Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,505,468	A→G	L27P (CTG→CCG)	ypdE ←	broad‑specificity exoaminopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,505,468	0	A	G	100.0%	217.0 / NA	61	L27P (CTG→CCG)	ypdE	broad‑specificity exoaminopeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/30);  total (31/30)

GCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTGCG  >  NC_000913/2505337‑2505601                                                                                                                                    |                                                                                                                                      gcACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATc                                                                                                                               <  2:821147/140‑1 (MQ=255)     cATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGcc                                                                                                                           >  2:369392/1‑140 (MQ=255)            cccACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTc                                                                                                                    <  1:369392/140‑1 (MQ=255)            cccACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTc                                                                                                                    <  1:292499/140‑1 (MQ=255)             ccACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTcc                                                                                                                   <  2:578327/140‑1 (MQ=255)               aCTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTg                                                                                                                 >  1:699259/1‑140 (MQ=255)                              gcACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCTCTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGAt                                                                                                  <  1:425590/140‑1 (MQ=255)                               cacaGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGAt                                                                                                  <  1:43582/139‑1 (MQ=255)                               cacaGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGAt                                                                                                  >  2:43582/1‑139 (MQ=255)                               cacaGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATc                                                                                                 >  1:1032151/1‑140 (MQ=255)                                   gATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCAt                                                                                             >  1:37160/1‑140 (MQ=255)                                    atcatcACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATc                                                                                            >  2:564677/1‑140 (MQ=255)                                      catcaCCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTg                                                                                          <  1:740303/140‑1 (MQ=255)                                        tcaCCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGcc                                                                                        >  1:707436/1‑140 (MQ=255)                                         caCCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCt                                                                                       <  2:1032151/140‑1 (MQ=255)                                          aCCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTc                                                                                      >  1:195174/1‑140 (MQ=255)                                           ccTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCg                                                                                     <  2:715526/140‑1 (MQ=255)                                              tCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGAt                                                                                                  <  2:482247/124‑1 (MQ=255)                                              tCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGAt                                                                                                  >  1:482247/1‑124 (MQ=255)                                              tCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCTTCTGCCTCGCTc                                                                                  >  2:867325/1‑140 (MQ=255)                                              tCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTc                                                                                  >  2:148693/1‑140 (MQ=255)                                              tCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTc                                                                                  >  1:239652/1‑140 (MQ=255)                                               cGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCa                                                                                 <  2:942715/140‑1 (MQ=255)                                               cGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCa                                                                                 <  2:239652/140‑1 (MQ=255)                                               cGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCa                                                                                 <  1:1079987/140‑1 (MQ=255)                                                 gACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAAc                                                                               <  2:50691/140‑1 (MQ=255)                                                  aCCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACg                                                                              >  1:431296/1‑140 (MQ=255)                                                      gTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCttt                                                                          >  1:691764/1‑140 (MQ=255)                                                           ttcattcaGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATa                                                                     <  2:1071223/140‑1 (MQ=255)                                                              attcaGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCg                                                                  <  2:691764/140‑1 (MQ=255)                                                                  aGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATaa                                                              <  2:707436/140‑1 (MQ=255)                                                                        aTCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCAt                                                        <  1:477755/140‑1 (MQ=255)                                                                             cACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGc                                                   >  1:380462/1‑140 (MQ=255)                                                                                  aTCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCtctc                                              >  2:13010/1‑140 (MQ=255)                                                                                  aTCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCtctc                                              >  2:465305/1‑140 (MQ=255)                                                                                  aTCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCtctc                                              >  1:1079500/1‑140 (MQ=255)                                                                                  aTCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCtctc                                              >  2:274946/1‑140 (MQ=255)                                                                                  aTCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCtctc                                              >  2:1045588/1‑140 (MQ=255)                                                                                  aTCCCAGACCATCAAATCACACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATACATTATGCCtctc                                              >  2:787862/1‑140 (MQ=255)                                                                                         aCCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAg                                       <  2:380462/140‑1 (MQ=255)                                                                                             tCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAac                                   >  2:201823/1‑140 (MQ=255)                                                                                             tCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAac                                   >  2:852186/1‑140 (MQ=255)                                                                                             tCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAac                                   >  1:453759/1‑140 (MQ=255)                                                                                             tCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAac                                   >  2:522429/1‑140 (MQ=255)                                                                                             tCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAac                                   >  1:47116/1‑140 (MQ=255)                                                                                             tCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAac                                   >  1:47468/1‑140 (MQ=255)                                                                                              cAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAaca                                  >  2:58950/1‑140 (MQ=255)                                                                                              cAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAaca                                  >  1:934359/1‑140 (MQ=255)                                                                                              cAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAaca                                  <  1:13010/140‑1 (MQ=255)                                                                                                aaTCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACt                                <  1:274946/140‑1 (MQ=255)                                                                                                    gCACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGttt                            >  1:502718/1‑140 (MQ=255)                                                                                                     cACTTCTTTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGtttt                           <  2:1079500/140‑1 (MQ=255)                                                                                                         tcttTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTTTCGGc                       <  1:935480/140‑1 (MQ=255)                                                                                                          cttTTTGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTTTCGGCa                      <  1:1045588/140‑1 (MQ=255)                                                                                                               tGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTTTCGGCATGGCg                 <  1:465305/140‑1 (MQ=255)                                                                                                               tGCAGGCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATACATTATGCCTCTCCCGTGAGCAACACTGTTTTCGGCATGGCg                 <  1:787862/140‑1 (MQ=255)                                                                                                                    gCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCTTCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTTTCGGCATGGCGTagag            <  1:867325/140‑1 (MQ=255)                                                                                                                    gCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTTTCGGCATGGCGTagag            >  2:341925/1‑140 (MQ=255)                                                                                                                    gCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTTTCGGCATGGCGTagag            <  1:564677/140‑1 (MQ=255)                                                                                                                    gCGATCCGCTTCTTCCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTTTCGGCATGGCGTagag            <  1:522429/140‑1 (MQ=255)                                                                                                                              tcttcCGGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTgcg  <  2:37160/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                      GCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCATCAAATCGCACTTCTTTTTGCAGGCGATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTTTAATAGCGATAAATCCATTATGCCTCTCCCGTGAGCAACACTGTTTTCGGCATGGCGTAGAGCACTTCTGCG  >  NC_000913/2505337‑2505601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑