Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,234,267	C→T	G63G (GGG→GGA)	higB ←	ribosome‑dependent mRNA interferase toxin HigB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,234,267	0	C	T	100.0%	262.0 / NA	74	G63G (GGG→GGA)	higB	ribosome‑dependent mRNA interferase toxin HigB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/37);  total (37/37)

GCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCCCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGT  >  NC_000913/3234131‑3234402                                                                                                                                         |                                                                                                                                        gcaatcgcaatcATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCa                                                                                                                                      <  2:95443/140‑1 (MQ=255)     tcgcaatcATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACAt                                                                                                                                  >  2:916747/1‑140 (MQ=255)     tcgcaatcATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACAt                                                                                                                                  >  2:487198/1‑140 (MQ=255)      cgcaatcATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACAtt                                                                                                                                 >  2:102653/1‑140 (MQ=255)          atcATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGaaa                                                                                                                             >  1:1205095/1‑140 (MQ=255)              ttttttCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACAt                                                                                                                         <  2:1309958/140‑1 (MQ=255)              ttttttCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACAt                                                                                                                         <  1:487198/140‑1 (MQ=255)              ttttttCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACAt                                                                                                                         <  1:345207/140‑1 (MQ=255)                ttttCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATaa                                                                                                                       >  1:907634/1‑140 (MQ=255)                 tttCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAAt                                                                                                                      <  2:907634/140‑1 (MQ=255)                       cTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTAt                                                                                                                >  2:949520/1‑140 (MQ=255)                        ttAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAGCATAATGCTTATc                                                                                                               <  1:620513/140‑1 (MQ=255)                        ttAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATc                                                                                                               <  1:632945/140‑1 (MQ=255)                          aGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCa                                                                                                             <  2:118854/140‑1 (MQ=255)                          aGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATAGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCa                                                                                                             <  2:1109339/140‑1 (MQ=255)                            tACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGa                                                                                                           >  2:729202/1‑140 (MQ=255)                                 tGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATAttt                                                                                                      >  1:1147659/1‑140 (MQ=255)                                   aaCAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGa                                                                                                    >  2:337242/1‑140 (MQ=255)                                    aCAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGaa                                                                                                   >  1:335473/1‑140 (MQ=255)                                     cAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAg                                                                                                  <  1:1281356/140‑1 (MQ=255)                                     cAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAg                                                                                                  <  1:647431/140‑1 (MQ=255)                                     cAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAg                                                                                                  <  2:380838/140‑1 (MQ=255)                                      aGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGt                                                                                                 >  2:1121493/1‑140 (MQ=255)                                        cGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTa                                                                                               >  2:1123468/1‑140 (MQ=255)                                          gTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATc                                                                                             >  1:1400576/1‑140 (MQ=255)                                            aaaGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCa                                                                                           <  2:619951/140‑1 (MQ=255)                                            aaaGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCa                                                                                           <  1:1121493/140‑1 (MQ=255)                                             aaGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCag                                                                                          >  1:710013/1‑140 (MQ=255)                                             aaGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCag                                                                                          >  2:1032479/1‑140 (MQ=255)                                              aGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCaga                                                                                         <  2:1400576/140‑1 (MQ=255)                                                  aTCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAgaga                                                                                       <  1:1045442/138‑1 (MQ=255)                                                  aTCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAgaga                                                                                       >  2:1045442/1‑138 (MQ=255)                                                        ttCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGa                                                                                  <  2:674710/137‑1 (MQ=255)                                                        ttCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGa                                                                                  >  1:674710/1‑137 (MQ=255)                                                        ttCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATa                                                                               <  2:1205095/140‑1 (MQ=255)                                                        ttCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATa                                                                               <  1:102653/140‑1 (MQ=255)                                                        ttCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATa                                                                               <  1:916747/140‑1 (MQ=255)                                                           tttATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTg                                                                            <  1:949520/140‑1 (MQ=255)                                                               tGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAAt                                                                                >  2:12764/1‑132 (MQ=255)                                                               tGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAAt                                                                                <  1:12764/132‑1 (MQ=255)                                                                       aaCTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACt                                                                <  1:729202/140‑1 (MQ=255)                                                                           tCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAgg                                                            <  1:1123468/140‑1 (MQ=255)                                                                            cACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGt                                                           >  2:1088392/1‑140 (MQ=255)                                                                            cACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGt                                                           >  1:447036/1‑140 (MQ=255)                                                                                  tGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTg                                                     >  1:511315/1‑140 (MQ=255)                                                                                    tAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGaa                                                   >  1:1140498/1‑140 (MQ=255)                                                                                    tAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGaa                                                   >  2:1306967/1‑140 (MQ=255)                                                                                     aGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGaaa                                                  <  2:208707/140‑1 (MQ=255)                                                                                     aGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGaaa                                                  <  1:1306967/140‑1 (MQ=255)                                                                                     aGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGaaa                                                  <  2:511315/140‑1 (MQ=255)                                                                                      gCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAat                                                 <  2:137136/140‑1 (MQ=255)                                                                                       cACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAata                                                >  2:781295/1‑140 (MQ=255)                                                                                       cACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAata                                                <  1:1032479/140‑1 (MQ=255)                                                                                         cTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATc                                              >  1:234104/1‑140 (MQ=255)                                                                                                  ttCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCaa                                     <  2:710013/140‑1 (MQ=255)                                                                                                    cAAAAAAGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATc                                   >  2:57733/1‑140 (MQ=255)                                                                                                       aaaaGACCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCgtg                                <  2:447036/140‑1 (MQ=255)                                                                                                            aCCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCaa                                     >  2:292614/1‑130 (MQ=255)                                                                                                            aCCATTGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCaa                                     <  1:292614/130‑1 (MQ=255)                                                                                                                ttGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCaga                       <  2:234104/140‑1 (MQ=255)                                                                                                                 tGCTACAACACGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCagag                      >  1:611280/1‑140 (MQ=255)                                                                                                                       aacaCGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCaga                       <  2:556526/133‑1 (MQ=255)                                                                                                                       aacaCGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCaga                       >  1:556526/1‑133 (MQ=255)                                                                                                                        acacGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCaa               <  1:1088392/140‑1 (MQ=255)                                                                                                                           cGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGccacc                 <  2:447174/135‑1 (MQ=255)                                                                                                                           cGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGccacc                 >  1:447174/1‑135 (MQ=255)                                                                                                                           cGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTc            >  2:134120/1‑140 (MQ=255)                                                                                                                           cGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTc            >  2:743511/1‑140 (MQ=255)                                                                                                                           cGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTc            >  2:152838/1‑140 (MQ=255)                                                                                                                           cGTAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTc            >  2:998681/1‑140 (MQ=255)                                                                                                                             tAATTCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCg          >  2:1177363/1‑140 (MQ=255)                                                                                                                                ttCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCaa               <  2:1186062/132‑1 (MQ=255)                                                                                                                                ttCATTGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCaa               >  1:1186062/1‑132 (MQ=255)                                                                                                                                     tGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGt  >  1:542413/1‑140 (MQ=255)                                                                                                                                     tGCCTCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGt  >  2:325810/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GCAATCGCAATCATTTTTTCCCCTTAGTACGATGAACAGCGGTAAAGAAATCGTATTCTTTATGCGTCATAACTTCACGTATGTAGCACTTTTGCGATTCAAAAAAGACCATTGCTACAACACGTAATTCATTGCCCCCAACATTGAAAACATAATGCTTATCCAGATATTTGAAGTTATCCAGAGATGGGAATACTGCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGT  >  NC_000913/3234131‑3234402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑