Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,571,276	+G	coding (476/1404 nt)	eutE ←	putative aldehyde dehydrogenase, ethanolamine utilization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,571,272	1	.	G	100.0%	179.7 / NA	51	P160P (CCG→CCC)	eutE	putative aldehyde dehydrogenase, ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (21/30);  total (21/30)

AAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGC‑GGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCG  >  NC_000913/2571134‑2571404                                                                                                                                            |                                                                                                                                     aaaCTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGC‑g                                                                                                                                     <  2:37953/140‑1 (MQ=255) aaaCTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGC‑g                                                                                                                                     <  2:1007393/140‑1 (MQ=255)             gCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATg                                                                                                                          >  2:637455/1‑140 (MQ=255)              cGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGa                                                                                                                         >  1:652734/1‑140 (MQ=255)              cGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGa                                                                                                                         <  1:744108/140‑1 (MQ=255)                   cgcgGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGTAGACTTTTTTCGCCGCCGGATGC‑ggggg                                                                                                                                 >  2:1334089/1‑125 (MQ=255)                   cgcgGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTg                                                                                                                    <  2:1201636/140‑1 (MQ=255)                   cgcgGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGC‑ggggg                                                                                                                                 <  1:1334089/126‑2 (MQ=255)                           cGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCcgc                                                                                                            <  1:637455/140‑1 (MQ=255)                           cGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCcgc                                                                                                            <  1:711659/140‑1 (MQ=255)                                 ccGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAAt                                                                                                      <  1:78662/140‑1 (MQ=255)                                  cGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATc                                                                                                     >  2:992936/1‑140 (MQ=255)                                    gATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAg                                                                                                   >  2:544893/1‑140 (MQ=255)                                    gATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAg                                                                                                   >  2:895431/1‑140 (MQ=255)                                      tttGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGc                                                                                                 <  1:992936/140‑1 (MQ=255)                                        tGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTg                                                                                               >  1:1278386/1‑140 (MQ=255)                                         gCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGa                                                                                              <  2:1319498/140‑1 (MQ=255)                                         gCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGa                                                                                              <  1:1109569/140‑1 (MQ=255)                                         gCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGa                                                                                              <  1:388951/140‑1 (MQ=255)                                               gTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCgt                                                                                        <  1:305166/140‑1 (MQ=255)                                               gTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTAATGGCgt                                                                                        >  2:1411121/1‑140 (MQ=255)                                                   ccAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCgttgtt                                                                                    >  2:840027/1‑140 (MQ=255)                                                    cAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTa                                                                                   >  1:855524/1‑140 (MQ=255)                                                    cAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTa                                                                                   >  1:535199/1‑140 (MQ=255)                                                          gTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCgtt                                                                                       <  1:578413/130‑1 (MQ=255)                                                          gTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCgtt                                                                                       >  2:578413/1‑130 (MQ=255)                                                            tttCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGt                                                                           <  2:855524/140‑1 (MQ=255)                                                                  gCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTCCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTgccgc                                                                     <  2:1278386/140‑1 (MQ=255)                                                                    ccACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGccgccg                                                                   <  2:252127/140‑1 (MQ=255)                                                                            ggCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTg                                                           >  1:542264/1‑140 (MQ=255)                                                                                acaaTCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAg                                                       >  2:979155/1‑140 (MQ=255)                                                                                     cGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTc                                                  <  2:535199/140‑1 (MQ=255)                                                                                      gCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCa                                                 <  2:997031/140‑1 (MQ=255)                                                                                       ccTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGTGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCAc                                                <  2:652734/140‑1 (MQ=255)                                                                                       ccTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCAc                                                <  1:979155/140‑1 (MQ=255)                                                                                            ttGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAg                                           <  1:337070/140‑1 (MQ=255)                                                                                                gcagcGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGccac                                       >  2:158166/1‑140 (MQ=255)                                                                                                 cagcGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGccacc                                      <  1:840027/140‑1 (MQ=255)                                                                                                 cagcGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGccacc                                      <  1:544893/140‑1 (MQ=255)                                                                                                 cagcGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGccacc                                      <  1:1239464/140‑1 (MQ=255)                                                                                                 cagcGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGccacc                                      <  1:54612/140‑1 (MQ=255)                                                                                                 cagcGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGccacc                                      <  2:542264/140‑1 (MQ=255)                                                                                                 cagcGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGccacc                                      >  2:1364732/1‑140 (MQ=255)                                                                                                 cagcGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGccacc                                      <  1:158166/140‑1 (MQ=255)                                                                                                      tAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGccacc                                      >  2:67815/1‑135 (MQ=255)                                                                                                      tAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGccacc                                      <  1:67815/135‑1 (MQ=255)                                                                                                      tAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGc                                          >  2:1063693/1‑131 (MQ=255)                                                                                                      tAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGc                                          <  1:1063693/131‑1 (MQ=255)                                                                                                      tAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGcc                                 >  2:907754/1‑140 (MQ=255)                                                                                                              cGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTg                         >  1:861605/1‑140 (MQ=255)                                                                                                              cGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTg                         >  2:844995/1‑140 (MQ=255)                                                                                                              cGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGAGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTg                         >  1:1212635/1‑140 (MQ=255)                                                                                                              cGCTGGGAGACTTTTTTCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTAATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTg                         <  1:1411121/140‑1 (MQ=255)                                                                                                                              tCGCCGCCGGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGt         <  1:907754/140‑1 (MQ=255)                                                                                                                                     cgGATGCGGGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGAGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCg  <  2:1212635/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                     AAACTTGAACAAGCGTTGCGCGGTTTCGATATCCGGATTTGCCACAGTAACCAGTAAGTTTTCCGGCCCACCTGCGGCAACAATCGCCTGGTTGAGCAGCGTAATCGCCCGCTGGGAGACTTTTTTCGCCGCCGGATGC‑GGGGCAAAAATGACGCTGTTGCCCGCGGCAATCAGGCTGATGGCGTTGTTAATTACGGTTGCCGCCGGGTTAGTGGAAGGCGTCACCGAAGCCACCACGCCCCAGGGTGCGTTTTCAATTAGGGTCAGGCCG  >  NC_000913/2571134‑2571404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑