Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	324,226	A→G	S157G (AGC→GGC)	ykgG →	DUF162 domain‑containing lactate utilization protein YkgG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	324,226	0	A	G	100.0%	185.4 / NA	52	S157G (AGC→GGC)	ykgG	DUF162 domain‑containing lactate utilization protein YkgG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/32);  total (20/32)

TGGGATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAA  >  NC_000913/324091‑324350                                                                                                                                        |                                                                                                                             tGGGATCCGGCGAAAGGTGCCGAGAATATCTCGCATGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCt                                                                                                                          <  2:984762/140‑1 (MQ=255) tGGGATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCt                                                                                                                          <  1:67457/140‑1 (MQ=255) tGGGATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCt                                                                                                                          >  2:803291/1‑140 (MQ=255)     aTCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTc                                                                                                                      <  1:1129524/140‑1 (MQ=255)       ccGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTccc                                                                                                                    <  2:309744/140‑1 (MQ=255)          gCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGa                                                                                                                 <  1:1087634/140‑1 (MQ=255)                ggTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATtc                                                                                                           <  1:564296/140‑1 (MQ=255)                ggTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATtc                                                                                                           <  1:803291/140‑1 (MQ=255)                ggTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATtc                                                                                                           <  1:120590/140‑1 (MQ=255)                ggTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATtc                                                                                                           >  2:1001335/1‑140 (MQ=255)                ggTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATtc                                                                                                           <  1:1112796/140‑1 (MQ=255)                    ccGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCtt                                                                                                       >  1:1392036/1‑140 (MQ=255)                          atatCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATc                                                                                                 <  2:342053/140‑1 (MQ=255)                          atatCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATc                                                                                                 <  1:716799/140‑1 (MQ=255)                                  caggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaa                                                                                         <  2:1084612/140‑1 (MQ=255)                                  caggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaa                                                                                         <  1:1153007/140‑1 (MQ=255)                                   aggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaa                                                                                        >  2:791781/1‑140 (MQ=255)                                   aggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaa                                                                                        >  2:864595/1‑140 (MQ=255)                                   aggcagAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTaaa                                                                                        >  1:358625/1‑140 (MQ=255)                                       agagCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCa                                                                                    <  1:469399/140‑1 (MQ=255)                                       agagCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCa                                                                                    <  2:867338/140‑1 (MQ=255)                                            aGGCTAAAGTGGGTGTTGTGTATGCTCAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATc                                                                               <  1:1001335/140‑1 (MQ=255)                                               cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAg                                                                                      <  2:117266/130‑1 (MQ=255)                                               cTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAg                                                                                      >  1:117266/1‑130 (MQ=255)                                               cTAAAGTGGGTGTTGTGTATGCTGAATATGGATTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTg                                                                            >  2:727502/1‑140 (MQ=255)                                                 aaaGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGcc                                                                          <  2:1131658/140‑1 (MQ=255)                                                 aaaGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGcc                                                                          <  1:67285/140‑1 (MQ=255)                                                 aaaGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGcc                                                                          <  2:1392036/140‑1 (MQ=255)                                                        gtgtTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCa                                                                                    >  1:998238/1‑123 (MQ=255)                                                        gtgtTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCa                                                                                    <  2:998238/123‑1 (MQ=255)                                                        gtgtTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTa                                                                   >  2:755097/1‑140 (MQ=255)                                                               gtATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAAc                                                            >  2:936677/1‑140 (MQ=255)                                                                tATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACt                                                           <  2:909332/140‑1 (MQ=255)                                                                        atatGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGaaa                                                   >  1:551714/1‑140 (MQ=255)                                                                        atatGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGaaa                                                   <  2:219508/140‑1 (MQ=255)                                                                          atGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGaaaaa                                                 >  2:1318079/1‑140 (MQ=255)                                                                              tttAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGc                                             >  2:1039643/1‑140 (MQ=255)                                                                                    cGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGa                                       <  1:1318079/140‑1 (MQ=255)                                                                                      aaTCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGaaa                                     >  1:459891/1‑140 (MQ=255)                                                                                           ggAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCa                                >  1:743180/1‑140 (MQ=255)                                                                                            gAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGTCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAg                               >  1:1150375/1‑140 (MQ=255)                                                                                                cGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCg                           <  1:1039643/140‑1 (MQ=255)                                                                                                  tGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGt                         >  2:1180787/1‑140 (MQ=255)                                                                                                      tCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAAc                     <  1:936677/140‑1 (MQ=255)                                                                                                        ttttttCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGa                   <  2:459891/140‑1 (MQ=255)                                                                                                           tttCCGCCGCCGAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATg                <  2:743180/140‑1 (MQ=255)                                                                                                              ccgccgccgAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCt             >  2:1071055/1‑140 (MQ=255)                                                                                                               cgccgccgAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCtt            >  2:543463/1‑140 (MQ=255)                                                                                                                gccgccgAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTc           <  2:389650/140‑1 (MQ=255)                                                                                                                gccgccgAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTc           <  2:551714/140‑1 (MQ=255)                                                                                                                 ccgccgAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTcc          >  1:1347786/1‑140 (MQ=255)                                                                                                                 ccgccgAGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTcc          <  2:824716/140‑1 (MQ=255)                                                                                                                       aGCGCGGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCAtt    <  1:755097/140‑1 (MQ=255)                                                                                                                         cgcgGGCGTTCATTGGGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTaa  <  1:1180787/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                             TGGGATCCGGCGAAAGGTGCCGAGAATATCTCGCAGGCAGAGCAGGCTAAAGTGGGTGTTGTGTATGCTGAATATGGTTTAACCGAATCGGGAGGCGTGGTTCTTTTTTCCGCCGCCGAGCGCGGGCGTTCATTGAGCCTGCTCCCGGAATATTCTCTTTTTATCCTGCGTAAAAGCACTATCCTGCCGCGTGTAGCGCAACTCGCAGAAAAATTGCATCAGAAAGCGCAGGCCGGTGAACGAATGCCTTCCTGCATTAA  >  NC_000913/324091‑324350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑