Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,413,442	(T)7→6	intergenic (‑310/‑28)	yfbV ← / → ackA	UPF0208 membrane protein YfbV/acetate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,413,436	0	T	.	100.0%	403.7 / NA	92	intergenic (‑304/‑34)	yfbV/ackA	UPF0208 membrane protein YfbV/acetate kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (46/46);  total (46/46)

CATTGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCC  >  NC_000913/2413304‑2413571                                                                                                                                     |                                                                                                                                        cATTGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGttttttag                                                                                                                                  <  1:1008625/140‑3 (MQ=255) cATTGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGttttttag                                                                                                                                  <  1:726342/140‑3 (MQ=255)  aTTGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGttttttagc                                                                                                                                 <  1:71180/140‑4 (MQ=255)   ttGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGttttttagcc                                                                                                                                <  2:343266/140‑5 (MQ=255)   ttGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGttttttagcc                                                                                                                                <  2:404231/140‑5 (MQ=255)   ttGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGC‑AAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCa                                                                                                                              >  2:72989/1‑140 (MQ=255)    tGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCa                                                                                                                              >  1:1033495/1‑140 (MQ=255)    tGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCa                                                                                                                              >  1:434746/1‑140 (MQ=255)    tGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCa                                                                                                                              >  1:1251247/1‑140 (MQ=255)    tGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCa                                                                                                                              >  2:1401786/1‑140 (MQ=255)    tGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCa                                                                                                                              >  2:720234/1‑140 (MQ=255)    tGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCa                                                                                                                              >  1:926425/1‑140 (MQ=255)     ggCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCAc                                                                                                                             >  1:551052/1‑140 (MQ=255)     ggCTGAAAATTACGCAAAATGGCATAGACTCAAGATATCTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCAc                                                                                                                             <  1:600930/140‑1 (MQ=255)      gCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACg                                                                                                                            >  1:272734/1‑140 (MQ=255)         gAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTAt                                                                                                                         <  2:789944/140‑1 (MQ=255)         gAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTAt                                                                                                                         <  2:1034425/140‑1 (MQ=255)         gAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTAt                                                                                                                         <  1:429322/140‑1 (MQ=255)         gAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTAt                                                                                                                         <  1:495329/140‑1 (MQ=255)          aaaaTTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATc                                                                                                                        >  1:967591/1‑140 (MQ=255)           aaaTTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCa                                                                                                                       <  2:590091/140‑1 (MQ=255)               tACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATta                                                                                                                   <  1:720234/140‑1 (MQ=255)                aCGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATtat                                                                                                                  <  2:967591/140‑1 (MQ=255)                      aaTGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTAc                                                                                                            >  2:1192096/1‑140 (MQ=255)                             tAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATg                                                                                                     <  2:901137/140‑1 (MQ=255)                                  tCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAg                                                                                                <  2:1358224/140‑1 (MQ=255)                                  tCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAg                                                                                                <  1:905985/140‑1 (MQ=255)                                  tCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAg                                                                                                <  1:670536/140‑1 (MQ=255)                                  tCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAg                                                                                                <  2:1251247/140‑1 (MQ=255)                                  tCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAg                                                                                                <  2:272734/140‑1 (MQ=255)                                  tCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAg                                                                                                <  1:1192096/140‑1 (MQ=255)                                    aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAg                                                                                                <  2:868644/138‑1 (MQ=255)                                    aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAg                                                                                                >  1:868644/1‑138 (MQ=255)                                    aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTa                                                                                              >  1:382525/1‑140 (MQ=255)                                    aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTa                                                                                              >  2:1423654/1‑140 (MQ=255)                                    aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTa                                                                                              <  2:34623/140‑1 (MQ=255)                                    aaGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTa                                                                                              >  2:747862/1‑140 (MQ=255)                                         atTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTa                                                                                         >  2:1283017/1‑140 (MQ=255)                                           ttcttcCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGt                                                                                       >  2:57939/1‑140 (MQ=255)                                             cttcCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTAc                                                                                     <  1:980415/140‑1 (MQ=255)                                             cttcCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTAc                                                                                     <  1:566701/140‑1 (MQ=255)                                             cttcCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTAc                                                                                     <  1:1401786/140‑1 (MQ=255)                                                  atcatGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGtt                                                                                >  2:1402121/1‑140 (MQ=255)                                                    catGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGtt                                                                                <  2:802401/138‑1 (MQ=255)                                                    catGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGtt                                                                                >  1:802401/1‑138 (MQ=255)                                                    catGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCt                                                                              >  2:442221/1‑140 (MQ=255)                                                      tGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGa                                                                            >  1:366047/1‑140 (MQ=255)                                                        cAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAAc                                                                          <  2:1072757/140‑1 (MQ=255)                                                               aaTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTa                                                                   <  1:72989/140‑1 (MQ=255)                                                                aTTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAg                                                                  >  1:142510/1‑140 (MQ=255)                                                                    gCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGttct                                                              <  1:1402121/140‑1 (MQ=255)                                                                              atgTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAt                                                    <  1:57939/140‑1 (MQ=255)                                                                               tgTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAAtt                                                   >  1:714728/1‑140 (MQ=255)                                                                                  tAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGc                                                >  2:274493/1‑140 (MQ=255)                                                                                  tAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGc                                                >  2:1243427/1‑140 (MQ=255)                                                                                    aTCATAAATTTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCca                                              >  1:321901/1‑140 (MQ=255)                                                                                    aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCca                                              >  1:287057/1‑140 (MQ=255)                                                                                    aTCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCca                                              >  1:377605/1‑140 (MQ=255)                                                                                     tCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCcat                                             <  2:382525/140‑1 (MQ=255)                                                                                        tAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCcatcat                                          <  1:274493/140‑1 (MQ=255)                                                                                        tAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCcatcat                                          <  1:276981/140‑1 (MQ=255)                                                                                         aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGcc                                               <  1:1002813/134‑1 (MQ=255)                                                                                         aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGcc                                               >  2:1002813/1‑134 (MQ=255)                                                                                         aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCcatcat                                          >  2:653136/1‑139 (MQ=255)                                                                                         aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCcatcat                                          >  2:1363105/1‑139 (MQ=255)                                                                                         aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCcatcat                                          <  1:1363105/139‑1 (MQ=255)                                                                                         aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCcatcat                                          <  1:653136/139‑1 (MQ=255)                                                                                         aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCatcatc                                         >  2:1193619/1‑140 (MQ=255)                                                                                         aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCatcatc                                         >  1:197254/1‑140 (MQ=255)                                                                                         aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCatcatc                                         >  2:578173/1‑140 (MQ=255)                                                                                         aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCatcatc                                         >  1:1421349/1‑140 (MQ=255)                                                                                         aaaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCatcatc                                         >  1:658894/1‑140 (MQ=255)                                                                                          aaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCg                                        <  1:1029821/140‑1 (MQ=255)                                                                                          aaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCg                                        <  2:1421349/140‑1 (MQ=255)                                                                                          aaTGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCg                                        <  1:578173/140‑1 (MQ=255)                                                                                            tGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGAt                                      <  1:747862/140‑1 (MQ=255)                                                                                             gTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATg                                     <  2:434746/140‑1 (MQ=255)                                                                                               cGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCa                                   <  1:72217/140‑1 (MQ=255)                                                                                                ggTGTCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAg                                  >  2:632965/1‑140 (MQ=255)                                                                                                 gtgtCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGt                                 <  2:667258/140‑1 (MQ=255)                                                                                                 gtgtCATCATGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGt                                 <  2:714728/140‑1 (MQ=255)                                                                                                    tcatcaTGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTaaa                              <  2:787728/140‑1 (MQ=255)                                                                                                    tcatcaTGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTaaa                              <  2:551052/140‑1 (MQ=255)                                                                                                        catGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGt                          <  2:377605/140‑1 (MQ=255)                                                                                                        catGCGCTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGt                          <  2:197254/140‑1 (MQ=255)                                                                                                           gcgcTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGaa                       <  1:1283017/140‑1 (MQ=255)                                                                                                              cTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAagag                    >  1:478171/1‑140 (MQ=255)                                                                                                              cTACGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAagag                    >  1:996894/1‑140 (MQ=255)                                                                                                                aCGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTa                  <  2:1033495/140‑1 (MQ=255)                                                                                                                 cGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGt                          >  2:1227816/1‑131 (MQ=255)                                                                                                                 cGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGt                          <  1:1227816/131‑1 (MQ=255)                                                                                                                 cGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTAc                 >  2:1410841/1‑140 (MQ=255)                                                                                                                 cGCTCTATGGCTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTAc                 >  2:996936/1‑140 (MQ=255)                                                                                                                           cTCCCTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGtt       >  1:201091/1‑140 (MQ=255)                                                                                                                             cccTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTa     >  2:300802/1‑140 (MQ=255)                                                                                                                              ccTGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAg    >  1:481192/1‑140 (MQ=255)                                                                                                                                tGACG‑TTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGcc  >  2:316444/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                        CATTGGCTGAAAATTACGCAAAATGGCATAGACTCAAGATATTTCTTCCATCATGCAAAAAAAATTTGCAGTGCATGATGTTAATCATAAATGTCGGTGTCATCATGCGCTACGCTCTATGGCTCCCTGACGTTTTTTTAGCCACGTATCAATTATAGGTACTTCCATGTCGAGTAAGTTAGTACTGGTTCTGAACTGCGGTAGTTCTTCACTGAAATTTGCCATCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCC  >  NC_000913/2413304‑2413571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑