Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	338,266	A→G	S497G (AGC→GGC)	yahF →	putative acyl‑CoA synthetase YahF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	338,266	0	A	G	100.0%	264.1 / NA	73	S497G (AGC→GGC)	yahF	putative acyl‑CoA synthetase YahF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/29);  total (44/29)

GCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGCCATGTTTAGCGATGACCTGAAAAAG  >  NC_000913/338134‑338402                                                                                                                                     |                                                                                                                                         gcgaaagcgaTCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCAc                                                                                                                                   <  1:25117/140‑1 (MQ=255)  cgaaagcgaTCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCTTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGACGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCAcc                                                                                                                                  >  2:595000/1‑140 (MQ=255)                       aaGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGt                                                                                                             <  1:516331/140‑1 (MQ=255)                              cgAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTa                                                                                                      >  2:50238/1‑140 (MQ=255)                              cgAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTa                                                                                                      >  1:249963/1‑140 (MQ=255)                               gAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTAt                                                                                                     <  1:952193/140‑1 (MQ=255)                                 gTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCt                                                                                                   <  1:670120/140‑1 (MQ=255)                                    gATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCa                                                                                                <  1:1033210/140‑1 (MQ=255)                                    gATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCa                                                                                                <  2:249963/140‑1 (MQ=255)                                    gATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCa                                                                                                <  1:1219007/140‑1 (MQ=255)                                          tctcGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAgggg                                                                                          <  1:895919/140‑1 (MQ=255)                                           ctcGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGgga                                                                                         >  1:888781/1‑140 (MQ=255)                                               ccTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGc                                                                                                 <  2:475900/128‑1 (MQ=255)                                               ccTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGc                                                                                                 >  1:475900/1‑128 (MQ=255)                                               ccTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGaa                                                                                     >  2:1185082/1‑140 (MQ=255)                                               ccTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGaa                                                                                     >  1:408797/1‑140 (MQ=255)                                                cTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAg                                                                                    <  1:1185082/140‑1 (MQ=255)                                                   tgtgCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCt                                                                                 >  2:838984/1‑140 (MQ=255)                                                   tgtgCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCt                                                                                 >  1:343020/1‑140 (MQ=255)                                                   tgtgCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCt                                                                                 >  2:259304/1‑140 (MQ=255)                                                      gCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCtgat                                                                              <  1:50238/140‑1 (MQ=255)                                                        tGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCtgatga                                                                            >  1:1424/1‑140 (MQ=255)                                                          ggTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGc                                                                          >  2:259273/1‑140 (MQ=255)                                                             aCCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAg                                                                       >  2:269412/1‑140 (MQ=255)                                                              ccGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGACGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGt                                                                      <  1:595000/140‑1 (MQ=255)                                                               cGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTc                                                                     >  1:388918/1‑140 (MQ=255)                                                               cGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTc                                                                     >  1:959286/1‑140 (MQ=255)                                                               cGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTc                                                                     >  1:231492/1‑140 (MQ=255)                                                                 aTCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCtgatga                                                                            <  2:632382/131‑1 (MQ=255)                                                                 aTCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCtgatga                                                                            >  1:632382/1‑131 (MQ=255)                                                                   cTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTg                                                                 <  1:259304/140‑1 (MQ=255)                                                                    ttGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGt                                                                >  1:355949/1‑140 (MQ=255)                                                                      gATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACt                                                                  >  2:342527/1‑136 (MQ=255)                                                                      gATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACt                                                                  <  1:342527/136‑1 (MQ=255)                                                                      gATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGttt                                                              >  2:1055978/1‑140 (MQ=255)                                                                          cGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGcc                                                          <  1:1055978/140‑1 (MQ=255)                                                                            ccATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCaa                                                        <  2:1289879/140‑1 (MQ=255)                                                                             cATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGt                                                                      <  2:1350296/125‑1 (MQ=255)                                                                             cATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGt                                                                      >  1:1350296/1‑125 (MQ=255)                                                                             cATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAAc                                                       >  1:843965/1‑140 (MQ=255)                                                                                cGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCAt                                                    >  2:520467/1‑140 (MQ=255)                                                                                cGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCAt                                                    >  1:530964/1‑140 (MQ=255)                                                                                cGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCAt                                                    >  2:573644/1‑140 (MQ=255)                                                                                     gAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAAc                                               >  1:1176134/1‑140 (MQ=255)                                                                                      aacaacAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACg                                              >  2:526022/1‑140 (MQ=255)                                                                                          acaaAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGttat                                          >  1:211854/1‑140 (MQ=255)                                                                                          acaaAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGttat                                          >  2:59912/1‑140 (MQ=255)                                                                                          acaaAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGttat                                          >  2:1368352/1‑140 (MQ=255)                                                                                           caaAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGttatt                                         >  1:457265/1‑140 (MQ=255)                                                                                                ccAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGt                                    >  1:17136/1‑140 (MQ=255)                                                                                                ccAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGt                                    >  1:523960/1‑140 (MQ=255)                                                                                                 cAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTc                                   >  1:956548/1‑140 (MQ=255)                                                                                                   gATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCgg                                 >  1:914269/1‑140 (MQ=255)                                                                                                     tgctgcTTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCa                               >  2:1293792/1‑140 (MQ=255)                                                                                                        tgctTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCg                            <  2:523960/140‑1 (MQ=255)                                                                                                         gctTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGc                           <  2:888781/140‑1 (MQ=255)                                                                                                          ctTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGcc                          >  2:519132/1‑140 (MQ=255)                                                                                                          ctTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGcc                          <  1:1293792/140‑1 (MQ=255)                                                                                                          ctTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGcc                          <  2:355949/140‑1 (MQ=255)                                                                                                          ctTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGcc                          >  1:1188237/1‑140 (MQ=255)                                                                                                          ctTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGcc                          <  2:956548/140‑1 (MQ=255)                                                                                                          ctTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGcc                          >  1:1298853/1‑140 (MQ=255)                                                                                                          ctTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGcc                          <  2:388918/140‑1 (MQ=255)                                                                                                          ctTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGcc                          <  1:520467/140‑1 (MQ=255)                                                                                                          ctTGATGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGcc                          <  1:573644/140‑1 (MQ=255)                                                                                                              aTGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCgg                                 >  2:102750/1‑129 (MQ=255)                                                                                                              aTGCCGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCgg                                 <  1:102750/129‑1 (MQ=255)                                                                                                                  cGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGcc                          <  2:502435/132‑1 (MQ=255)                                                                                                                  cGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGcc                          >  1:502435/1‑132 (MQ=255)                                                                                                                  cGGAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGCCATGTTTAg                  >  1:38020/1‑140 (MQ=255)                                                                                                                    gAGTGATTCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGCCATGTTTAGCg                <  2:211854/140‑1 (MQ=255)                                                                                                                           tCTGGCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGCCATGTTTAGCGATGACCt         >  1:1088241/1‑140 (MQ=255)                                                                                                                               gCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGCCATGTTTAGCGATGACCTGaaa     >  2:405418/1‑140 (MQ=255)                                                                                                                               gCGAGCGGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGCCATGTTTAGCGATGACCTGaaa     >  1:813307/1‑140 (MQ=255)                                                                                                                                  agcggcagcACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGCCATGTTTAGCGATGACCTGAAAAag  <  2:457265/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GCGAAAGCGATCGCCGCTGCCGAAGGACGCGAGTTGATCATTCTCGCCTATGTGCTGGGTACCGATCTTGATACGCCATCGTTAGAACAACAAAGCCAGATGCTGCTTGATGCCGGAGTGATTCTGGCGAGCAGCAGCACCAATACCGGATTGCTGGCGCGTGAATTTATCTGCAAAGGGGAGGAAGCCTGATGAGCCAGTCACTGTTTAGCCAACCATTGAACGTTATTAACGTCGGCATCGCCATGTTTAGCGATGACCTGAAAAAG  >  NC_000913/338134‑338402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑