Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,330	(G)8→9	noncoding (72/87 nt)	leuQ ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,322	1	.	G	92.7%	173.6 / 6.7	55	noncoding (80/87 nt)	leuQ	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (2/2);  new base G (26/25);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

TTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTT  >  NC_000913/4606183‑4606456                                                                                                                                             |                                                                                                                                         tttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                                                                                                           <  1:1201300/140‑1 (MQ=255)  ttGATATCGCCTCGTTTGGTGCGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCGCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑g                                                                                                                                         <  1:120250/140‑1 (MQ=255)  ttGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑g                                                                                                                                         <  1:620324/140‑1 (MQ=255)      tatCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gg‑ggg                                                                                                                                    <  1:512869/140‑1 (MQ=255)       atCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gg‑gggg                                                                                                                                   <  1:186889/140‑1 (MQ=255)         cGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gg‑gggg‑gg                                                                                                                                <  2:121229/140‑1 (MQ=255)         cGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gg‑gggg‑gg                                                                                                                                <  1:1143778/140‑1 (MQ=255)          gCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gg‑gggg‑ggg                                                                                                                               <  2:1071497/140‑2 (MQ=255)                tttGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gg‑gggg‑ggggccttg                                                                                                                         >  1:644029/1‑133 (MQ=255)                   ggTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGTGAGGG‑GGGG‑GGACTTGAAcc                                                                                                                       <  2:317276/140‑1 (MQ=255)                   ggTGAGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAAcc                                                                                                                       <  2:1369224/140‑1 (MQ=255)                    gTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAAccc                                                                                                                      <  2:562695/140‑1 (MQ=255)                     tGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAAcccc                                                                                                                     <  1:169645/140‑1 (MQ=255)                       cGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCa                                                                                                                   <  1:912046/140‑1 (MQ=255)                         aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACg                                                                                                                 <  1:684195/140‑1 (MQ=255)                         aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACg                                                                                                                 <  2:42980/140‑1 (MQ=255)                          ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gg‑gggg‑g                                                                                                                                 <  1:859328/122‑1 (MQ=255)                          ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑gg‑gggg‑g                                                                                                                                 >  2:859328/1‑122 (MQ=255)                              ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGt                                                                                                            <  2:1037830/140‑1 (MQ=255)                               gggACTTGAACCCCCCCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTa                                                                                                           <  2:110057/140‑1 (MQ=255)                               gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTa                                                                                                           <  2:765950/140‑1 (MQ=255)                               gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTa                                                                                                           >  2:235674/1‑140 (MQ=255)                               gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTa                                                                                                           >  1:334525/1‑140 (MQ=255)                               gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTa                                                                                                           <  1:1424653/140‑1 (MQ=255)                                   cTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGa                                                                                                       >  2:1069586/1‑140 (MQ=255)                                     tGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGaca                                                                                                     <  1:235674/140‑1 (MQ=255)                                         cccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                                 <  1:681890/140‑1 (MQ=255)                                            ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGCGAGCAATATCAACGTGGTTTTTGCTGCGAGGG‑GGGG‑GGTCTTGAACCCCCACTTCCGTAAAGACACTAacac                                                                                              >  2:1363157/1‑140 (MQ=25)                                                    gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                                                      >  1:278460/1‑140 (MQ=255)                                                           cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GGCGGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGACGCTAGcgcgc                                                                               >  1:378562/1‑139 (MQ=255)                                                           cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCACCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                                                                               >  2:1346763/1‑140 (MQ=255)                                                            acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGTGCGA‑GG‑GGGGTGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                                                              >  2:23552/1‑140 (MQ=255)                                                             cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                                             >  1:1100998/1‑140 (MQ=255)                                                              tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                            >  2:1359677/1‑140 (MQ=255)                                                              tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                                                            >  1:1239048/1‑140 (MQ=255)                                                                   ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                       >  1:1227125/1‑140 (MQ=255)                                                                      gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                    >  1:1023642/1‑140 (MQ=255)                                                                      gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                    >  1:1219984/1‑140 (MQ=255)                                                                        aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                                                                 >  2:555364/1‑140 (MQ=255)                                                                        aGCTAGCGCGTCTACCAATTCCACCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  1:1346763/140‑1 (MQ=255)                                                                          cTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACCAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                <  1:1069586/140‑1 (MQ=255)                                                                            aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                              >  2:205415/1‑140 (MQ=255)                                                                              cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                            >  1:407173/1‑140 (MQ=255)                                                                              cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                            >  2:901426/1‑140 (MQ=255)                                                                                cgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                                                          <  1:205415/140‑1 (MQ=255)                                                                                   cTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt                                                       <  1:1361695/140‑1 (MQ=255)                                                                                                   cTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaa                                       >  1:340641/1‑140 (MQ=255)                                                                                                      cGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAg                                    <  2:1023642/140‑1 (MQ=255)                                                                                                      cGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAg                                    <  2:1219984/140‑1 (MQ=255)                                                                                                             tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                                  >  1:431498/1‑135 (MQ=255)                                                                                                             tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                                  <  2:431498/135‑1 (MQ=255)                                                                                                             tGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCt                             >  1:966455/1‑140 (MQ=255)                                                                                                              gCGAGCAATATCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTa                            <  2:1239048/140‑1 (MQ=255)                                                                                                                   cAATATCATCGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAAGCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                       <  2:378562/140‑1 (MQ=255)                                                                                                                     atatCAACGTGGTTTTTGGTGCGA‑GG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                    <  2:912205/140‑1 (MQ=255)                                                                                                                      tatCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                    >  1:1325457/1‑140 (MQ=255)                                                                                                                      tatCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                    <  2:278460/140‑1 (MQ=255)                                                                                                                      taccAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                    >  1:1055647/4‑140 (MQ=255)                                                                                                                       atCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAAccccc                                                                                                                    <  2:1153709/43‑1 (MQ=21)                                                                                                                       atCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAAccccc                                                                                                                    >  1:1153709/1‑43 (MQ=21)                                                                                                                       atCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg                   >  2:1158501/1‑140 (MQ=255)                                                                                                                       atCAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg                   >  2:998683/1‑140 (MQ=255)                                                                                                                         cAACGTGGTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                 <  2:1100998/140‑1 (MQ=255)                                                                                                                          aaCGTGGTTTTTGGTGCGA‑GG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCa               <  1:555364/140‑1 (MQ=255)                                                                                                                                gTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa          >  2:85089/1‑140 (MQ=255)                                                                                                                                gTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa          >  1:485055/1‑140 (MQ=255)                                                                                                                                gTTTTTGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa          >  1:298827/1‑140 (MQ=255)                                                                                                                                 tttttGGTGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg         >  1:979343/1‑140 (MQ=255)                                                                                                                                        tGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTCACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGtttt  <  2:1325457/140‑1 (MQ=255)                                                                                                                                        tGCGAGGG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGtttt  <  2:1055647/140‑1 (MQ=255)                                                                                                                                             |                                                                                                                                         TTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GG‑GGGG‑GGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTT  >  NC_000913/4606183‑4606456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑