Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,357,116	C→T	S71S (AGC→AGT)	rpnE →	inactive recombination‑promoting nuclease‑like protein RpnE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,357,116	0	C	T	100.0%	141.5 / NA	41	S71S (AGC→AGT)	rpnE	inactive recombination‑promoting nuclease‑like protein RpnE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/25);  total (16/25)

GATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAG  >  NC_000913/2356979‑2357243                                                                                                                                          |                                                                                                                                gATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGa                                                                                                                               <  2:822961/140‑1 (MQ=255)       ctcGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGa                                                                                                                         <  2:308407/140‑1 (MQ=255)       ctcGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGa                                                                                                                         <  1:1004769/140‑1 (MQ=255)       ctcGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGa                                                                                                                         <  2:819086/140‑1 (MQ=255)         cGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAgtgacggtgacg                                                                                                                       <  2:409108/140‑1 (MQ=255)         cGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAgtgacggtgacg                                                                                                                       <  1:1021616/140‑1 (MQ=255)         cGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAgtgacggtgacg                                                                                                                       <  1:916314/140‑1 (MQ=255)         cGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAgtgacggtgacg                                                                                                                       <  2:917840/140‑1 (MQ=255)         cGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAgtgacggtgacg                                                                                                                       <  1:173199/140‑1 (MQ=255)         cGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAgtgacggtgacg                                                                                                                       <  2:922322/140‑1 (MQ=255)           aaaTACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGc                                                                                                                     >  1:287026/1‑140 (MQ=255)           aaaTACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGc                                                                                                                     >  1:591614/1‑140 (MQ=255)           aaaTACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGc                                                                                                                     <  1:67254/140‑1 (MQ=255)                 aTTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATc                                                                                                               <  2:791118/140‑1 (MQ=255)                      ccAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTg                                                                                                          >  1:547951/1‑140 (MQ=255)                        aGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCg                                                                                                        <  2:547951/140‑1 (MQ=255)                        aGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCg                                                                                                        <  2:236180/140‑1 (MQ=255)                          aaCCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGc                                                                                                                     <  2:424907/125‑1 (MQ=255)                          aaCCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGc                                                                                                                     >  1:424907/1‑125 (MQ=255)                          aaCCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTg                                                                                                      >  2:874053/1‑140 (MQ=255)                           aCCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGa                                                                                                     >  2:455933/1‑140 (MQ=255)                              aCTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTg                                                                                                  <  2:711559/140‑1 (MQ=255)                              aCTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTg                                                                                                  <  1:455933/140‑1 (MQ=255)                               cTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGa                                                                                                 >  1:747972/1‑140 (MQ=255)                               cTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGa                                                                                                 >  1:939118/1‑140 (MQ=255)                                     aaGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCa                                                                                           <  2:754040/140‑1 (MQ=255)                                     aaGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCa                                                                                           <  2:10696/140‑1 (MQ=255)                                          ttGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGct                                                                                      <  2:984704/140‑1 (MQ=255)                                            gCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGctct                                                                                    >  1:898159/1‑140 (MQ=255)                                             cAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTg                                                                                   <  1:874053/140‑1 (MQ=255)                                             cAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTg                                                                                   <  2:591614/140‑1 (MQ=255)                                             cAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTg                                                                                   <  2:517872/140‑1 (MQ=255)                                             cAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTg                                                                                   <  2:287026/140‑1 (MQ=255)                                                            aCGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATgg                                                                    >  2:852713/1‑140 (MQ=255)                                                                        cACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTaa                                                        >  2:458982/1‑140 (MQ=255)                                                                        cACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTaa                                                        <  1:889316/140‑1 (MQ=255)                                                                        cACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTaa                                                        >  1:383075/1‑140 (MQ=255)                                                                         aCTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAAt                                                       >  1:823474/1‑140 (MQ=255)                                                                          cTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATg                                                      <  2:823474/140‑1 (MQ=255)                                                                                       aaaaaGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTg                                         >  1:883480/1‑140 (MQ=255)                                                                                          aaGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTgccg                                      <  2:883480/140‑1 (MQ=255)                                                                                               tACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATg                                 <  1:852713/140‑1 (MQ=255)                                                                                                     cTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGt                           <  2:383075/140‑1 (MQ=255)                                                                                                              cGGATGTTTTGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGAc                  <  1:204298/140‑1 (MQ=255)                                                                                                                      ttGTGGTCCGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTa          >  1:94612/1‑140 (MQ=255)                                                                                                                             ccGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACaga   <  1:285684/140‑1 (MQ=255)                                                                                                                              cGTGGAAACCAGTGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACagag  >  2:1052446/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                GATTTTCTCGAAATACATTTACCAGAACCACTGCGCAAGCTTTGCAACCTGCAAACCTTACGCCTGGAACCCACTAGTTTTATTGAAAAAAGTTTACGCGCTTACTACTCGGATGTTTTGTGGTCCGTGGAAACCAGCGACGGTGACGGCTATATCTACTGCGTGATTGAACATCAAAGCTCTGCAGAAAAGAATATGGCTTTTCGGCTAATGCGCTATGCCACTGCCGCCATGCAGCGTCACCAGGACAAAGGCTATGACAGAG  >  NC_000913/2356979‑2357243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑