Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,548,181	A→G	S630S (AGT→AGC)	malQ ←	4‑alpha‑glucanotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,548,181	0	A	G	100.0%	307.4 / NA	86	S630S (AGT→AGC)	malQ	4‑alpha‑glucanotransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (49/37);  total (49/37)

CATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCA  >  NC_000913/3548048‑3548317                                                                                                                                      |                                                                                                                                         catcatCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGtt                                                                                                                                     <  1:1242682/139‑1 (MQ=255) catcatCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGtt                                                                                                                                     >  2:1242682/1‑139 (MQ=255) catcatCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTa                                                                                                                                    >  2:430656/1‑140 (MQ=255)    catcGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTa                                                                                                                                    >  2:401894/1‑137 (MQ=255)    catcGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTa                                                                                                                                    <  1:401894/137‑1 (MQ=255)        ggCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCgg                                                                                                                             <  2:130761/140‑1 (MQ=255)         gCAAACATCGACTCAAGCGTTGCGGAAGGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGc                                                                                                                            >  1:87001/1‑140 (MQ=255)         gCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGc                                                                                                                            >  1:787603/1‑140 (MQ=255)             aCATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATg                                                                                                                        >  1:488122/1‑140 (MQ=255)               aTCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTa                                                                                                                      >  1:781707/1‑140 (MQ=255)                tCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAg                                                                                                                     <  1:1177329/140‑1 (MQ=255)                  gACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAgcg                                                                                                                   >  2:432802/1‑140 (MQ=255)                  gACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAgcg                                                                                                                   <  2:485207/140‑1 (MQ=255)                    cTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCt                                                                                                                 <  2:1102615/140‑1 (MQ=255)                     tCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTg                                                                                                                <  1:907807/140‑1 (MQ=255)                       aaGCGTTGCGGAAGGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCa                                                                                                              <  2:87001/140‑1 (MQ=255)                              gCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACg                                                                                                       >  1:986980/1‑140 (MQ=255)                               cGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACgg                                                                                                      >  2:910329/1‑140 (MQ=255)                                         gCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCg                                                                                            >  1:783865/1‑140 (MQ=255)                                         gCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCg                                                                                            >  1:1326150/1‑140 (MQ=255)                                                    ttttATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGAc                                                                                 <  2:986980/140‑1 (MQ=255)                                                           cTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACg                                                                          <  1:925760/140‑1 (MQ=255)                                                             ggTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACg                                                                          <  2:866561/138‑1 (MQ=255)                                                             ggTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACg                                                                          >  1:866561/1‑138 (MQ=255)                                                             ggTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGa                                                                         <  2:48383/139‑1 (MQ=255)                                                             ggTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGa                                                                         >  1:48383/1‑139 (MQ=255)                                                             ggTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGAt                                                                        >  1:684321/1‑140 (MQ=255)                                                             ggTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGAt                                                                        >  1:1370136/1‑140 (MQ=255)                                                             ggTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGAt                                                                        >  1:1329892/1‑140 (MQ=255)                                                              gTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCa                                                                                      <  2:1305778/125‑1 (MQ=255)                                                              gTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCa                                                                                      >  1:1305778/1‑125 (MQ=255)                                                              gTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATg                                                                       >  2:1237435/1‑140 (MQ=255)                                                              gTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATg                                                                       >  2:527630/1‑140 (MQ=255)                                                                 aCTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCt                                                                    >  1:405041/1‑140 (MQ=255)                                                                 aCTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCt                                                                    >  1:686564/1‑140 (MQ=255)                                                                 aCTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCt                                                                    >  1:1261950/1‑140 (MQ=255)                                                                        ccAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGccc                                                             >  2:970583/1‑140 (MQ=255)                                                                        ccAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGccc                                                             >  2:1098681/1‑140 (MQ=255)                                                                        ccAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGccc                                                             >  1:893193/1‑140 (MQ=255)                                                                        ccAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGccc                                                             >  1:1262398/1‑140 (MQ=255)                                                                        ccAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGccc                                                             >  1:1426097/1‑140 (MQ=255)                                                                          aGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCgg                                                           <  1:1237435/140‑1 (MQ=255)                                                                              atatTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACg                                                       <  2:647196/140‑1 (MQ=255)                                                                                 ttCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGttt                                                    <  1:432802/140‑1 (MQ=255)                                                                                 ttCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGttt                                                    >  2:524708/1‑140 (MQ=255)                                                                                      cGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGc                                                >  1:544356/1‑139 (MQ=255)                                                                                      cGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGc                                                <  2:544356/139‑1 (MQ=255)                                                                                      cGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCa                                               >  2:700831/1‑140 (MQ=255)                                                                                       ggTTCGGCCATATCCAGCCAGTCTTCCGGCTGGAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAg                                              >  2:966078/1‑140 (MQ=255)                                                                                         ttCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGAc                                            >  1:727075/1‑140 (MQ=255)                                                                                         ttCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGAc                                            >  1:62365/1‑140 (MQ=255)                                                                                           cGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGa                                             >  1:294437/1‑137 (MQ=255)                                                                                           cGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGa                                             <  2:294437/137‑1 (MQ=255)                                                                                            ggCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAAc                                         <  2:1426097/140‑1 (MQ=255)                                                                                            ggCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAAc                                         <  1:527630/140‑1 (MQ=255)                                                                                            ggCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAAc                                         <  2:893193/140‑1 (MQ=255)                                                                                            ggCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAAc                                         <  2:686564/140‑1 (MQ=255)                                                                                            ggCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAAc                                         <  2:1326150/140‑1 (MQ=255)                                                                                            ggCCACATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAAc                                         <  2:1262398/140‑1 (MQ=255)                                                                                              ccATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCa                                       <  2:1329892/140‑1 (MQ=255)                                                                                                atatCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCata                                     <  2:787603/140‑1 (MQ=255)                                                                                                   tCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATAttt                                  <  2:727075/140‑1 (MQ=255)                                                                                                      agccagTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATg                               <  1:530114/140‑1 (MQ=255)                                                                                                       gccagTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGc                              <  2:781707/140‑1 (MQ=255)                                                                                                        ccagTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCa                             >  1:119570/1‑140 (MQ=255)                                                                                                        ccagTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCa                             <  2:138432/140‑1 (MQ=255)                                                                                                        ccagTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCa                             >  2:1172829/1‑140 (MQ=255)                                                                                                        ccagTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGACGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCa                             >  2:143325/1‑140 (MQ=255)                                                                                                           gTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAAc                                         >  2:281178/1‑125 (MQ=255)                                                                                                           gTCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAAc                                         <  1:281178/125‑1 (MQ=255)                                                                                                            tCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGc                         >  1:941680/1‑140 (MQ=255)                                                                                                            tCTTCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGc                         >  2:1287331/1‑140 (MQ=255)                                                                                                              ttCCGGCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCAt                       >  1:600669/1‑140 (MQ=255)                                                                                                                  ggCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCcag                   <  2:405041/140‑1 (MQ=255)                                                                                                                  ggCTGTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCcag                   <  1:910329/140‑1 (MQ=255)                                                                                                                      gTAGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGTGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCagcagc               >  2:786747/1‑140 (MQ=255)                                                                                                                        aGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCa                        <  2:753481/129‑1 (MQ=255)                                                                                                                        aGTCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCa                        >  1:753481/1‑129 (MQ=255)                                                                                                                          tCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCtt           >  1:878919/1‑140 (MQ=255)                                                                                                                          tCCTAACAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTACATCCAGCAGCCCtt           >  2:558348/1‑140 (MQ=255)                                                                                                                               aCAGAGCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTc      >  2:835092/1‑140 (MQ=255)                                                                                                                                 agagCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGc    >  2:1063186/1‑140 (MQ=255)                                                                                                                                 agagCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGc    <  1:524708/140‑1 (MQ=255)                                                                                                                                 agagCGCTGTCACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGc    <  2:878919/140‑1 (MQ=255)                                                                                                                                  gagCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGcc   >  1:662259/1‑140 (MQ=255)                                                                                                                                   agCGCTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCa  <  1:966078/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         CATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGCCAGGAATATTCACCGGTTCGGCCATATCCAGCCAGTCTTCCGGCTGTAGTCCTAACAGAGCACTGTTACTGTCGGCAATGTAGCGCTGCAAACCACGGTTCAGCGTCGGCGTCATCGACATCAACGATGCCTTATGCCCGGCACGTTTCGGCAGACAACCATATTTATGCAGTGCATCCAGCAGCCCTTGCTTCGCCA  >  NC_000913/3548048‑3548317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑