Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,351,196	(G)7→9	intergenic (‑157/+211)	fabI ← / ← ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,351,189	1	.	G	97.0%	232.2 / ‑0.1	68	intergenic (‑150/+218)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base G (29/36);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01
*	NC_000913	1,351,189	2	.	G	97.1%	235.9 / ‑0.1	68	intergenic (‑150/+218)	fabI/ycjD	enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base G (30/36);  total (32/36)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

TTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351051‑1351319                                                                                                                                            ||                                                                                                                                     ttATTGTTGATGCTTGCTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑g                                                                                                                                     >  2:1106204/1‑140 (MQ=255)  tATTGTTGATGCTTGCTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑gg                                                                                                                                    >  2:233998/1‑140 (MQ=255)  tATTGTTGATGCTTGCTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑gg                                                                                                                                    >  2:267479/1‑140 (MQ=255)         gATGCTTGCTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑ggggggggg                                                                                                                             >  1:926304/1‑138 (MQ=255)          aTGCTTGCTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGgggc                                                                                                                            >  1:113911/1‑140 (MQ=255)          aTGCTTGCTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGgggc                                                                                                                            >  2:703386/1‑140 (MQ=255)                    tgCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTcccc                                                                                                                   <  2:113911/140‑1 (MQ=255)                     gCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGCGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCt                                                                                                                  <  2:1266216/140‑1 (MQ=255)                     gCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTccccc                                                                                                                  >  2:1007576/1‑139 (MQ=255)                      ccTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTc                                                                                                                 >  1:239453/1‑140 (MQ=255)                          aaaaTCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCAccc                                                                                                             >  2:442904/1‑140 (MQ=255)                              tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGgggcgcttt                                                                                                                       >  1:829031/1‑120 (MQ=255)                               cAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAAcc                                                                                                        <  1:1365055/140‑1 (MQ=255)                               cAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAAcc                                                                                                        <  1:267479/140‑1 (MQ=255)                               cAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAAcc                                                                                                        <  1:703386/140‑1 (MQ=255)                                 ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCct                                                                                                      >  1:621734/1‑140 (MQ=255)                                        cgtcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCa                                                                                               <  2:465954/140‑1 (MQ=255)                                          tcgtTTTAGTAAACAGTACGAACAGATAAACGGTTCTTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCTGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                             <  2:983834/140‑1 (MQ=255)                                          tcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                             <  1:1249278/140‑1 (MQ=255)                                               ttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGc                                                                                        <  1:442904/140‑1 (MQ=255)                                                 aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                      <  2:1343125/140‑1 (MQ=255)                                                 aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                      <  2:621734/140‑1 (MQ=255)                                                     aaCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgggg                                                                                  >  1:1281133/1‑140 (MQ=255)                                                      aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                 >  1:532442/1‑140 (MQ=255)                                                          tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGt                                                                             >  2:15156/1‑140 (MQ=255)                                                          tACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGt                                                                             >  1:835557/1‑140 (MQ=255)                                                           aCGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                            <  1:503411/140‑1 (MQ=255)                                                            cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAt                                                                           <  1:701583/140‑1 (MQ=255)                                                              aaCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtg                                                                         <  2:926304/140‑1 (MQ=255)                                                               aCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgt                                                                        >  2:1317540/1‑140 (MQ=255)                                                                   aTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaa                                                                    >  1:1027072/1‑140 (MQ=255)                                                                    tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                       <  1:403788/136‑1 (MQ=255)                                                                    tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATtgtg                                                                       >  2:403788/1‑136 (MQ=255)                                                                    tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                   >  2:977629/1‑140 (MQ=255)                                                                    tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCaaa                                                                   >  1:601900/1‑140 (MQ=255)                                                                    tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTCCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCCGAGGGGGGAGGGGAGCGTATTGTGCaaa                                                                   >  2:1025352/1‑140 (MQ=38)                                                                     aaaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAat                                                                  <  2:239453/140‑1 (MQ=255)                                                                           ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCATGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCCGAGGGGCGAGGGGACCGGATTGTGCAAATATTGtt                                                            >  1:426330/1‑140 (MQ=38)                                                                           ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGtt                                                            >  2:766531/1‑140 (MQ=255)                                                                           ttattaTAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGtt                                                            <  2:1281133/140‑1 (MQ=255)                                                                             attatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAc                                                          >  2:1126611/1‑140 (MQ=255)                                                                                   aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑c                                                   >  2:634861/1‑140 (MQ=255)                                                                                   aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑c                                                   >  2:1073645/1‑140 (MQ=255)                                                                                        ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑caacaa                                              >  2:217448/1‑140 (MQ=255)                                                                                        ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑caacaa                                              >  1:648144/1‑140 (MQ=255)                                                                                        ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑caacaa                                              >  2:38588/1‑140 (MQ=255)                                                                                        ccTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCT‑CCCTGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑Caacaaa                                             <  2:17912/140‑1 (MQ=39)                                                                                         caggCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑Caacaaa                                             <  1:716423/138‑1 (MQ=255)                                                                                         cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTTCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑Caacaaa                                             >  2:563572/1‑140 (MQ=255)                                                                                         cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑Caacaaa                                             <  1:15156/140‑1 (MQ=255)                                                                                         cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑Caacaaa                                             <  2:648144/140‑1 (MQ=255)                                                                                         cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑Caacaaa                                             <  1:217448/140‑1 (MQ=255)                                                                                         cTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑Caacaaa                                             <  1:1126611/140‑1 (MQ=255)                                                                                             cTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAgg                                         <  2:1027072/140‑1 (MQ=255)                                                                                               gtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAgg                                         <  2:128874/138‑1 (MQ=255)                                                                                               gtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAgg                                         >  1:128874/1‑138 (MQ=255)                                                                                                  aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCAt                                    >  2:160419/1‑140 (MQ=255)                                                                                                       cTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCCCCCCAACCCTCTCCCCAGAGGGGCGGGGGGGCCGTATTGTGCAAATATTTTTAACCCCG‑CAAAAAACAAGCTCATAcctg                               >  2:716423/1‑137 (MQ=37)                                                                                                           aGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCt                           <  2:1197016/140‑1 (MQ=255)                                                                                                            gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTa                          <  1:825252/140‑1 (MQ=255)                                                                                                              tGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAAc                        <  1:766531/140‑1 (MQ=255)                                                                                                               gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGCTTTTTCCCCCCCCCCAAACCCCCCCCCCAAAGGGGGGGGGGGCCCG‑ATTTTGGAAAAATTTTTTCCCCCGCCAAAAAAAAGGC‑CATAAAACCCCCaaac                       >  2:1416808/1‑138 (MQ=255)                                                                                                               gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATa                                   >  2:913894/1‑128 (MQ=255)                                                                                                               gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATa                                   <  1:913894/128‑1 (MQ=255)                                                                                                               gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAAcc                       >  2:1248276/1‑140 (MQ=255)                                                                                                                ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGGGC‑TTTTCCCCCCCCCCCAACCCCCCCCCCCCAGGGGGGGGGGGGCCGGATTTTGGAAAAATTTTTTTCCCCC‑CAACAAAAAAGGCCATACAACCCCCCAccc                      >  2:180604/1‑140 (MQ=255)                                                                                                                 cAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAACCCt                     >  1:60614/1‑140 (MQ=255)                                                                                                                 cAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCAACCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAACCCt                     >  1:945394/1‑140 (MQ=255)                                                                                                                          ccGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg            <  1:1106204/140‑1 (MQ=255)                                                                                                                          ccGGAGCAGGCTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCg            <  1:634861/140‑1 (MQ=255)                                                                                                                                    cTGCGGCAGGGGGGGGGGGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  1:180604/140‑1 (MQ=255)                                                                                                                                    cTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  2:835557/140‑1 (MQ=255)                                                                                                                                    cTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  2:60614/140‑1 (MQ=255)                                                                                                                                    cTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  1:1248276/140‑1 (MQ=255)                                                                                                                                    cTGCGGCAGGGGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGa  <  2:532442/140‑1 (MQ=255)                                                                                                                                            ||                                                                                                                                     TTATTGTTGATGCTTGTTGTGCCTGAAAATCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCA‑‑GGGGGGGCGC‑TTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAG‑CAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGA  >  NC_000913/1351051‑1351319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑