Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,390,531	A→G	intergenic (+82/+52)	aaeR → / ← tldD	LysR‑type transcriptional regulator AaeR/metalloprotease TldD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,390,531	0	A	G	100.0%	240.2 / NA	68	intergenic (+82/+52)	aaeR/tldD	LysR‑type transcriptional regulator AaeR/metalloprotease TldD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/31);  total (37/31)

TGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTA  >  NC_000913/3390398‑3390664                                                                                                                                      |                                                                                                                                      tgAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTc                                                                                                                                 >  2:1395608/1‑140 (MQ=255)  gAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCg                                                                                                                                <  2:219899/140‑1 (MQ=255)  gAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCg                                                                                                                                <  1:360094/140‑1 (MQ=255)    ggTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTg                                                                                                                              <  1:644059/140‑1 (MQ=255)    ggTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTg                                                                                                                              <  2:537764/140‑1 (MQ=255)     gTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGc                                                                                                                             <  2:295953/140‑1 (MQ=255)          tAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTa                                                                                                                        >  2:47419/1‑140 (MQ=255)            aaTTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGa                                                                                                                      <  2:408630/140‑1 (MQ=255)                gTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGa                                                                                                                  >  1:27601/1‑140 (MQ=255)                      ggAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGattaatt                                                                                                            >  1:921968/1‑140 (MQ=255)                       gagaTGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGattaatta                                                                                                           <  1:292076/140‑1 (MQ=255)                       gagaTGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGattaatta                                                                                                           <  2:27601/140‑1 (MQ=255)                         gaTGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATc                                                                                                         <  2:562163/140‑1 (MQ=255)                             cACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTc                                                                                                     >  2:478392/1‑140 (MQ=255)                                gggCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGa                                                                                                  <  1:47419/140‑1 (MQ=255)                                    gcgGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGattaatta                                                                                                           <  1:1301860/127‑1 (MQ=255)                                    gcgGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGattaatta                                                                                                           >  2:1301860/1‑127 (MQ=255)                                         aaaGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGa                                                                                         <  1:1395608/140‑1 (MQ=255)                                                  tAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTAc                                                                                <  2:921968/140‑1 (MQ=255)                                                    aTTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGa                                                                                         >  2:802808/1‑129 (MQ=255)                                                    aTTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGa                                                                                         <  1:802808/129‑1 (MQ=255)                                                       cATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGt                                                                           >  2:616167/1‑140 (MQ=255)                                                       cATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGt                                                                           >  1:112630/1‑140 (MQ=255)                                                            tGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGc                                                                      >  2:867516/1‑140 (MQ=255)                                                            tGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGc                                                                      <  2:112630/140‑1 (MQ=255)                                                              tACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCa                                                                    <  2:176356/140‑1 (MQ=255)                                                               aCTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCaa                                                                   <  1:616167/140‑1 (MQ=255)                                                                    tACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTc                                                              >  1:872814/1‑140 (MQ=255)                                                                      cGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAg                                                            >  1:550139/1‑140 (MQ=255)                                                                        ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGt                                                          >  1:704119/1‑140 (MQ=255)                                                                        ttGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGt                                                          >  2:162568/1‑140 (MQ=255)                                                                           tACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTAt                                                       >  2:1054802/1‑140 (MQ=255)                                                                             cAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCg                                                     <  1:1054802/140‑1 (MQ=255)                                                                             cAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCg                                                     <  1:162568/140‑1 (MQ=255)                                                                             cAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCg                                                     >  1:241168/1‑140 (MQ=255)                                                                                 ccTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACtt                                                 >  2:30691/1‑140 (MQ=255)                                                                                  cTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACttt                                                >  1:754045/1‑140 (MQ=255)                                                                                    gtgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGa                                                    >  1:525421/1‑134 (MQ=255)                                                                                    gtgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGa                                                    <  2:525421/134‑1 (MQ=255)                                                                                     tgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTc                                                              >  2:1187939/1‑123 (MQ=255)                                                                                     tgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTc                                                              <  1:1187939/123‑1 (MQ=255)                                                                                     tgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTc                                                              <  1:478746/123‑1 (MQ=255)                                                                                     tgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTc                                                              >  2:478746/1‑123 (MQ=255)                                                                                     tgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCaa                                             >  2:1225103/1‑140 (MQ=255)                                                                                     tgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCaa                                             >  1:996517/1‑140 (MQ=255)                                                                                     tgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCaa                                             >  1:1131584/1‑140 (MQ=255)                                                                                     tgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCaa                                             >  2:331178/1‑140 (MQ=255)                                                                                     tgCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCaa                                             >  2:518951/1‑140 (MQ=255)                                                                                           cGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtgg                                       <  2:872814/140‑1 (MQ=255)                                                                                               ttCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTtggctgg                                   <  2:559121/140‑1 (MQ=255)                                                                                                 ccccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGcc                                 >  1:772899/1‑140 (MQ=255)                                                                                                 ccccTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGcc                                 <  1:867516/140‑1 (MQ=255)                                                                                                    cTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCAc                              >  1:792342/1‑140 (MQ=255)                                                                                                            aaCCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAAccc                      >  2:704114/1‑139 (MQ=255)                                                                                                            aaCCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCg                      >  2:443758/1‑140 (MQ=255)                                                                                                            aaCCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCg                      >  1:1255522/1‑140 (MQ=255)                                                                                                            aaCCCTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATAGACTTTCAACGTTGGCTGGCCCACCCCAACCg                      >  1:950885/1‑140 (MQ=255)                                                                                                              cccTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGc                    >  2:826021/1‑140 (MQ=255)                                                                                                              cccTCACCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGc                    <  2:241168/140‑1 (MQ=255)                                                                                                                   aCCCCAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACt               >  2:540192/1‑140 (MQ=255)                                                                                                                     cccAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACttt             <  2:1255522/140‑1 (MQ=255)                                                                                                                       cAAAGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGc           <  1:478392/140‑1 (MQ=255)                                                                                                                         aaGGGGCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGccc         <  1:540192/140‑1 (MQ=255)                                                                                                                             ggCGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCcttct     >  2:564013/1‑140 (MQ=255)                                                                                                                               cGAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCttt   >  1:1276181/1‑140 (MQ=255)                                                                                                                               cGAGGGGGCCGTTCGTGCACGTAGAAAGATCAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCttt   >  1:509828/1‑140 (MQ=255)                                                                                                                                gAGGGGGCCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGTCCTTCTTTa  <  2:772899/140‑1 (MQ=255)                                                                                                                                gAGGGGGCCGTTCGTGCACGTAGAAAGATCAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTa  <  2:509828/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TGAGGTCGGTAAATTGTTTCAGGAGATGCACGGGCGCGGGAAAGAGAAGTAATTCATATTGTACTGTTACGTTGTACAAACCTGTGCCAACGGGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCGTTCGTGCACGTAGAAAGATTAATTATCCTTCTGAAAATAGTGAAATTATTACGCAGTACCGCCAACAGTCAGGTTATCGACTTTCAACGTTGGCTGGCCCACGCCAACCGGCAAACTTTGCCCTTCTTTA  >  NC_000913/3390398‑3390664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑