Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,438,498	G→A	T183T (ACG→ACA)	flk →	putative flagella assembly protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,438,498	0	G	A	100.0%	254.1 / NA	72	T183T (ACG→ACA)	flk	putative flagella assembly protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/31);  total (41/31)

TTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAA  >  NC_000913/2438368‑2438632                                                                                                                                   |                                                                                                                                       ttCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAAccc                                                                                                                               >  1:228120/1‑140 (MQ=255)  tctcACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCa                                                                                                                              <  2:475226/140‑1 (MQ=255)         cTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGa                                                                                                                                   >  2:819014/1‑128 (MQ=255)         cTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGa                                                                                                                                   <  1:819014/128‑1 (MQ=255)           gTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACaggg                                                                                                                                     >  2:278114/1‑124 (MQ=255)           gTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACaggg                                                                                                                                     <  1:278114/124‑1 (MQ=255)            ttGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGa                                                                                                                                   >  1:549344/1‑125 (MQ=255)            ttGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGa                                                                                                                                   <  2:549344/125‑1 (MQ=255)            ttGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGAt                                                                                                                    >  1:339096/1‑140 (MQ=255)            ttGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGAt                                                                                                                    >  1:957308/1‑140 (MQ=255)                    agggaagATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAAt                                                                                                            <  2:339096/140‑1 (MQ=255)                    agggaagATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAAt                                                                                                            <  1:499744/140‑1 (MQ=255)                      ggaagATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCg                                                                                                          >  2:483453/1‑140 (MQ=255)                      ggaagATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCg                                                                                                          >  2:718928/1‑140 (MQ=255)                           aTGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCt                                                                                                     <  1:790408/140‑1 (MQ=255)                             ggTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTA‑CGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGa                                                                                                  >  1:332224/1‑140 (MQ=255)                                tATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAAc                                                                                                >  2:679384/1‑140 (MQ=255)                                 aTTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACt                                                                                               >  1:170959/1‑140 (MQ=255)                                 aTTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACt                                                                                               >  1:467211/1‑140 (MQ=255)                                  ttCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCCAACAGATCTGGCAATCGATGCTGGAACtt                                                                                              >  1:517093/1‑140 (MQ=255)                                   tCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTA‑CGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTc                                                                                            <  2:332224/140‑1 (MQ=255)                                     cGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTcc                                                                                           >  1:254242/1‑140 (MQ=255)                                      gCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCg                                                                                          <  1:369237/140‑1 (MQ=255)                                       cAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTcc                                                                                           >  2:1056867/1‑138 (MQ=255)                                       cAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTcc                                                                                           <  1:1056867/138‑1 (MQ=255)                                       cAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCgg                                                                                         >  1:200263/1‑140 (MQ=255)                                       cAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCGGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCgg                                                                                         >  2:985841/1‑140 (MQ=255)                                             cagcagCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGa                                                                                    >  2:363824/1‑139 (MQ=255)                                             cagcagCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGa                                                                                    <  1:363824/139‑1 (MQ=255)                                                    gcgAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGc                                                                            <  2:254242/140‑1 (MQ=255)                                                    gcgAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGc                                                                            >  2:812973/1‑140 (MQ=255)                                                    gcgAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGc                                                                            >  1:356895/1‑140 (MQ=255)                                                    gcgAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGc                                                                            >  2:1057035/1‑140 (MQ=255)                                                    gcgAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGc                                                                            >  1:808849/1‑140 (MQ=255)                                                        ggCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATgg                                                                             <  1:255290/135‑1 (MQ=255)                                                        ggCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATgg                                                                             >  2:255290/1‑135 (MQ=255)                                                        ggCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGc                                                                            <  2:719862/136‑1 (MQ=255)                                                        ggCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGc                                                                            >  1:719862/1‑136 (MQ=255)                                                               gaccgTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTcc                                                                 <  2:170959/140‑1 (MQ=255)                                                                    tCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGa                                                            >  1:437395/1‑140 (MQ=255)                                                                    tCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGa                                                            <  2:517093/140‑1 (MQ=255)                                                                    tCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGa                                                            <  1:718928/140‑1 (MQ=255)                                                                      cTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGaaa                                                          <  1:985841/140‑1 (MQ=255)                                                                      cTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGaaa                                                          <  2:228120/140‑1 (MQ=255)                                                                           ttaCCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAGGATGGCGAGTTAATTCCAGCGAAACTGtt                                                     <  1:1076344/140‑1 (MQ=255)                                                                           ttaCCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGaaa                                                          <  2:871240/135‑1 (MQ=255)                                                                           ttaCCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGaaa                                                          >  1:871240/1‑135 (MQ=255)                                                                           ttaCCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGtt                                                     <  1:83650/140‑1 (MQ=255)                                                                               cggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAAc                                                 >  1:784265/1‑140 (MQ=255)                                                                               cggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAAc                                                 >  2:951395/1‑140 (MQ=255)                                                                                ggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAAcc                                                >  2:299495/1‑140 (MQ=255)                                                                                ggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAAcc                                                <  1:147687/140‑1 (MQ=255)                                                                                ggcggAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAAcc                                                <  1:21674/140‑1 (MQ=255)                                                                                     aGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTg                                           >  2:661749/1‑140 (MQ=255)                                                                                       cacaATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGt                                         <  1:135122/140‑1 (MQ=255)                                                                                          aaTGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                      <  1:590837/140‑1 (MQ=255)                                                                                          aaTGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                      <  1:812973/140‑1 (MQ=255)                                                                                            tGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCt                                    >  1:576915/1‑140 (MQ=255)                                                                                               gcTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTg                                           >  1:733397/1‑130 (MQ=255)                                                                                               gcTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTg                                           <  2:733397/130‑1 (MQ=255)                                                                                               gcTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGc                                 >  1:442929/1‑140 (MQ=255)                                                                                                 tAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                      <  1:734623/133‑1 (MQ=255)                                                                                                 tAAAACAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGAc                                      >  2:734623/1‑133 (MQ=255)                                                                                                    aaCAGCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAg                            >  1:974208/1‑140 (MQ=255)                                                                                                        gCTGGTGACCAAACTTGCGGCGGCAACAGGTGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGgcgc                        >  1:671367/1‑140 (MQ=255)                                                                                                        gCTGGTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGgcgc                        >  1:525143/1‑140 (MQ=255)                                                                                                           ggTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTc                     >  2:817950/1‑140 (MQ=255)                                                                                                            gTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCa                    <  1:9674/140‑1 (MQ=255)                                                                                                            gTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCa                    <  2:442929/140‑1 (MQ=255)                                                                                                            gTGACCAAACTTGCGGCGGCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCACGTCa                    <  1:661749/140‑1 (MQ=255)                                                                                                                         cggcggCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAg       >  2:518141/1‑140 (MQ=255)                                                                                                                         cggcggCAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAg       >  1:1015757/1‑140 (MQ=255)                                                                                                                              gcAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCaaaa  >  2:1013498/1‑140 (MQ=255)                                                                                                                              gcAACAGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCaaaa  >  1:422785/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                       TTCTCACCCTGTTGCAGGAAGGGAAGATGGTTATTCCGCAACCACAGCAGCGCGAGGCGACCGACCGTCCTTTATTACCGGCGGAGCACAATGCGCTAAAACAGCTGGTGACCAAACTTGCGGCGGCAACGGGGGAACCCAGCAAACAGATCTGGCAATCGATGCTGGAACTTTCCGGGGTGAAAGATGGCGAGTTAATTCCAGCGAAACTGTTTAACCATCTGGTGACCTGGCTACAGGCGCGTCAGACGCTAAGCCAGCAAAA  >  NC_000913/2438368‑2438632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑