Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,134,004 A→G V550A (GTG→GCG)  wzc ← protein‑tyrosine kinase Wzc

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,134,0040AG100.0% 173.4 / NA 49V550A (GTG→GCG) wzcprotein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/27);  total (22/27)

CGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATC  >  NC_000913/2133882‑2134135
                                                                                                                          |                                                                                                                                   
cGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGgcg                                                                                                                    <  1:796084/140‑1 (MQ=255)
 gCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGgcgc                                                                                                                   >  1:1052833/1‑140 (MQ=255)
 gCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGgcgc                                                                                                                   >  1:328093/1‑140 (MQ=255)
  cccTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCa                                                                                                                  >  1:218049/1‑140 (MQ=255)
  cccTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCa                                                                                                                  >  2:689771/1‑140 (MQ=255)
  cccTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCa                                                                                                                  <  1:654697/140‑1 (MQ=255)
  cccTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCa                                                                                                                  >  1:521153/1‑140 (MQ=255)
    cTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGa                                                                                                                >  2:75774/1‑140 (MQ=255)
     tGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGAc                                                                                                               <  1:293660/140‑1 (MQ=255)
       aCCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACaa                                                                                                             >  2:984299/1‑140 (MQ=255)
           aTCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGt                                                                                                         <  1:689771/140‑1 (MQ=255)
                       gACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGa                                                                                             <  1:75774/140‑1 (MQ=255)
                       gACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGa                                                                                             <  2:829196/140‑1 (MQ=255)
                        aCAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTg                                                                                              <  1:603935/138‑1 (MQ=255)
                        aCAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTg                                                                                              >  2:603935/1‑138 (MQ=255)
                           ggCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACggg                                                                                         <  2:218049/140‑1 (MQ=255)
                                  aaCGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAAccc                                                                                  <  1:984299/140‑1 (MQ=255)
                                       tattaGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAGGGTTTTACCAATTGACGGGCTAACCCCGGtc                                                                             <  1:930817/140‑1 (MQ=255)
                                       tattaGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGtc                                                                             <  2:995521/140‑1 (MQ=255)
                                              gCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGtcatcatca                                                                      <  2:1061383/140‑1 (MQ=255)
                                              gCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGtcatcatca                                                                      <  2:525363/140‑1 (MQ=255)
                                                ccAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAac                                                                    >  1:573518/1‑140 (MQ=255)
                                                       cTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGtcatcatca                                                                      >  1:76458/1‑131 (MQ=255)
                                                       cTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGtcatcatca                                                                      <  2:76458/131‑1 (MQ=255)
                                                       cTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGt                                                             >  2:811494/1‑140 (MQ=255)
                                                            gggTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCtgt                                                        <  2:1052833/140‑1 (MQ=255)
                                                                 tAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTg                                                   <  1:287055/140‑1 (MQ=255)
                                                                 tAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTg                                                   >  2:506400/1‑140 (MQ=255)
                                                                   gCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGca                                                 <  1:441287/140‑1 (MQ=255)
                                                                          cgcATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATcgc                                          <  2:573518/140‑1 (MQ=255)
                                                                          cgcATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATcgc                                          <  2:521153/140‑1 (MQ=255)
                                                                             atatCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATcgc                                          >  1:446986/1‑137 (MQ=255)
                                                                             atatCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATcgc                                          <  2:446986/137‑1 (MQ=255)
                                                                                         aTCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATcgcg                                         <  1:595282/126‑1 (MQ=255)
                                                                                         aTCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATcgcg                                         >  2:595282/1‑126 (MQ=255)
                                                                                         aTCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGt                           <  1:506400/140‑1 (MQ=255)
                                                                                         aTCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAA‑CCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTa                          >  2:376153/1‑140 (MQ=255)
                                                                                         aTCAACAACACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATCGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGt                           >  2:308283/1‑140 (MQ=255)
                                                                                           caacaaCACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTAc                         >  2:840180/1‑140 (MQ=255)
                                                                                           caacaaCACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAA‑CCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACg                        <  1:376153/140‑1 (MQ=255)
                                                                                            aacaacACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACg                        <  1:415221/140‑1 (MQ=255)
                                                                                            aacaacACGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACg                        <  1:167809/140‑1 (MQ=255)
                                                                                               aacaCGCGTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAg                     <  2:151891/140‑1 (MQ=255)
                                                                                                    gcgTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCa                                  >  2:1035972/1‑122 (MQ=255)
                                                                                                    gcgTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCa                                  <  1:1035972/122‑1 (MQ=255)
                                                                                                    gcgTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTAc                >  2:413537/1‑140 (MQ=255)
                                                                                                    gcgTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTAc                >  2:923887/1‑140 (MQ=255)
                                                                                                      gTTTATTGGTCTGGCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACgg              >  2:915082/1‑140 (MQ=255)
                                                                                                                  ggCTGATCGCCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATc  >  1:971214/1‑140 (MQ=255)
                                                                                                                          |                                                                                                                                   
CGCCCTGACCAATCAGAATTTCCGACAGGCCATTAACGTTATTAGTGCCCAACAGCTCGTGGGTGTAGCCTTTGCGCATATCGCAGTCGATCAACAACACGCGTTTATTGGTCTGGCTGATCACCGCCGCCAGGTTGGCGCAGACAAAGGTTTTACCAATTGACGGGCTAACCCCGGTCATCATCAACACATTGTTCTGTGCCTGCATCATCGCGAAGTGCAAACTGGTACGCAGACTACGGATGGCTTCAATC  >  NC_000913/2133882‑2134135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑