                  ggATTATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTccc                                                                                                                                 <  1:684010/123‑1 (MQ=255)                  ggATTATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTccc                                                                                                                                 >  2:684010/1‑123 (MQ=255)                  ggATTATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGgcgc                                                                                                                >  2:628802/1‑140 (MQ=255)                    aTTATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCaa                                                                                                              >  1:590194/1‑140 (MQ=255)                     ttATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCaaa                                                                                                             <  1:477268/140‑1 (MQ=255)                         ggTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTgg                                                                                                         >  1:363119/1‑140 (MQ=255)                         ggTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTgg                                                                                                         >  2:803601/1‑140 (MQ=255)                         ggTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTgg                                                                                                         >  1:822859/1‑140 (MQ=255)                            gcgcATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGa                                                                                                      <  2:590194/140‑1 (MQ=255)                            gcgcATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGa                                                                                                      <  2:822859/140‑1 (MQ=255)                             cgcATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTAGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGAt                                                                                                     >  1:229856/1‑140 (MQ=255)                              gcATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATg                                                                                                    <  1:631802/140‑1 (MQ=255)                               cATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGa                                                                                                   >  1:676785/1‑140 (MQ=255)                                  atCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAAc                                                                                                >  2:719448/1‑140 (MQ=255)                                   tctcAGTGCAACCCTCGCAGCATGAAATCCCTTATCCCTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACt                                                                                               <  2:363119/140‑1 (MQ=255)                                        gTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGa                                                                                          <  1:1058995/140‑1 (MQ=255)                                            aaCCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGc                                                                                      <  1:396142/140‑1 (MQ=255)                                              cccTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCAt                                                                                    >  2:542113/1‑140 (MQ=255)                                                   gcagcaTGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTatc                                                                               >  1:867041/1‑140 (MQ=255)                                                   gcagcaTGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTatc                                                                               >  1:592238/1‑140 (MQ=255)                                                         tGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCaa                                                                         >  1:850036/1‑140 (MQ=255)                                                                 cttatccttatGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATtc                                                                 >  2:704300/1‑140 (MQ=255)                                                                  ttatccttatGTCATCGAGGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATtct                                                                >  1:647865/1‑140 (MQ=255)                                                                   tatccttatGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATtctc                                                               >  1:28046/1‑140 (MQ=255)                                                                     tccttatGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTccc                                                             <  2:286099/140‑1 (MQ=255)                                                                          atGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTa                                                        >  1:683393/1‑140 (MQ=255)                                                                           tGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCaa                                                                         >  1:525876/1‑122 (MQ=255)                                                                           tGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCaa                                                                         <  2:525876/122‑1 (MQ=255)                                                                               aTCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCa                                                   >  1:963666/1‑140 (MQ=255)                                                                                tCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTa                                                        >  1:707786/1‑134 (MQ=255)                                                                                tCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTa                                                        <  2:707786/134‑1 (MQ=255)                                                                                tCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCAt                                                  >  1:12543/1‑140 (MQ=255)                                                                                tCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCAt                                                  >  1:112566/1‑140 (MQ=255)                                                                                   aTGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCAtttt                                               >  1:638326/1‑140 (MQ=255)                                                                                       ctctGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATg                                           >  1:624042/1‑140 (MQ=255)                                                                                       ctctGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATg                                           >  1:675014/1‑140 (MQ=255)                                                                                                 acacTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGt                                 >  1:256433/1‑140 (MQ=255)                                                                                                    cTTGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACTGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGa                              >  1:1044588/1‑140 (MQ=255)                                                                                                      tGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGAtt                            >  2:905900/1‑140 (MQ=255)                                                                                                      tGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGAtt                            >  2:359301/1‑140 (MQ=255)                                                                                                      tGATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGAtt                            >  2:756852/1‑140 (MQ=255)                                                                                                       gATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCcgcg                                  <  1:394533/133‑1 (MQ=255)                                                                                                       gATCGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCcgcg                                  >  2:394533/1‑133 (MQ=255)                                                                                                          cGCTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTc                        <  2:676785/140‑1 (MQ=255)                                                                                                            cTCAATGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTccc                      >  2:536568/1‑140 (MQ=255)                                                                                                                aTGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGc                  <  2:963666/140‑1 (MQ=255)                                                                                                                 tGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCa                 <  1:704300/140‑1 (MQ=255)                                                                                                                 tGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCa                 <  1:542113/140‑1 (MQ=255)                                                                                                                 tGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCa                 >  2:182093/1‑140 (MQ=255)                                                                                                                 tGAGCGCTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCa                 <  2:12543/140‑1 (MQ=255)                                                                                                                     cgcTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGt             >  1:1062459/1‑140 (MQ=255)                                                                                                                       cTGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTg           <  1:964181/140‑1 (MQ=255)                                                                                                                        tGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTgc          <  1:536568/140‑1 (MQ=255)                                                                                                                        tGGGTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTgc          <  1:359301/140‑1 (MQ=255)                                                                                                                         gggTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCa                 <  1:256003/132‑1 (MQ=255)                                                                                                                         gggTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCa                 >  2:256003/1‑132 (MQ=255)                                                                                                                          ggTTAACTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTgcgc        <  1:803601/140‑1 (MQ=255)                                                                                                                               aCTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTgc          >  1:893308/1‑133 (MQ=255)                                                                                                                               aCTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTgc          <  2:893308/133‑1 (MQ=255)                                                                                                                                cTCGCCACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATtat  <  2:683393/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       CTTACGTTGCTGTATCAGGATTATGGTGCGCATATCTCAGTGCAACCCTCGCAGCATGAAATCCCTTATCCTTATGTCATCGATGGCTCTGAATTGACACTTGATCGCTCAATGAGCGCTGGGTTAACTCGCTACTTCCCGACAACAGAACTGGCGCAAATTGGCGATGAAACTGCAGACGGCATTTATCATCCAACTGAATTCTCCCCGCTATCGCATTTTGATGCGCGCCGCGTCGATTTTTCCCTCGCACGGTTGCGCCATTAT  >  NC_000913/2055361‑2055627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑