Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,195,947	G→A	V269V (GTC→GTT)	rfaE ←	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,195,947	0	G	A	100.0%	306.3 / NA	86	V269V (GTC→GTT)	rfaE	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/47);  total (39/47)

TCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGA  >  NC_000913/3195812‑3196078                                                                                                                                        |                                                                                                                                    tCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGc                                                                                                                                 >  2:955664/1‑140 (MQ=255) tCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGc                                                                                                                                 >  1:564610/1‑140 (MQ=255) tCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGc                                                                                                                                 >  2:1274018/1‑140 (MQ=255) tCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGc                                                                                                                                 >  2:552296/1‑140 (MQ=255) tCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGc                                                                                                                                 >  2:647523/1‑140 (MQ=255)  ccACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGcc                                                                                                                                <  1:955664/140‑1 (MQ=255)   cACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGCGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCa                                                                                                                               >  1:1134578/1‑140 (MQ=255)     cGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAAt                                                                                                                             <  1:123393/140‑1 (MQ=255)          aGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCg                                                                                                                        >  2:450898/1‑140 (MQ=255)            cATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCgtg                                                                                                                      >  2:229411/1‑140 (MQ=255)            cATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCgtg                                                                                                                      >  1:686090/1‑140 (MQ=255)            cATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCgtg                                                                                                                      >  1:196177/1‑140 (MQ=255)            cATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCgtg                                                                                                                      >  2:654374/1‑140 (MQ=255)            cATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCgtg                                                                                                                      >  2:671040/1‑140 (MQ=255)              ttttCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTc                                                                                                                    >  1:566640/1‑140 (MQ=255)              ttttCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTc                                                                                                                    >  2:1204893/1‑140 (MQ=255)              ttttCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTc                                                                                                                    >  2:749773/1‑140 (MQ=255)               tttCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCg                                                                                                                   >  2:38266/1‑140 (MQ=255)                 tCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGcc                                                                                                                 <  2:212942/140‑1 (MQ=255)                   cAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCg                                                                                                               <  1:975527/140‑1 (MQ=255)                    aGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGc                                                                                                              <  1:405212/140‑1 (MQ=255)                    aGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGc                                                                                                              <  1:749773/140‑1 (MQ=255)                      cTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGc                                                                                                              >  1:1406234/1‑138 (MQ=255)                      cTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGc                                                                                                              <  2:1406234/138‑1 (MQ=255)                      cTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCAc                                                                                                            >  1:219165/1‑140 (MQ=255)                         gATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCgg                                                                                                         <  2:564610/140‑1 (MQ=255)                         gATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCACCAGAACGCCAATCACCGTGTCGCCCGCACCgg                                                                                                         <  1:819399/140‑1 (MQ=255)                          aTCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGt                                                                                                        >  2:468237/1‑140 (MQ=255)                           tCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTa                                                                                                       <  1:468237/140‑1 (MQ=255)                           tCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTa                                                                                                       <  1:586422/140‑1 (MQ=255)                           tCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTa                                                                                                       <  1:552296/140‑1 (MQ=255)                           tCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTa                                                                                                       <  1:450898/140‑1 (MQ=255)                           tCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTa                                                                                                       >  2:556150/1‑140 (MQ=255)                           tCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTa                                                                                                       <  1:1274018/140‑1 (MQ=255)                            cGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTaa                                                                                                      <  1:1056272/140‑1 (MQ=255)                                 aaaCCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCa                                                                                                 <  1:671040/140‑1 (MQ=255)                                   aCCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATa                                                                                               <  2:45485/140‑1 (MQ=255)                                        ggAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACtt                                                                                          >  1:556377/1‑140 (MQ=255)                                         gAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTc                                                                                         <  2:1088246/140‑1 (MQ=255)                                              tCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTgcg                                                                                    >  2:1257588/1‑140 (MQ=255)                                               cccAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTgcgc                                                                                   <  2:686090/140‑1 (MQ=255)                                               cccAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTgcgc                                                                                   <  2:219165/140‑1 (MQ=255)                                               cccAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTgcgc                                                                                   <  1:47548/140‑1 (MQ=255)                                               cccAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTgcgc                                                                                   >  2:1186115/1‑140 (MQ=255)                                                   gTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTgg                                                                               >  2:1211114/1‑140 (MQ=255)                                                    tttGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTggg                                                                              <  2:566640/140‑1 (MQ=255)                                                    tttGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTggg                                                                              <  1:43045/140‑1 (MQ=255)                                                    tttGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTggg                                                                              <  2:467722/140‑1 (MQ=255)                                                    tttGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTggg                                                                              <  1:556150/140‑1 (MQ=255)                                                      tGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGtt                                                                            >  2:517355/1‑140 (MQ=255)                                                      tGCCAACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGtt                                                                            >  2:1172061/1‑140 (MQ=255)                                                                  cGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAgc                                                                >  2:637351/1‑140 (MQ=255)                                                                       gccgccGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGGGCTTGGGTTGGCATATGCAGCGgcgc                                                           >  1:999825/1‑140 (MQ=255)                                                                       gccgccGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGgcgc                                                           >  2:621216/1‑140 (MQ=255)                                                                       gccgccGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGgcgc                                                           >  2:1324928/1‑140 (MQ=255)                                                                           ccgcATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTa                                                       <  1:731813/140‑1 (MQ=255)                                                                                   gCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTg                                               <  1:914391/140‑1 (MQ=255)                                                                                      aagaagCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTgcag                                            <  1:637351/140‑1 (MQ=255)                                                                                        gaagCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTgcggca                                          <  1:647523/140‑1 (MQ=255)                                                                                        gaagCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTgcagca                                          <  2:196177/140‑1 (MQ=255)                                                                                         aagCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGcagcag                                         <  2:556377/140‑1 (MQ=255)                                                                                          agCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCagcagc                                        <  1:1204893/140‑1 (MQ=255)                                                                                           gCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCg                                       <  1:1060855/140‑1 (MQ=255)                                                                                             aGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGAc                                     >  1:753114/1‑140 (MQ=255)                                                                                             aGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGAc                                     >  1:1031800/1‑140 (MQ=255)                                                                                              ggCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACa                                    >  1:153185/1‑140 (MQ=255)                                                                                                  tcttcCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATAcc                                <  2:438366/140‑1 (MQ=255)                                                                                                  tcttcCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATAcc                                <  1:331254/140‑1 (MQ=255)                                                                                                   cttcCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATAccc                               >  2:192434/1‑140 (MQ=255)                                                                                                   cttcCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATAccc                               >  2:77046/1‑140 (MQ=255)                                                                                                         gCGAATTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCg                         >  2:284810/1‑140 (MQ=255)                                                                                                            aaTTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGaa                      <  2:880360/140‑1 (MQ=255)                                                                                                            aaTTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGaa                      <  2:632395/140‑1 (MQ=255)                                                                                                             aTTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAAc                     <  1:192434/140‑1 (MQ=255)                                                                                                             aTTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAAc                     <  2:753114/140‑1 (MQ=255)                                                                                                             aTTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAAc                     <  1:167722/140‑1 (MQ=255)                                                                                                             aTTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAAc                     <  1:1257588/140‑1 (MQ=255)                                                                                                             aTTACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTACGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAAc                     <  1:62078/140‑1 (MQ=255)                                                                                                              ttACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGGCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGTCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACg                    >  2:981590/1‑140 (MQ=255)                                                                                                               tACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACgg                   <  2:1031800/140‑1 (MQ=255)                                                                                                               tACCCGCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACgg                   <  1:82649/140‑1 (MQ=255)                                                                                                                    gCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGa                       >  1:418124/1‑131 (MQ=255)                                                                                                                    gCTGCCAGCGTTGCCGCCAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGa                       <  2:418124/131‑1 (MQ=255)                                                                                                                                gccgccAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCga  <  2:681388/140‑1 (MQ=255)                                                                                                                                gccgccAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCga  >  1:681388/1‑140 (MQ=255)                                                                                                                                gccgccAGAACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCga  <  2:1134578/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TCCACGTACAGCATTTTCCAGCTCGATCGGCGAAACCGTGGAGGTTCCCAGTTTGCCGACCACCACGCCAGCCGCCGCATTGGCAAAGAAGCAGGCTTCTTCCAGCGAATTACCCGCTGCCAGCGTTGCCGCCAGGACGCCAATCACCGTGTCGCCCGCACCGGTAACGTCATACACTTCCTGCGCTTGGGTTGGCATATGCAGCGGCGCTTTACCCGGTTGCAGCAGCGACATACCCTGTTCGGAACGGGTCACTAACAGAGCCGA  >  NC_000913/3195812‑3196078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑