Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,108,469	T→C	R306R (CGT→CGC)	pfkA →	6‑phosphofructokinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,108,469	0	T	C	100.0%	207.7 / NA	58	R306R (CGT→CGC)	pfkA	6‑phosphofructokinase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/33);  total (25/33)

GGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGTCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATCAGG  >  NC_000913/4108343‑4108594                                                                                                                               |                                                                                                                              ggCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTg                                                                                                                  <  2:85237/140‑1 (MQ=255) ggCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTg                                                                                                                  <  1:899372/140‑1 (MQ=255) ggCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTg                                                                                                                  <  1:802092/140‑1 (MQ=255) ggCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTg                                                                                                                  <  2:264720/140‑1 (MQ=255)   cTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGAc                                                                                                                <  1:1202031/140‑1 (MQ=255)       ccGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGGATCCAGAACGAACAGCTGGGTCACCACGACATCATCCACGCTATCGAAAAAATGAAGCGCCCGTTCAAAAGGGActggc                                                                                                            >  2:645639/1‑140 (MQ=255)            tGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGAc                                                                                                       <  1:716875/140‑1 (MQ=255)                cgcTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTgcg                                                                                                   <  1:1130727/140‑1 (MQ=255)                      acagaTCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGaaaa                                                                                             <  1:829817/136‑1 (MQ=255)                        cTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGaaagaa                                                                                           <  1:645639/140‑1 (MQ=255)                                 tgctgctgGCAGGTTACGGCGGTCGTTGTGTAGGGATCCCGAAAGAAAAGCTGGGTCACCACGACATCATCGACGCTTTCGAAAAAATGAAGCGCCCGTTCAAAAGGGACTGGCTGGACTGCGCGGAAAAAATGTATTaa                                                                                  >  2:585704/1‑140 (MQ=255)                                   ctgctgGCAGGTTACGGCGGTCGTTGTGTAGGGATCCAGAACGAACAGCTGGGTCACCCCGACATCATCGACGCTATCGAAAAAATGAAGCGCCCGTTCAAAGGGGAATGGCTGGACTGCGCGAAAAAAATGTATTAATg                                                                                >  2:1167445/1‑140 (MQ=255)                                   ctgctgGCAGGTTACGGCGGTCGTTGTGTAGGGATCCAGAACGAACAGCTGGGTCACCACCACATCATCGACGCCATCGAAAAAATGAAGCGCCCGTTCAAAAGGGACTGGCTGGGGTGCGCGGAAAAAATGTATTAAta                                                                                >  2:1127850/1‑139 (MQ=255)                                   ctgctgGCAGGTTACGGCGGGCGGTGTGTAGGGATCCAGAAAGAAAAGCTGGGTCACCACGACATCATCGACGCTATCCAAAAAAAGAAGCGCCCGTTCAAAGGTGACTGGGTGGGCTGCGCGGAAAAAATGTATTAAta                                                                                >  2:872262/1‑139 (MQ=255)                                     gctgGCAGGTTACGGCGGTCGTTGTGTAGGGATCCCGAACGAACAACTGGGTCACCACGACATCATCGACGCCATCGAAAAAATGAAGCGCCCCGTCAAAAGGGAATGGCTGGACTGCGCGGAAAAAAAGTATTAATTAt                                                                              >  2:649553/1‑140 (MQ=255)                                     gctgGCAGGTTACGGCGGTCGGTGTTTAGGGATCCCCCACCAACAGCGCGTTCACCCCGACACCATCCATGCTCTTCAAAACATGAAAAGCCCGTTCCAAACGGACTGGCTGGCCTGCGCGGAAAAAACGTCTTaactgg                                                                              >  2:829817/1‑136 (MQ=255)                                       tgGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGAttt                                                                            <  2:854325/140‑1 (MQ=255)                                             gTTACGGCGGTCGTTGTGTAGGGATCCAGAACGAACAGCTGGGTTACCACGACATCATCGCCGCTATCGAAAACATGAAACGCCCGTTCCAAAGGGAATGGCTGGGCTGCGCGAAAAAACTGTATTAATGATTTTGGgaa                                                                      >  2:836124/1‑140 (MQ=255)                                                     ggTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGa                                                              <  1:836124/140‑1 (MQ=255)                                                       tCGTTGTGTAGGGATCCAGAACGAACAGCTGGGTCACCACGACATCATCGACGCTATCGAAAAAATGAAGCGCCCGTTCAAAGGGGACTGGCTGGGCTGCGCGAAAAAAATGTATTAATGATTTCGGAAAAAAGCAGaat                                                            >  2:419377/1‑138 (MQ=255)                                                                  gTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCtt                                                        <  2:1130733/133‑1 (MQ=255)                                                                  gTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCtt                                                        >  1:1130733/1‑133 (MQ=255)                                                                  gTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCtt                                                        <  2:1085784/133‑1 (MQ=255)                                                                  gTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCtt                                                        >  1:1085784/1‑133 (MQ=255)                                                                  gTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTg                                                 <  1:585704/140‑1 (MQ=255)                                                                    aTCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGaa                                               >  1:190694/1‑140 (MQ=255)                                                                    aTCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGaa                                               >  1:731399/1‑140 (MQ=255)                                                                    aTCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGaa                                               >  1:972646/1‑140 (MQ=255)                                                                     tCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGaaa                                              >  2:1010555/1‑140 (MQ=255)                                                                     tCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGaaa                                              >  2:1315190/1‑140 (MQ=255)                                                                       cAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTg                                                 >  2:298843/1‑135 (MQ=255)                                                                       cAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTg                                                 <  1:298843/135‑1 (MQ=255)                                                                       cAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAAcc                                            >  1:877616/1‑140 (MQ=255)                                                                         gaacgaacAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGa                                          >  1:1342673/1‑140 (MQ=255)                                                                          aacgaacAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGAt                                         >  1:237476/1‑140 (MQ=255)                                                                           acgaacAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATg                                        >  2:679861/1‑140 (MQ=255)                                                                               acAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAg                                    >  1:618950/1‑140 (MQ=255)                                                                                cAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTATATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGa                                   <  2:237476/140‑1 (MQ=255)                                                                                           ccacGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGc                        >  1:1141045/1‑140 (MQ=255)                                                                                             acGACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCt                      >  1:440300/1‑140 (MQ=255)                                                                                               gACATCATCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGa                    <  2:1342673/140‑1 (MQ=255)                                                                                                 catcatCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCa                               <  1:1250584/127‑1 (MQ=255)                                                                                                 catcatCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCa                               >  2:1250584/1‑127 (MQ=255)                                                                                                 catcatCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATg                  >  2:828176/1‑140 (MQ=255)                                                                                                 catcatCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATg                  >  2:125390/1‑140 (MQ=255)                                                                                                 catcatCGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATg                  >  2:1352283/1‑140 (MQ=255)                                                                                                   tcatcGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGGCTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATgcg                <  1:1127850/140‑1 (MQ=255)                                                                                                   tcatcGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATgcg                <  1:872262/140‑1 (MQ=255)                                                                                                   tcatcGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATgcg                <  1:1167445/140‑1 (MQ=255)                                                                                                    catcGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATgcgc               >  1:754467/1‑140 (MQ=255)                                                                                                      tcGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGcgctt             <  2:1141045/140‑1 (MQ=255)                                                                                                      tcGACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGcgctt             <  2:877616/140‑1 (MQ=255)                                                                                                        gACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGcgcttcg           <  2:190694/140‑1 (MQ=255)                                                                                                        gACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGcgcttcg           <  1:679861/140‑1 (MQ=255)                                                                                                        gACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGcgcttcg           <  1:65830/140‑1 (MQ=255)                                                                                                        gACGCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGcgcttcg           <  2:972646/140‑1 (MQ=255)                                                                                                           gCTATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGcgcttcgctt        >  1:995569/1‑140 (MQ=255)                                                                                                             tATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGcgcttcg           >  1:1241940/1‑135 (MQ=255)                                                                                                             tATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTAt      <  1:828176/140‑1 (MQ=255)                                                                                                             tATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTAt      <  1:649553/140‑1 (MQ=255)                                                                                                             tATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTAt      <  1:1315190/140‑1 (MQ=255)                                                                                                             tATCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATAAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGcgcttcg           <  2:1241940/135‑1 (MQ=255)                                                                                                              aTCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATc     <  2:440300/140‑1 (MQ=255)                                                                                                              aTCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATc     >  1:955794/1‑140 (MQ=255)                                                                                                               tCGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATCa    >  2:138134/1‑140 (MQ=255)                                                                                                                cGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATc     <  1:97990/138‑1 (MQ=255)                                                                                                                cGAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATc     >  2:97990/1‑138 (MQ=255)                                                                                                                 gAAAACATGAAGCGCCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATCAgg  <  2:955794/140‑1 (MQ=255)                                                                                                                               |                                                                                                                              GGCTTCCCGTATGGGCGCTTACGCTATCGATCTGCTGCTGGCAGGTTACGGCGGTCGTTGTGTAGGTATCCAGAACGAACAGCTGGTTCACCACGACATCATCGACGCTATCGAAAACATGAAGCGTCCGTTCAAAGGTGACTGGCTGGACTGCGCGAAAAAACTGTATTAATGATTTCGGAAAAAGGCAGATTCCTTTACCCTGAAACCGATGACAGAAGCAAAAATGCCTGATGCGCTTCGCTTATCAGG  >  NC_000913/4108343‑4108594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑