Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	519,790	(G)5→4	coding (58/687 nt)	ybbA →	putative ABC transporter ATP‑binding protein YbbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	519,786	0	G	.	100.0%	336.4 / NA	77	coding (54/687 nt)	ybbA	putative ABC transporter ATP‑binding protein YbbA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (42/35);  total (42/35)

CGGCGCTCAGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTTGATGACGGCAG  >  NC_000913/519648‑519920                                                                                                                                           |                                                                                                                                       cGGCGCTCAGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAgg                                                                                                                                       <  1:900772/140‑1 (MQ=255) cGGCGCTCAGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAgg                                                                                                                                       >  2:736358/1‑140 (MQ=255)  ggCGCTCAGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAggg                                                                                                                                      >  1:1282292/1‑140 (MQ=255)   gcgcTCAGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAgggg                                                                                                                                     >  1:533691/1‑140 (MQ=255)    cgcTCAGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAgggga                                                                                                                                    >  2:1041560/1‑139 (MQ=255)      cTCAGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAggggagc                                                                                                                                  <  1:842558/140‑4 (MQ=255)         aGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTTGGTCA‑GGGGAGCATg                                                                                                                              >  2:309995/1‑140 (MQ=255)         aGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATg                                                                                                                              <  1:1041560/140‑1 (MQ=255)         aGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATg                                                                                                                              <  2:33722/140‑1 (MQ=255)          ggCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGa                                                                                                                             >  2:301255/1‑140 (MQ=255)           gCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGaa                                                                                                                            <  2:1013275/140‑1 (MQ=255)           gCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGaa                                                                                                                            <  1:718705/140‑1 (MQ=255)           gCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGaa                                                                                                                            <  2:1139190/140‑1 (MQ=255)                cACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACtctc                                                                                                                       >  2:631289/1‑140 (MQ=255)                     aGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATcc                                                                                                                  >  2:110341/1‑140 (MQ=255)                         tCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCAc                                                                                                              <  2:346852/140‑1 (MQ=255)                             tAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGa                                                                                                          >  2:270003/1‑140 (MQ=255)                                 gtgtCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTg                                                                                                      >  2:1092109/1‑140 (MQ=255)                                 gtgtCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTg                                                                                                      >  2:372317/1‑140 (MQ=255)                                  tgtCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGa                                                                                                     >  2:184649/1‑140 (MQ=255)                                    tCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTTGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGc                                                                                                   <  1:309995/140‑1 (MQ=255)                                    tCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGc                                                                                                   >  2:648734/1‑140 (MQ=255)                                    tCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGc                                                                                                   <  1:631289/140‑1 (MQ=255)                                     ccGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCt                                                                                                  <  1:28423/140‑1 (MQ=255)                                     ccGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCt                                                                                                  <  1:372317/140‑1 (MQ=255)                                      cGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTg                                                                                                 <  2:533691/140‑1 (MQ=255)                                       gCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTgg                                                                                                <  2:1282292/140‑1 (MQ=255)                                         tGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGtt                                                                                              >  1:247683/1‑140 (MQ=255)                                            ggcggcACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTc                                                                                           >  1:73711/1‑140 (MQ=255)                                            ggcggcACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTc                                                                                           >  2:1016123/1‑140 (MQ=255)                                             gcggcACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCa                                                                                          >  1:789388/1‑140 (MQ=255)                                                          ttAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGAc                                                                             >  2:858268/1‑140 (MQ=255)                                                          ttAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGAc                                                                             <  1:184649/140‑1 (MQ=255)                                                            aaCAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCa                                                                           >  2:335459/1‑140 (MQ=255)                                                            aaCAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCa                                                                           <  2:789388/140‑1 (MQ=255)                                                               aGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCg                                                                        <  1:335459/140‑1 (MQ=255)                                                               aGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCg                                                                        <  1:110341/140‑1 (MQ=255)                                                                ggACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGc                                                                       >  1:792061/1‑140 (MQ=255)                                                                ggACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGc                                                                       >  2:1102528/1‑140 (MQ=255)                                                                 gACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCa                                                                      >  1:211475/1‑140 (MQ=255)                                                                  aCCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCAc                                                                     >  2:316565/1‑140 (MQ=255)                                                                   ccAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACt                                                                    >  1:441976/1‑140 (MQ=255)                                                                    caggaacaggaaGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAAACCATCGCACtg                                                                   >  2:358780/1‑140 (MQ=255)                                                                     aggaacaggaaGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACtgg                                                                  >  2:627333/1‑140 (MQ=255)                                                                       gaacaggaaGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACtggtg                                                                >  2:971560/1‑140 (MQ=255)                                                                         acaggaaGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTggg                                                              <  1:1092109/140‑1 (MQ=255)                                                                         acaggaaGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTggg                                                              <  1:1102528/140‑1 (MQ=255)                                                                            ggaaGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGa                                                           >  1:1357794/1‑140 (MQ=255)                                                                             gaaGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCa                                                                           >  2:1395423/1‑123 (MQ=255)                                                                             gaaGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCa                                                                           <  1:1395423/123‑1 (MQ=255)                                                                             gaaGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAg                                                          >  1:1358639/1‑140 (MQ=255)                                                                              aaGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGt                                                         <  2:1357794/140‑1 (MQ=255)                                                                               aGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGt                                                         <  1:1304736/139‑1 (MQ=255)                                                                               aGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGt                                                         >  2:1304736/1‑139 (MQ=255)                                                                               aGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTc                                                        >  1:11532/1‑140 (MQ=255)                                                                                gggCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCg                                                       <  1:1016123/140‑1 (MQ=255)                                                                                 ggCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCgg                                                      >  2:126698/1‑140 (MQ=255)                                                                                  gCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCggg                                                     >  1:889883/1‑140 (MQ=255)                                                                                   cAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGa                                                    <  2:1358639/140‑1 (MQ=255)                                                                                      aTGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCg                                                 <  1:736358/140‑1 (MQ=255)                                                                                       tGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCgg                                                >  1:908072/1‑140 (MQ=255)                                                                                       tGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCgg                                                <  2:211475/140‑1 (MQ=255)                                                                                            gCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAg                                           >  1:34128/1‑140 (MQ=255)                                                                                                  aaCATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAAcc                                     <  2:1186685/140‑1 (MQ=255)                                                                                                       tgttgAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCt                                >  2:24654/1‑140 (MQ=255)                                                                                                        gttgAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCaa                                       >  1:239391/1‑132 (MQ=255)                                                                                                        gttgAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCaa                                       <  2:239391/132‑1 (MQ=255)                                                                                                         ttgAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGTGAGTCGGGATCGGGTAAGTCAACCTTGCTgg                              >  2:965418/1‑140 (MQ=255)                                                                                                         ttgAAGTTCATCATCTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTgg                              <  1:858268/140‑1 (MQ=255)                                                                                                                   tcatcTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGc                    <  2:792061/140‑1 (MQ=255)                                                                                                                      tcTTAAGAAGTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCgg                 <  1:627333/140‑1 (MQ=255)                                                                                                                           agaagTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTtg            >  1:297036/1‑140 (MQ=255)                                                                                                                            gaagTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTtga           >  1:45533/1‑140 (MQ=255)                                                                                                                             aagTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTtgat          <  2:34128/140‑1 (MQ=255)                                                                                                                             aagTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTtgat          <  2:299792/140‑1 (MQ=255)                                                                                                                             aagTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTtgat          <  2:908072/140‑1 (MQ=255)                                                                                                                              agTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTtg            >  1:126271/1‑137 (MQ=255)                                                                                                                              agTCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTtg            <  2:126271/137‑1 (MQ=255)                                                                                                                                tCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGc                    <  2:448381/127‑1 (MQ=255)                                                                                                                                tCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGc                    >  1:448381/1‑127 (MQ=255)                                                                                                                                tCCGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTTGATGAc       >  1:1036695/1‑140 (MQ=255)                                                                                                                                 ccGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTTGATGACg      >  1:1067646/1‑140 (MQ=255)                                                                                                                                  cGTCGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTTGATGACgg     >  1:90905/1‑140 (MQ=255)                                                                                                                                     cGGTCA‑GGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTTGATGACGgcag  <  2:11532/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                       CGGCGCTCAGGCTATCACCCAGAATCAATAACGTGTCCGCTGCGGCGGCACGGAAGGTTAACAGGACCAGGAACAGGAAGGGCAAATGCCAGCGGAAAACATTGTTGAAGTTCATCATCTTAAGAAGTCCGTCGGTCAGGGGGAGCATGAACTCTCCATCCTCACCGGAGTTGAGCTGGTTGTCAAACGTGGCGAGACCATCGCACTGGTGGGCGAGTCGGGATCGGGTAAGTCAACCTTGCTGGCGATCCTCGCCGGGCTTGATGACGGCAG  >  NC_000913/519648‑519920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑