Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,430,853	G→A	intergenic (‑90/+169)	cvpA ← / ← dedD	colicin V production protein/cell division protein DedD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,430,853	0	G	A	100.0%	149.3 / NA	43	intergenic (‑90/+169)	cvpA/dedD	colicin V production protein/cell division protein DedD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/22);  total (21/22)

GAGGAAAAAGCAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATA  >  NC_000913/2430717‑2430988                                                                                                                                         |                                                                                                                                        gagGAAAAAGCAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACAt                                                                                                                                      >  2:895381/1‑140 (MQ=255)     aaaaaGCAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAAt                                                                                                                                  >  1:880572/1‑140 (MQ=255)     aaaaaGCAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAAt                                                                                                                                  >  1:782477/1‑140 (MQ=255)              tCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTACCTTCCCGCACATCAATAAAAATGgc                                                                                                                         >  1:758244/1‑140 (MQ=255)                  cgcgATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTg                                                                                                                     >  2:664937/1‑140 (MQ=255)                                        cAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGa                                                                                               <  2:658029/140‑1 (MQ=255)                                        cAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGa                                                                                               <  2:679655/140‑1 (MQ=255)                                               tatGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTa                                                                                        <  2:393375/140‑1 (MQ=255)                                                atGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTAt                                                                                       >  1:312458/1‑140 (MQ=255)                                                    gtCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCt                                                                                   >  2:800532/1‑140 (MQ=255)                                                     tCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCtt                                                                                  >  1:378316/1‑140 (MQ=255)                                                       ccACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTg                                                                                <  1:689977/140‑1 (MQ=255)                                                         aCGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCg                                                                              <  2:1005870/140‑1 (MQ=255)                                                          cGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGc                                                                                     >  1:174746/1‑132 (MQ=255)                                                          cGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGc                                                                                     <  2:174746/132‑1 (MQ=255)                                                          cGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCgg                                                                             >  1:945436/1‑140 (MQ=255)                                                           gATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGc                                                                            <  1:664937/140‑1 (MQ=255)                                                           gATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGc                                                                            <  1:688738/140‑1 (MQ=255)                                                            aTTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCa                                                                           <  2:334164/140‑1 (MQ=255)                                                            aTTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCa                                                                           <  2:945436/140‑1 (MQ=255)                                                             ttttACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCAt                                                                          <  2:758244/140‑1 (MQ=255)                                                                         tCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACACCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCgt                                                              >  2:364450/1‑140 (MQ=255)                                                                               tGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTACGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAAGATTCAACGCCATCAACTTTTTATGCCTGTGCGGCATCGGGCAATGCGTGTCgaa                                                        >  2:959447/1‑138 (MQ=255)                                                                                 ttCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATg                                                      >  1:841140/1‑140 (MQ=255)                                                                                   cGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATgcg                                                    >  1:637136/1‑140 (MQ=255)                                                                                   cGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATgcg                                                    <  2:782477/140‑1 (MQ=255)                                                                                   cGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATg                                                      <  2:296708/138‑1 (MQ=255)                                                                                   cGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATg                                                      >  1:296708/1‑138 (MQ=255)                                                                                          cATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTaaa                                             >  1:1075918/1‑140 (MQ=255)                                                                                           aTTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAAc                                            >  2:313971/1‑140 (MQ=255)                                                                                                  cAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTAt                                     <  1:915358/140‑1 (MQ=255)                                                                                                   agaaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATc                                    <  2:378316/140‑1 (MQ=255)                                                                                                     aaaaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCg                                  <  1:596743/140‑1 (MQ=255)                                                                                                       aaagaaaaCGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGAc                                >  2:1060638/1‑140 (MQ=255)                                                                                                          gaaaaCGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTa                             <  2:818991/140‑1 (MQ=255)                                                                                                          gaaaaCGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTa                             <  1:197325/140‑1 (MQ=255)                                                                                                          gaaaaCGTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTa                             <  1:1060638/140‑1 (MQ=255)                                                                                                                gTTTGCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTc                       <  1:384550/140‑1 (MQ=255)                                                                                                                    gCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTa                             <  1:927722/130‑1 (MQ=255)                                                                                                                    gCGTAGGGATTTCCTTCCCGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTa                             >  2:927722/1‑130 (MQ=255)                                                                                                                                     ccGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATa  <  2:177759/140‑1 (MQ=255)                                                                                                                                     ccGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATa  >  2:69019/1‑140 (MQ=255)                                                                                                                                     ccGCACATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATa  >  2:924419/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GAGGAAAAAGCAATCACCGCGATTATGGCGTAATCAATCCAGACCATATGTGTCCCACGATTTTACGCCCTGTCATCCTGTTCGGGGCGCATTCTAACAGAAAAAGAAAACGTTTGCGTAGGGATTTCCTTCCCGCGCATCAATAAAAATGGCGCTGAAAAAATATTCAACGCCATCGACTTTTTATGCCTTTGCGGCATCGGGCAATGCGTGTCGGATGCGGCGTAAACGCCTTATCCGACCTACGGTTCTACCCCTGCGTAGGCCTGATA  >  NC_000913/2430717‑2430988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑