Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,568	G→A	Q85* (CAA→TAA)	ilvY ←	DNA‑binding transcriptional dual regulator IlvY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,568	0	G	A	100.0%	242.5 / NA	69	Q85* (CAA→TAA)	ilvY	DNA‑binding transcriptional dual regulator IlvY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/32);  total (37/32)

TGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCGGCTGACCGAGATCTTCTTCC  >  NC_000913/3957431‑3957704                                                                                                                                          |                                                                                                                                         tGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACt                                                                                                                                        <  1:1283694/140‑1 (MQ=255)    cGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGAt                                                                                                                                     <  2:346528/140‑1 (MQ=255)    cGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGAt                                                                                                                                     <  2:332071/140‑1 (MQ=255)        tCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGAt                                                                                                                                 <  2:116666/140‑1 (MQ=255)            cAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGgtg                                                                                                                             <  1:1329397/140‑1 (MQ=255)             agtagtAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGgtgt                                                                                                                            >  1:1076288/1‑140 (MQ=255)              gtagtaAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGtgtg                                                                                                                           >  2:439703/1‑140 (MQ=255)                 gtaAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGgcg                                                                                                                        <  2:460347/140‑1 (MQ=255)                  taAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGgcgc                                                                                                                       >  2:684952/1‑140 (MQ=255)                   aaGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCa                                                                                                                      <  1:1337140/140‑1 (MQ=255)                   aaGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCa                                                                                                                      <  1:12046/140‑1 (MQ=255)                   aaGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCa                                                                                                                      <  2:276639/140‑1 (MQ=255)                    aGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCaa                                                                                                                     <  2:1076288/140‑1 (MQ=255)                       ttAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGgtg                                                                                                                             >  2:1378945/1‑129 (MQ=255)                       ttAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGgtg                                                                                                                             <  1:1378945/129‑1 (MQ=255)                         aaTCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAActgct                                                                                                                >  2:974563/1‑140 (MQ=255)                         aaTCTCCACCGACGGGAGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAActgct                                                                                                                >  1:1358871/1‑140 (MQ=255)                          aTCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAActgctg                                                                                                               >  1:1051082/1‑140 (MQ=255)                           tctcCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCGGa                                                                                                              <  2:1051082/140‑1 (MQ=255)                             tcCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATa                                                                                                            <  2:516000/140‑1 (MQ=255)                               cACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACt                                                                                                          >  2:994506/1‑140 (MQ=255)                                aCCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTg                                                                                                         >  2:824560/1‑140 (MQ=255)                                  cGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCa                                                                                                       <  1:684952/140‑1 (MQ=255)                                           ttCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGttt                                                                                              >  1:347839/1‑140 (MQ=255)                                           ttCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGttt                                                                                              >  1:1043496/1‑140 (MQ=255)                                                cgcgGAAGCGATCCGGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTgg                                                                                         >  2:527993/1‑140 (MQ=255)                                                cgcgGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCa                                                                                                       <  2:693160/126‑1 (MQ=255)                                                cgcgGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCa                                                                                                       >  1:693160/1‑126 (MQ=255)                                                cgcgGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTgg                                                                                         >  1:562376/1‑140 (MQ=255)                                                cgcgGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTgg                                                                                         >  1:1263989/1‑140 (MQ=255)                                                  cgGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGc                                                                                       >  2:72971/1‑140 (MQ=255)                                                     aaGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGaa                                                                                    >  1:523364/1‑140 (MQ=255)                                                                  tCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGctct                                                                       >  1:692687/1‑140 (MQ=255)                                                                   cggcggCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACgcgc                                                                             <  2:177512/133‑1 (MQ=255)                                                                   cggcggCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACgcgc                                                                             >  1:177512/1‑133 (MQ=255)                                                                     gcggcAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCg                                                                    >  1:54548/1‑140 (MQ=255)                                                                     gcggcAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCg                                                                    >  2:616768/1‑140 (MQ=255)                                                                     gcggcAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCg                                                                    >  2:202900/1‑140 (MQ=255)                                                                     gcggcAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGGGGCGCAACTGCTGATACTGCAACAGCGTTTGCAGGTCGAAAACGCGCAGCTCTTCg                                                                    >  1:342656/1‑140 (MQ=255)                                                                      cggcAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGc                                                                   >  1:287243/1‑140 (MQ=255)                                                                      cggcAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGc                                                                   <  2:523364/140‑1 (MQ=255)                                                                       ggcAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGcc                                                                  <  2:347839/140‑1 (MQ=255)                                                                              ggCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTtca                                                           <  1:1077026/140‑1 (MQ=255)                                                                                   tAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTtcagtcag                                                      <  2:1358871/140‑1 (MQ=255)                                                                                     ggCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATAATGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCg                                                    <  2:1384675/140‑1 (MQ=255)                                                                                        aGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTtca                                                           >  2:1118860/1‑130 (MQ=255)                                                                                        aGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTtca                                                           <  1:1118860/130‑1 (MQ=255)                                                                                          cGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCAcc                                               <  1:824560/140‑1 (MQ=255)                                                                                             tCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCACTTCAGTCAGCGTCACCGTg                                            <  1:616768/140‑1 (MQ=255)                                                                                               aCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCg                                          >  2:325818/1‑140 (MQ=255)                                                                                                    gCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTAt                                     <  2:54548/140‑1 (MQ=255)                                                                                                      agaagaTATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATcg                                   <  1:360563/140‑1 (MQ=255)                                                                                                        aagaTATGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATcgcg                                 <  2:287243/140‑1 (MQ=255)                                                                                                           atatGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGcac                              >  2:35128/1‑140 (MQ=255)                                                                                                             atGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACaa                            >  1:566112/1‑140 (MQ=255)                                                                                                              tGTAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACaaa                           <  1:35128/140‑1 (MQ=255)                                                                                                                tAATTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACa                         >  2:1117484/1‑140 (MQ=255)                                                                                                                  aTTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGc                       >  1:1101065/1‑140 (MQ=255)                                                                                                                  aTTCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGc                       >  1:1073527/1‑140 (MQ=255)                                                                                                                   ttCGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCg                      <  1:994506/140‑1 (MQ=255)                                                                                                                    tCGCCAGAGAGCGACGGCACTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCgg                     <  2:1043496/140‑1 (MQ=255)                                                                                                                     cGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCGGc                    >  2:1425034/1‑140 (MQ=255)                                                                                                                     cGCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCGGc                    <  1:597025/140‑1 (MQ=255)                                                                                                                      gCCAGAGAGCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCGGCt                   >  2:224641/1‑140 (MQ=255)                                                                                                                          gagagCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCGGCTGAcc               >  2:137277/1‑140 (MQ=255)                                                                                                                           agagCGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCGGCTGACCg              >  1:1264149/1‑140 (MQ=255)                                                                                                                               cGACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCGGCTGACCGAGAt          >  1:1276958/1‑140 (MQ=255)                                                                                                                                gACGGCCCTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCGGCTGACCGAGAtc         >  1:118615/1‑140 (MQ=255)                                                                                                                                     cccTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCGGCTGACCGAGATcttctt    >  2:611553/1‑140 (MQ=255)                                                                                                                                       cTTACTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCGGCTGACCGAGATCTTCTTcc  <  2:1263989/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCACCGTGCGGTTATCGCGCACAAACAGCGGCTGACCGAGATCTTCTTCC  >  NC_000913/3957431‑3957704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑