Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,464,616	T→C	H131R (CAC→CGC)	mlaA ←	intermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,464,616	0	T	C	100.0%	226.1 / NA	63	H131R (CAC→CGC)	mlaA	intermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/31);  total (32/31)

CCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCAAGAAGTAGT  >  NC_000913/2464483‑2464751                                                                                                                                      |                                                                                                                                        ccAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGtt                                                                                                                                   >  1:1062592/1‑140 (MQ=255) ccAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGtt                                                                                                                                   >  1:894125/1‑140 (MQ=255)  cAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTc                                                                                                                                  >  2:550628/1‑140 (MQ=255)     gAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGt                                                                                                                               <  1:134420/140‑1 (MQ=255)          aaCCGGGTAAAACCCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGtt                                                                                                                          <  1:896714/140‑1 (MQ=255)          aaCCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGtt                                                                                                                          >  2:647648/1‑140 (MQ=255)           aCCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTg                                                                                                                         >  2:282662/1‑140 (MQ=255)           aCCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTg                                                                                                                         >  1:218727/1‑140 (MQ=255)                   aaacCCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCg                                                                                                                 <  2:1009579/140‑1 (MQ=255)                     aaCCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCggg                                                                                                               <  1:57942/140‑1 (MQ=255)                          tCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGc                                                                                                          <  2:863258/140‑1 (MQ=255)                          tCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGc                                                                                                          >  2:5699/1‑140 (MQ=255)                          tCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGc                                                                                                          >  1:642359/1‑140 (MQ=255)                           ccGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGcc                                                                                                         <  1:647648/140‑1 (MQ=255)                            cGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCa                                                                                                        <  2:116694/140‑1 (MQ=255)                             gCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCAt                                                                                                       <  1:550628/140‑1 (MQ=255)                                   tCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGc                                                                                                 <  1:5699/140‑1 (MQ=255)                                      cccccGTCATCCGGTATCCTGAGGCTACCGTAGAACGGTATCTGCACCTAGGGCCCATAGCCCACCTCATAATGGCCACGCGTACTACCGAAACGGCGAGGTTCAGTCGGTTGCAGTTTCGGGTTCGCCATCCCGGCAAc                                                                                              <  2:703834/137‑1 (MQ=255)                                      ccaccGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAAc                                                                                              <  2:218727/140‑1 (MQ=255)                                      ccaccGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAAc                                                                                              <  2:775832/140‑1 (MQ=255)                                       caccGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACa                                                                                             >  2:334397/1‑140 (MQ=255)                                         ccGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATc                                                                                           >  2:763619/1‑140 (MQ=255)                                            tcatcaCGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGcc                                                                                                         <  2:724536/123‑1 (MQ=255)                                            tcatcaCGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGcc                                                                                                         >  1:724536/1‑123 (MQ=255)                                            tcatcaCGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTg                                                                                                  >  2:917211/1‑130 (MQ=255)                                            tcatcaCGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTg                                                                                                  <  1:917211/130‑1 (MQ=255)                                            tcatcaCGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAAt                                                                                        >  2:316692/1‑140 (MQ=255)                                            tcatcaCGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAAt                                                                                        >  1:625376/1‑140 (MQ=255)                                              atcaCGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATaa                                                                                      >  1:290240/1‑140 (MQ=255)                                                     aGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGcc                                                                               <  1:142892/140‑1 (MQ=255)                                                     aGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGcc                                                                               <  1:549870/140‑1 (MQ=255)                                                     aGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGcc                                                                               <  1:908400/140‑1 (MQ=255)                                                       cGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAAc                                                                                              <  1:587446/123‑1 (MQ=255)                                                       cGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAAc                                                                                              >  2:587446/1‑123 (MQ=255)                                                       cGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGccc                                                                              >  1:545912/1‑139 (MQ=255)                                                       cGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGccc                                                                              <  2:545912/139‑1 (MQ=255)                                                       cGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCa                                                                             >  1:878748/1‑140 (MQ=255)                                                        gTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCAt                                                                            >  1:261937/1‑140 (MQ=255)                                                                  cGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATg                                                                  <  1:458781/140‑1 (MQ=255)                                                                  cGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATg                                                                  <  2:878748/140‑1 (MQ=255)                                                                                 gAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAg                                                   >  1:187832/1‑140 (MQ=255)                                                                                    cGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCgg                                                >  1:659242/1‑140 (MQ=255)                                                                                    cGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCgg                                                >  2:860835/1‑140 (MQ=255)                                                                                                 aCCCACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCa                                   <  2:625376/140‑1 (MQ=255)                                                                                                    cACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGAcc                                    >  1:434874/1‑136 (MQ=255)                                                                                                    cACGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGAcc                                    <  2:434874/136‑1 (MQ=255)                                                                                                     aCGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATccc                               <  1:763619/140‑1 (MQ=255)                                                                                                     aCGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATccc                               >  1:842678/1‑140 (MQ=255)                                                                                                      cGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGAcc                                    >  2:423395/1‑134 (MQ=255)                                                                                                      cGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGAcc                                    <  1:423395/134‑1 (MQ=255)                                                                                                      cGCCATAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATcccc                              >  1:753345/1‑140 (MQ=255)                                                                                                          aTAATGACCAAGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGAt                          <  1:845564/140‑1 (MQ=255)                                                                                                                   aaGCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGc                 >  1:501058/1‑140 (MQ=255)                                                                                                                     gCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGccc               >  1:416671/1‑140 (MQ=255)                                                                                                                     gCGTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGccc               <  2:290240/140‑1 (MQ=255)                                                                                                                       gTACTACCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTg             >  2:369086/1‑140 (MQ=255)                                                                                                                            acCGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCaaga        <  1:334397/140‑1 (MQ=255)                                                                                                                             ccGAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCaagaa       <  1:756958/140‑1 (MQ=255)                                                                                                                               gAAGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCAAGAagt     <  1:316692/140‑1 (MQ=255)                                                                                                                                aaGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGc                 <  1:293240/127‑1 (MQ=255)                                                                                                                                aaGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGc                 >  2:293240/1‑127 (MQ=255)                                                                                                                                aaGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCAAGAagta    >  2:1060374/1‑140 (MQ=255)                                                                                                                                aaGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCAAGAagta    >  1:870689/1‑140 (MQ=255)                                                                                                                                 aGCGGCGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCAAGAagtag   <  2:659242/140‑1 (MQ=255)                                                                                                                                  gcggcgAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCAAGAagtagt  >  2:807467/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        CCAGGAAAGAACCGGGTAAAAACCATCCGCCATATCACCACCGTCATCACGCAGCGTGAAGCTACCGTAGAACGGTAACTGAACGTAAGGCCCATAACCCACGCCATAATGACCAAGCGTACTACCGAAGCGGTGAGGTTCAGTCCGTTGCAGTTTCGGGTTCGCCATCCCTGCAACATCAATAAAACCGCCCATCCCCAAAATGGTGTTCAGGAAAAAGCGGGTAAAGTGGACCATCCCCTGATAAGGGTCGCCCTGCAAGAAGTAGT  >  NC_000913/2464483‑2464751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑