Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,183,854	T→C	A221A (GCA→GCG)	thiD ←	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,183,854	0	T	C	100.0%	278.7 / NA	78	A221A (GCA→GCG)	thiD	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/45);  total (33/45)

CACCAGGCGTGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGTGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCT  >  NC_000913/2183720‑2183989                                                                                                                                       |                                                                                                                                        caccaGGCGTGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGc                                                                                                                                    <  2:944925/140‑1 (MQ=255)   ccaGGCGTGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCa                                                                                                                                  <  2:677708/140‑1 (MQ=255)    caGGCGTGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCaa                                                                                                                                 >  1:1009770/1‑140 (MQ=255)    caGGCGTGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCaa                                                                                                                                 >  1:553753/1‑140 (MQ=255)     aGGCGTGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAAc                                                                                                                                <  1:3854/140‑1 (MQ=255)      ggCGTGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAAcg                                                                                                                               >  2:901241/1‑140 (MQ=255)      ggCGTGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAAcg                                                                                                                               >  2:630588/1‑140 (MQ=255)      ggCGTGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAAcg                                                                                                                               >  2:334749/1‑140 (MQ=255)        cGTGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAAcgcc                                                                                                                             <  2:553753/140‑1 (MQ=255)          tGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAAcgccgc                                                                                                                           <  1:977315/140‑1 (MQ=255)          tGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATCAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAAcgccgc                                                                                                                           <  2:941463/140‑1 (MQ=255)          gggAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAAcgccgc                                                                                                                           <  2:882221/139‑1 (MQ=255)           ggAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTCCGGTGCCAGCCCAGTTTGTATGGCGC‑GGCGTAGCGCAGCCAACGCCGCag                                                                                                                         <  1:111892/140‑1 (MQ=255)           ggAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCa                                                                                                                          <  2:931459/140‑1 (MQ=255)           ggAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCa                                                                                                                          >  1:684263/1‑140 (MQ=255)             aaGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCaga                                                                                                                        <  1:984142/140‑1 (MQ=255)             aaGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCaga                                                                                                                        <  2:641705/140‑1 (MQ=255)              aGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCGGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGAagag                                                                                                                       <  2:480510/140‑1 (MQ=255)              aGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCagag                                                                                                                       <  2:333452/140‑1 (MQ=255)              aGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCagag                                                                                                                       <  2:684263/140‑1 (MQ=255)              aGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTAGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCagag                                                                                                                       <  2:1009770/140‑1 (MQ=255)                 ggtgAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAAc                                                                                                                                <  1:429362/128‑1 (MQ=255)                 ggtgAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAAc                                                                                                                                >  2:429362/1‑128 (MQ=255)                 ggtgAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAgt                                                                                                                    >  2:365425/1‑140 (MQ=255)                  gtgAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAgtg                                                                                                                   >  2:83487/1‑140 (MQ=255)                  gtgAACCGGAACAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAgtg                                                                                                                   >  1:968946/1‑140 (MQ=255)                   tgAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAgtgt                                                                                                                  <  1:386434/140‑1 (MQ=255)                       ccGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACaa                                                                                                              >  1:531189/1‑140 (MQ=255)                          gACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCa                                                                                                           >  1:315646/1‑140 (MQ=255)                             cAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTg                                                                                                        <  2:52516/140‑1 (MQ=255)                               aTACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGcc                                                                                                      >  1:757710/1‑140 (MQ=255)                                tACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCagag                                                                                                                       <  2:208606/122‑1 (MQ=255)                                tACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCagag                                                                                                                       >  1:208606/1‑122 (MQ=255)                                tACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCg                                                                                                     >  2:231612/1‑140 (MQ=255)                                    gTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTggg                                                                                                 <  2:757710/140‑1 (MQ=255)                                    gTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTggg                                                                                                 >  1:59422/1‑140 (MQ=255)                                     tGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAGCCAGTGCCGTGGgt                                                                                                >  1:80072/1‑140 (MQ=255)                                     tGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGgt                                                                                                >  2:964295/1‑140 (MQ=255)                                     tGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTATACAACCAGTGCCGTGGgt                                                                                                >  2:344302/1‑140 (MQ=255)                                         cAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCC‑CCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGtttt                                                                                           <  1:447039/140‑1 (MQ=255)                                         cAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCC‑CCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGCGCCGTGGGTGtttt                                                                                           <  1:352053/140‑1 (MQ=255)                                          aaCTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGtttt                                                                                           <  1:592168/140‑1 (MQ=255)                                          aaCTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGtttt                                                                                           <  1:83487/140‑1 (MQ=255)                                                 aGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCAt                                                                                    >  1:307346/1‑140 (MQ=255)                                                      gtCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCGGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATgc                                                                               >  2:749619/1‑140 (MQ=255)                                                       tCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATgcg                                                                              <  2:315646/140‑1 (MQ=255)                                                       tCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATgcg                                                                              >  2:917567/1‑140 (MQ=255)                                                       tCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATgcg                                                                              <  2:307346/140‑1 (MQ=255)                                                       gcGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATgcg                                                                              <  2:160270/139‑1 (MQ=255)                                                         ggCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGcgcg                                                                            <  2:428250/140‑1 (MQ=255)                                                         ggCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGcgcg                                                                            <  1:749619/140‑1 (MQ=255)                                                          gCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCgcgg                                                                           <  1:704572/140‑1 (MQ=255)                                                                 cTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTa                                                                    <  2:1044394/140‑1 (MQ=255)                                                                    aCGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAAc                                                                 >  2:313077/1‑140 (MQ=255)                                                                     cGCCGATGAAAGCCGGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAAcc                                                                >  1:307821/1‑140 (MQ=255)                                                                          aTGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCgttgt                                                           >  2:432365/1‑140 (MQ=255)                                                                           tGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCgttgtt                                                          <  1:231612/140‑1 (MQ=255)                                                                               aGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCAcc                                                      <  2:881621/140‑1 (MQ=255)                                                                                 ccAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCt                                                    <  2:59422/140‑1 (MQ=255)                                                                                          tGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTaa                                           <  1:313077/140‑1 (MQ=255)                                                                                               cctGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGc                                      >  1:646648/1‑140 (MQ=255)                                                                                                   tACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGt                                  <  1:38664/140‑1 (MQ=255)                                                                                                        tgtCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCggg                             <  1:432365/140‑1 (MQ=255)                                                                                                        tgtCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCggg                             >  1:598965/1‑140 (MQ=255)                                                                                                        tgtCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCggg                             >  1:244352/1‑140 (MQ=255)                                                                                                          tCAGCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCt                           >  1:18061/1‑140 (MQ=255)                                                                                                             gCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTg                        >  1:751697/1‑140 (MQ=255)                                                                                                             gCCCAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTg                        >  2:908066/1‑140 (MQ=255)                                                                                                               ccAGTTTGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCt                      <  2:751697/140‑1 (MQ=255)                                                                                                                    ttGTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCtcatca                 <  1:365425/140‑1 (MQ=255)                                                                                                                      gTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCatcatc               <  1:908066/140‑1 (MQ=255)                                                                                                                      gTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCatcatc               <  1:917567/140‑1 (MQ=255)                                                                                                                      gTATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGCACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCatcatc               <  2:541841/140‑1 (MQ=255)                                                                                                                       tATGGCGCGGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATcc              >  1:661324/1‑140 (MQ=255)                                                                                                                            cgcgGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATg         >  1:100763/1‑140 (MQ=255)                                                                                                                              cgGGCGTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGac       <  2:838886/140‑1 (MQ=255)                                                                                                                                  cGTAGCGCAGCC‑ACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCt  <  2:909402/140‑1 (MQ=255)                                                                                                                                   gTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCt  <  2:244352/140‑1 (MQ=255)                                                                                                                                   gTAGCGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCt  <  1:964295/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CACCAGGCGTGGAAGTGGTGAACCGGACCAATACCGTGACCAACTTCCAGCGTGTCGGCCTGGGCTAACGCCGATGAAAGCCAGCTTTTTGCCTCCTGTACGGTGTCAGCCCAGTTTGTATGGCGCGGGCGTAGTGCAGCCAACGCCGCAGAGAGTGTACAACCAGTGCCGTGGGTGTTTTTGGTCATAATGCGCGGTGCGGTAAACCGTTGTTCACCCTCGCGGGTAAACAGCCAGTCCGGGCTTTGCTCATCATCCAGATGACCACCT  >  NC_000913/2183720‑2183989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑