Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,077,790	T→C	R114R (CGT→CGC)	yiiD →	putative acetyltransferase YiiD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,077,790	0	T	C	100.0%	310.9 / NA	88	R114R (CGT→CGC)	yiiD	putative acetyltransferase YiiD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/37);  total (51/37)

TAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTTGATGATTAAG  >  NC_000913/4077655‑4077925                                                                                                                                        |                                                                                                                                        tAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAgg                                                                                                                                     <  2:13301/140‑1 (MQ=255) tAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAgg                                                                                                                                     <  2:1232286/140‑1 (MQ=255)    tCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCGGGAAg                                                                                                                                  <  1:364279/140‑1 (MQ=255)    tCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAg                                                                                                                                  <  1:936570/140‑1 (MQ=255)         tggCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGtt                                                                                                                             >  1:713427/1‑140 (MQ=255)         tggCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGtt                                                                                                                             >  1:645342/1‑140 (MQ=255)         tggCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGtt                                                                                                                             >  1:1336872/1‑140 (MQ=255)           gCGGTAGGCCGACTGTATATTAATGCCGACAATGAAACGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTaa                                                                                                                           >  1:721201/1‑140 (MQ=255)               tAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAgcgc                                                                                                                       <  2:954436/140‑1 (MQ=255)                 ggCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGt                                                                                                                     <  2:1336872/140‑1 (MQ=255)                   ccGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGa                                                                                                                   <  2:868106/140‑1 (MQ=255)                          tataTTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAg                                                                                                            <  2:1005270/140‑1 (MQ=255)                            tatTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAgcg                                                                                                          <  2:1095041/140‑1 (MQ=255)                                 aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGt                                                                                                     >  2:1216402/1‑140 (MQ=255)                                 aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGt                                                                                                     >  2:830011/1‑140 (MQ=255)                                 aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGt                                                                                                     >  1:214709/1‑140 (MQ=255)                                 aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGt                                                                                                     >  2:799120/1‑140 (MQ=255)                                 aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGt                                                                                                     >  1:26960/1‑140 (MQ=255)                                 aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGt                                                                                                     >  2:431667/1‑140 (MQ=255)                                 aTGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGt                                                                                                     >  1:1205583/1‑140 (MQ=255)                                  tGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTg                                                                                                    >  2:235571/1‑140 (MQ=255)                                  tGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTg                                                                                                    >  1:524938/1‑140 (MQ=255)                                  tGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGATAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTg                                                                                                    >  2:1275499/1‑140 (MQ=255)                                    ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAg                                                                                                            >  2:762743/1‑130 (MQ=255)                                    ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAg                                                                                                            <  1:762743/130‑1 (MQ=255)                                    ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                                                                  >  2:157558/1‑140 (MQ=255)                                    ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                                                                  >  2:543831/1‑140 (MQ=255)                                    ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                                                                  >  2:572574/1‑140 (MQ=255)                                    ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                                                                  >  2:573736/1‑140 (MQ=255)                                    ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                                                                  >  1:221038/1‑140 (MQ=255)                                    ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                                                                  >  1:607991/1‑140 (MQ=255)                                    ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGaa                                                                                                  >  1:375365/1‑140 (MQ=255)                                    ccGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGAGACCTGTAGCGCCCGTTaa                                                                                                  >  2:291412/1‑140 (MQ=255)                                       aCAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTa                                                                                                             <  2:1113626/126‑1 (MQ=255)                                       aCAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTa                                                                                                             >  1:1113626/1‑126 (MQ=255)                                              aGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTgg                                                                                        >  2:805582/1‑140 (MQ=255)                                                  tCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGtt                                                                                    >  1:1106705/1‑140 (MQ=255)                                                    cATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGtttt                                                                                  >  1:92068/1‑140 (MQ=255)                                                     aTTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGttttt                                                                                 >  2:933805/1‑140 (MQ=255)                                                     aTTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGttttt                                                                                 >  2:881291/1‑140 (MQ=255)                                                         gCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGcc                                                                             <  2:607991/140‑1 (MQ=255)                                                         gCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGcc                                                                             <  1:881291/140‑1 (MQ=255)                                                                 gCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTggg                                                                     <  2:1205583/140‑1 (MQ=255)                                                                 gCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTggg                                                                     <  1:431667/140‑1 (MQ=255)                                                                 gCCGTTCAACCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTggg                                                                     <  1:716795/140‑1 (MQ=255)                                                                     ttCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCg                                                                               >  2:420317/1‑126 (MQ=255)                                                                     ttCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCg                                                                               <  1:420317/126‑1 (MQ=255)                                                                     ttCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTggg                                                                     <  1:1111462/136‑1 (MQ=255)                                                                     ttCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTggg                                                                     >  2:1111462/1‑136 (MQ=255)                                                                     ttCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGttt                                                                 >  2:669190/1‑140 (MQ=255)                                                                     ttCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGttt                                                                 >  2:549063/1‑140 (MQ=255)                                                                          cccGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTaa                                                            <  2:375365/140‑1 (MQ=255)                                                                               cGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAgg                                                       <  1:805582/140‑1 (MQ=255)                                                                               cGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAgg                                                       <  1:549063/140‑1 (MQ=255)                                                                                 tGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGga                                                     <  2:92068/140‑1 (MQ=255)                                                                                        cAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATca                                              <  2:713427/140‑1 (MQ=255)                                                                                          aaGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATcacc                                            <  1:1275499/140‑1 (MQ=255)                                                                                           aGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATcacca                                           >  2:1119691/1‑140 (MQ=255)                                                                                           aGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATcacca                                           >  2:154955/1‑140 (MQ=255)                                                                                           aGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGG‑TTGTTTATTAAGGAGAAATcaccac                                          >  1:423437/1‑140 (MQ=255)                                                                                           aGGGTTAGGCACGCTGATGGCGATGACCCTGGAGCCGGCGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATcacca                                           >  1:105473/1‑140 (MQ=255)                                                                                               ttAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATTACCACGcc                                       <  1:1216402/140‑1 (MQ=255)                                                                                               ttAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGcc                                       <  1:874732/140‑1 (MQ=255)                                                                                                tAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCa                                      <  2:221038/140‑1 (MQ=255)                                                                                                tAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCa                                      >  1:903330/1‑140 (MQ=255)                                                                                                tAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCa                                      >  1:809845/1‑140 (MQ=255)                                                                                                  ggCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAac                                    >  1:732659/1‑140 (MQ=255)                                                                                                       gCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACg                                         <  1:302751/130‑1 (MQ=255)                                                                                                       gCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACg                                         >  2:302751/1‑130 (MQ=255)                                                                                                       gCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAAccacca                               >  1:650912/1‑140 (MQ=255)                                                                                                       gCTGATGGCGATGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAAccacca                               >  2:921072/1‑140 (MQ=255)                                                                                                                  tGACCCTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGc                    <  1:291412/140‑1 (MQ=255)                                                                                                                     cccTGGAGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCAt                 <  1:799120/140‑1 (MQ=255)                                                                                                                           ggTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTtg           <  2:809845/139‑1 (MQ=255)                                                                                                                           aGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCGCCACGCCGATTCGCCATTTTTtg           <  2:214709/140‑1 (MQ=255)                                                                                                                           aGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTtg           <  1:295964/140‑1 (MQ=255)                                                                                                                           aGTCGGTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTtg           <  2:732659/140‑1 (MQ=255)                                                                                                                               ggTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCAt                 <  1:293999/130‑1 (MQ=255)                                                                                                                               ggTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCAt                 >  2:293999/1‑130 (MQ=255)                                                                                                                               ggTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTtgatga       >  1:318412/1‑140 (MQ=255)                                                                                                                               ggTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTtgatga       >  1:493227/1‑140 (MQ=255)                                                                                                                                gTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTtg           >  1:704223/1‑135 (MQ=255)                                                                                                                                gTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTtg           <  2:704223/135‑1 (MQ=255)                                                                                                                                gTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTTgatgat      >  2:1299218/1‑140 (MQ=255)                                                                                                                                gTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTTgatgat      >  1:28337/1‑140 (MQ=255)                                                                                                                                gTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTTgatgat      >  1:697013/1‑140 (MQ=255)                                                                                                                                gTGGCGCGCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCGGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTTgatgat      >  1:150795/1‑140 (MQ=255)                                                                                                                                    cgcgCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTTGATGATTAAg  <  2:721201/140‑1 (MQ=255)                                                                                                                                    cgcgCCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTTGATGATTAAg  >  1:759844/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TAATCTGGTGGCGGTAGGCCGACTGTATATTAATGCCGACAATGAAGCGTCCATTCGCTTTATGGCCGTTCATCCCGACGTGCAGGACAAAGGGTTAGGCACGCTGATGGCGATGACCCTGGAGTCGGTGGCGCGTCAGGAAGGCGTTAAGCGCGTGACCTGTAGCGCCCGTGAAGACGCGGTGGAGTTTTTCGCCAAGCTGGGGTTTGTTAATCAGGGAGAAATCACCACGCCAACCACCACGCCGATTCGCCATTTTTTGATGATTAAG  >  NC_000913/4077655‑4077925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑