Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,376,205	T→C	V205V (GTA→GTG)	menB ←	1,4‑dihydroxy‑2‑naphthoyl‑CoA synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,376,205	0	T	C	100.0%	209.2 / NA	58	V205V (GTA→GTG)	menB	1,4‑dihydroxy‑2‑naphthoyl‑CoA synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/32);  total (26/32)

CCCGCCAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGTACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAG  >  NC_000913/2376068‑2376337                                                                                                                                          |                                                                                                                                     cccGCCAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCAc                                                                                                                                    >  2:313232/1‑140 (MQ=255)  ccGCCAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCAcc                                                                                                                                   <  2:163548/140‑1 (MQ=255)  ccGCCAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCAcc                                                                                                                                   <  1:313232/140‑1 (MQ=255)   cGCCAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCa                                                                                                                                  >  2:278862/1‑140 (MQ=255)   cGCCAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCa                                                                                                                                  <  2:410656/140‑1 (MQ=255)   cGCCAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCa                                                                                                                                  <  1:344945/140‑1 (MQ=255)   cGCCAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCa                                                                                                                                  <  1:431282/140‑1 (MQ=255)   cGCCAGCTCCTGCAGCCCCGCCTGCCCATCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCa                                                                                                                                  <  1:376218/140‑1 (MQ=255)     ccAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCa                                                                                                                                  >  2:906739/1‑138 (MQ=255)     ccAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCa                                                                                                                                  <  1:906739/138‑1 (MQ=255)             tGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTcac                                                                                                                        <  2:521255/140‑1 (MQ=255)              gCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTcaca                                                                                                                       <  1:609749/140‑1 (MQ=255)               cAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACaa                                                                                                                      >  1:530892/1‑140 (MQ=255)               cAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACaa                                                                                                                      <  1:557555/140‑1 (MQ=255)               cAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACaa                                                                                                                      <  2:332487/140‑1 (MQ=255)               cAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACaa                                                                                                                      <  1:138189/140‑1 (MQ=255)                  ccccGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGc                                                                                                                   <  1:409615/140‑1 (MQ=255)                  ccccGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGc                                                                                                                   >  2:288307/1‑140 (MQ=255)                  ccccGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGc                                                                                                                   >  1:971007/1‑140 (MQ=255)                  ccccGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGc                                                                                                                   >  2:52865/1‑140 (MQ=255)                   cccGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGcc                                                                                                                  >  2:1004592/1‑140 (MQ=255)                      gCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCat                                                                                                               >  1:637236/1‑140 (MQ=255)                       ccTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCata                                                                                                              >  2:877910/1‑140 (MQ=255)                       ccTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCata                                                                                                              >  1:319848/1‑140 (MQ=255)                                 acaGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCt                                                                                                    <  1:52865/140‑1 (MQ=255)                                   aGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGt                                                                                                  >  2:660335/1‑140 (MQ=255)                                    gTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGtt                                                                                                 <  1:660335/140‑1 (MQ=255)                                     tCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGttt                                                                                                >  2:48075/1‑140 (MQ=255)                                      cGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGtttt                                                                                               <  1:278862/140‑1 (MQ=255)                                        gCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGtt                                                                                                 <  1:756414/136‑1 (MQ=255)                                        gCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGtt                                                                                                 >  2:756414/1‑136 (MQ=255)                                        gCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGc                                                                                             >  2:82690/1‑140 (MQ=255)                                         cGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGcg                                                                                            <  1:82690/140‑1 (MQ=255)                                           ttCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGcgtc                                                                                          <  1:309233/140‑1 (MQ=255)                                                   aGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCTCCAACGGACCGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGAc                                                                                  <  2:971007/140‑1 (MQ=255)                                                         cAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGcaca                                                                            <  1:877910/140‑1 (MQ=255)                                                          aGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAg                                                                           >  1:364153/1‑140 (MQ=255)                                                               gcgcagcgcCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAAcc                                                                      >  1:933225/1‑140 (MQ=255)                                                                cgcagcgcCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAAc                                                                       <  2:696411/138‑1 (MQ=255)                                                                cgcagcgcCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAAc                                                                       >  1:696411/1‑138 (MQ=255)                                                                     cgcCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGAttt                                                                >  1:290291/1‑140 (MQ=255)                                                                        cATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCAc                                                             <  1:1004592/140‑1 (MQ=255)                                                                          tCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCAcgc                                                           >  1:1008683/1‑140 (MQ=255)                                                                          tCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCAcgc                                                           >  1:994177/1‑140 (MQ=255)                                                                               cTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCttt                                                      >  1:819240/1‑140 (MQ=255)                                                                                          caTTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCcga                                           <  2:530892/140‑1 (MQ=255)                                                                                            tttCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCcgacg                                         >  1:801781/1‑140 (MQ=255)                                                                                               cgcgGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATg                                      >  2:1020071/1‑140 (MQ=255)                                                                                                gcgGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGc                                     <  1:48075/140‑1 (MQ=255)                                                                                                  ggCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGa                                   <  2:921535/140‑1 (MQ=255)                                                                                                  gccACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCAACACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGa                                   <  2:1004392/138‑1 (MQ=255)                                                                                                   gCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAg                                  <  2:819240/140‑1 (MQ=255)                                                                                                    cACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGc                                 <  2:1008683/140‑1 (MQ=255)                                                                                                    cACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGc                                 <  1:127800/140‑1 (MQ=255)                                                                                                    cACCAACGGACGGTTTCTTTTTCCAGATCCACCAGTGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGc                                 <  1:563943/140‑1 (MQ=255)                                                                                                             acggTTTCTTTTTCCAGATCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGa                        <  2:290291/140‑1 (MQ=255)                                                                                                                              aTCCGCCAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGt       >  2:112879/1‑140 (MQ=255)                                                                                                                                   ccAGCGGCACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAg  >  2:113696/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     CCCGCCAGCTCCTGCAGCCCCGCCTGCCCGTCACAGTCGGCGTTCAGTGCAGCTTTCAGGCAGCGCAGCGCCATCGGGCTGTTTTGCAGCATTTCGCGGCACCAACGGACGGTTTCTTTTTCCAGATCCGCCAGCGGTACCACGGTGTTCACAAGGCCCATATCCAGCGCCTGTTTTGCGTCGTACTGACGGCACAGGAACCAGATTTCACGCGCTTTTTTCTGCCCGACGATGCGAGCCATGTAGGAAGCGCCCCAGCCGCCGTCGAAG  >  NC_000913/2376068‑2376337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑