Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,995,913	C→T	R111C (CGC→TGC)	yigA →	conserved protein YigA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,995,913	0	C	T	100.0%	343.0 / NA	95	R111C (CGC→TGC)	yigA	conserved protein YigA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (61/34);  total (61/34)

TCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTT  >  NC_000913/3995777‑3996049                                                                                                                                         |                                                                                                                                         tCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCt                                                                                                                                       >  2:1348256/1‑140 (MQ=255) tCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCt                                                                                                                                       >  1:844555/1‑140 (MQ=255) tCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCt                                                                                                                                       >  2:1157792/1‑140 (MQ=255)  cATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCActgctg                                                                                                                                      <  2:288655/140‑1 (MQ=255)    tGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTggg                                                                                                                                    >  1:253603/1‑140 (MQ=255)     gTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGgc                                                                                                                                   >  2:673445/1‑140 (MQ=255)     gTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGgc                                                                                                                                   >  2:384090/1‑140 (MQ=255)     gTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGgc                                                                                                                                   >  2:860654/1‑140 (MQ=255)        cTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGgcgcg                                                                                                                                <  2:502453/140‑1 (MQ=255)        cTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGgcgcg                                                                                                                                >  2:735492/1‑140 (MQ=255)         tGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGgcgcgc                                                                                                                               >  2:288262/1‑140 (MQ=255)           gAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGa                                                                                                                             >  1:180082/1‑140 (MQ=255)           gAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGa                                                                                                                             >  1:1277463/1‑140 (MQ=255)            aaGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGAt                                                                                                                            <  1:836066/140‑1 (MQ=255)              gagaACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGAtct                                                                                                                          <  2:1072250/140‑1 (MQ=255)               agaACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGAtctc                                                                                                                         >  1:791105/1‑140 (MQ=255)                gaACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCg                                                                                                                        >  1:841748/1‑140 (MQ=255)                 aaCATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCgg                                                                                                                       >  2:485302/1‑140 (MQ=255)                    aTGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTggg                                                                                                                                    <  2:1173018/124‑1 (MQ=255)                    aTGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTggg                                                                                                                                    >  1:1173018/1‑124 (MQ=255)                     tGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTg                                                                                                                   >  2:986015/1‑140 (MQ=255)                      ggCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTgg                                                                                                                  >  2:241906/1‑140 (MQ=255)                       gcgcTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGc                                                                                                                 >  1:1053148/1‑140 (MQ=255)                        cgcTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCa                                                                                                                >  2:1203050/1‑140 (MQ=255)                          cTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAgg                                                                                                              <  2:276740/140‑1 (MQ=255)                            gTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTg                                                                                                            <  2:274395/140‑1 (MQ=255)                            gTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGACTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTg                                                                                                            <  2:1334640/140‑1 (MQ=255)                                aTGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAg                                                                                                        >  2:494563/1‑140 (MQ=255)                                    aaCAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTg                                                                                                    <  1:735492/140‑1 (MQ=255)                                       aGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGa                                                                                                         <  2:338712/132‑1 (MQ=255)                                       aGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGa                                                                                                         >  1:338712/1‑132 (MQ=255)                                       aGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTgcgc                                                                                                 <  2:1351213/140‑1 (MQ=255)                                       aGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTgcgc                                                                                                 >  2:920275/1‑140 (MQ=255)                                       aGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTgcgc                                                                                                 >  1:1294479/1‑140 (MQ=255)                                        ggCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGcc                                                                                                <  1:986015/140‑1 (MQ=255)                                              cGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTc                                                                                          >  1:27468/1‑140 (MQ=255)                                              cGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTc                                                                                          >  2:8055/1‑140 (MQ=255)                                                  aaCGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGa                                                                                      >  2:336548/1‑140 (MQ=255)                                                   aCGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGAt                                                                                     >  1:746794/1‑140 (MQ=255)                                                   aCGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGAt                                                                                     >  2:1060195/1‑140 (MQ=255)                                                   aCGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGAt                                                                                     <  1:797614/140‑1 (MQ=255)                                                   aCGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGAt                                                                                     >  1:799907/1‑140 (MQ=255)                                                    cGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATc                                                                                    >  1:860369/1‑140 (MQ=255)                                                       aGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCt                                                                                 >  1:404652/1‑140 (MQ=255)                                                        ggCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTg                                                                                <  1:920275/140‑1 (MQ=255)                                                              ttttATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCtt                                                                          >  2:990305/1‑140 (MQ=255)                                                                   tCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTg                                                                     <  1:83745/140‑1 (MQ=255)                                                                   tCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTg                                                                     >  2:83745/1‑140 (MQ=255)                                                                        tACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCg                                                                >  2:714633/1‑140 (MQ=255)                                                                        tACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCCGATCGCTGGCGCTTAGGTGCGCCg                                                                >  2:267566/1‑140 (MQ=255)                                                                           tctACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCg                                                             <  1:1348256/140‑1 (MQ=255)                                                                              aCCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAAc                                                          >  2:477068/1‑140 (MQ=255)                                                                              aCCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAAc                                                          <  2:404652/140‑1 (MQ=255)                                                                               ccTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAAcc                                                         >  2:1071385/1‑140 (MQ=255)                                                                                cTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACca                                                        <  1:288262/140‑1 (MQ=255)                                                                                   cAGCGCAGTCTCACCGCCGCCAGCAGTTTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCacac                                                     >  1:1235618/1‑140 (MQ=255)                                                                                   cAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCacac                                                     <  1:714633/140‑1 (MQ=255)                                                                                    aGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACt                                                    >  2:151352/1‑140 (MQ=255)                                                                                    aGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACt                                                    >  2:248632/1‑140 (MQ=255)                                                                                    aGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACt                                                    >  2:533597/1‑140 (MQ=255)                                                                                     gcgcAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGaa                                                           >  2:1283523/1‑132 (MQ=255)                                                                                     gcgcAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGaa                                                           >  2:1219457/1‑132 (MQ=255)                                                                                     gcgcAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGaa                                                           <  1:1219457/132‑1 (MQ=255)                                                                                     gcgcAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGaa                                                           <  1:1283523/132‑1 (MQ=255)                                                                                     gcgcAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTc                                                   >  2:1291839/1‑140 (MQ=255)                                                                                     gcgcAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTc                                                   <  1:241906/140‑1 (MQ=255)                                                                                     gcgcAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTc                                                   >  1:550047/1‑140 (MQ=255)                                                                                       gcAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCAt                                                 >  2:10919/1‑140 (MQ=255)                                                                                        cAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATc                                                <  1:10919/140‑1 (MQ=255)                                                                                         aGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCt                                               >  2:406307/1‑140 (MQ=255)                                                                                         aGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCt                                               >  1:748728/1‑140 (MQ=255)                                                                                           tctcACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTgg                                             >  2:1088801/1‑140 (MQ=255)                                                                                                ccgccgCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTa                                        <  2:1235618/140‑1 (MQ=255)                                                                                                     gccAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCAt                                                 >  2:894483/1‑126 (MQ=255)                                                                                                     gccAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCAt                                                 <  1:894483/126‑1 (MQ=255)                                                                                                     gccAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCg                                   >  1:57526/1‑140 (MQ=255)                                                                                                     gccAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCg                                   >  1:579914/1‑140 (MQ=255)                                                                                                     gccAGCAGTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGGTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCg                                   >  2:1250930/1‑140 (MQ=255)                                                                                                        agcagTCTCGACGATATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCa                                <  1:1071385/140‑1 (MQ=255)                                                                                                                  acgaTATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGaa                      <  2:799907/140‑1 (MQ=255)                                                                                                                  acgaTATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGaa                      <  2:1394084/140‑1 (MQ=255)                                                                                                                  acgaTATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGaa                      <  1:384090/140‑1 (MQ=255)                                                                                                                  acgaTATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCCGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGaa                      <  1:267566/140‑1 (MQ=255)                                                                                                                    gaTATGCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAAcc                    <  2:844555/140‑1 (MQ=255)                                                                                                                         gCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGc               >  1:456804/1‑140 (MQ=255)                                                                                                                         gCTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGc               <  2:748728/140‑1 (MQ=255)                                                                                                                          cTGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCg              <  2:783194/140‑1 (MQ=255)                                                                                                                           tGATGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGt             >  2:1377047/1‑140 (MQ=255)                                                                                                                             aTGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTAt           >  1:762159/1‑140 (MQ=255)                                                                                                                             aTGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTAt           >  2:1025562/1‑140 (MQ=255)                                                                                                                             aTGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTAt           >  1:1131011/1‑140 (MQ=255)                                                                                                                             aTGCGCTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTAt           >  1:964633/1‑140 (MQ=255)                                                                                                                                  cTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCg                        <  1:55188/122‑1 (MQ=255)                                                                                                                                  cTTTCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCg                        >  2:55188/1‑122 (MQ=255)                                                                                                                                     tCACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGtt   >  1:151262/1‑140 (MQ=255)                                                                                                                                      cACTGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGttt  <  2:456804/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TCATGTTCTGGAAGAGAACATGGCGCTGTTGATGGAACAGGCTATCGCCAACGAAGGCCTGTTTTATCGCCTACTCTACCTGCAGCGCAGTCTCACCGCCGCCAGCAGTCTCGACGATATGCTGATGCGCTTTCACCGCTGGGCGCGCGATCTCGGCCTGGCAGGTGCGAGTCTGCGCCTGTTTCCGGATCGCTGGCGCTTAGGTGCGCCGTCGAACCACACTCATCTGGCATTAAGCCGTCAGTCTTTCGAACCGCTGCGTATTCAGCGTTT  >  NC_000913/3995777‑3996049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑