BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,163,366	A→G	intergenic (‑96/‑273)	trmA ← / → btuB	tRNA m(5)U54 methyltransferase/cobalamin/cobinamide outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,163,366	0	A	G	100.0%	422.6 / NA	116	intergenic (‑96/‑273)	trmA/btuB	tRNA m(5)U54 methyltransferase/cobalamin/cobinamide outer membrane transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (64/52); total (64/52)

Only 100 of 117 total aligned reads displayed.

CTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGACAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGT  >  NC_000913/4163229‑4163501

cTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCa                                                                                                                                       >  1:1368732/1‑140 (MQ=255)

 tGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAg                                                                                                                                      <  1:643814/140‑1 (MQ=255)

   cgcTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTa                                                                                                                                    <  2:120513/140‑1 (MQ=255)

          tACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGGAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGtt                                                                                                                             <  2:663956/140‑1 (MQ=255)

           aCTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTa                                                                                                                            >  1:902225/1‑140 (MQ=255)

           aCTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTa                                                                                                                            <  1:624237/140‑1 (MQ=255)

            ctgttctgttGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTAc                                                                                                                           <  2:1368732/140‑1 (MQ=255)

                tctgttGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAAcc                                                                                                                       >  2:667803/1‑140 (MQ=255)

                  tgttGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTg                                                                                                                     >  1:229151/1‑140 (MQ=255)

                  tgttGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTg                                                                                                                     >  1:1337862/1‑140 (MQ=255)

                    ttGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTa                                                                                                                   >  2:1010971/1‑140 (MQ=255)

                       gAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCa                                                                                                                >  1:196442/1‑140 (MQ=255)

                       gAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATAGCTCATCAGATGTCCAGGTCTTGATGAGTTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCa                                                                                                                >  2:1277164/1‑140 (MQ=255)

                         aGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAAc                                                                                                                        <  1:238320/130‑1 (MQ=255)

                         aGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAAc                                                                                                                        >  2:238320/1‑130 (MQ=255)

                         aGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATc                                                                                                              >  1:1346007/1‑140 (MQ=255)

                         aGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATc                                                                                                              >  2:1410817/1‑140 (MQ=255)

                            tgtTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCa                                                                                                            >  1:1220317/1‑139 (MQ=255)

                            tgtTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCa                                                                                                            <  2:1220317/139‑1 (MQ=255)

                            tgtTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCa                                                                                                            >  1:288406/1‑139 (MQ=255)

                            tgtTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCAc                                                                                                           >  1:179725/1‑140 (MQ=255)

                              ttCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACtt                                                                                                         >  2:1128287/1‑140 (MQ=255)

                              ttCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACtt                                                                                                         >  1:1061282/1‑140 (MQ=255)

                                 gggggTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGcc                                                                                                      <  1:1000096/140‑1 (MQ=255)

                                  ggggTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCg                                                                                                     <  1:924910/140‑1 (MQ=255)

                                      tCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCa                                                                                                            >  1:1027377/1‑129 (MQ=255)

                                      tCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCa                                                                                                            <  2:1027377/129‑1 (MQ=255)

                                      tCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCg                                                                                                     <  1:577531/136‑1 (MQ=255)

                                      tCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCg                                                                                                     >  2:577531/1‑136 (MQ=255)

                                      tCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTcc                                                                                                 >  1:800894/1‑140 (MQ=255)

                                      tCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACATCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTcc                                                                                                 <  1:1119255/140‑1 (MQ=255)

                                       cATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAACTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCt                                                                                                <  1:1247992/140‑1 (MQ=255)

                                        atgatgTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCtg                                                                                               >  1:149119/1‑140 (MQ=255)

                                        atgatgTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCtg                                                                                               >  2:461794/1‑140 (MQ=255)

                                        atgatgTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCtg                                                                                               <  2:1061282/140‑1 (MQ=255)

                                        atgatgTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCtg                                                                                               >  2:557303/1‑140 (MQ=255)

                                        atgatgTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCtg                                                                                               >  1:1382194/1‑140 (MQ=255)

                                         tgatgTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCtgt                                                                                              <  1:832/140‑1 (MQ=255)

                                          gatgTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCtgtg                                                                                             <  1:1196866/140‑1 (MQ=255)

                                          gatgTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCtgtg                                                                                             <  2:850618/140‑1 (MQ=255)

                                          gatgTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCtgtg                                                                                             <  1:288365/140‑1 (MQ=255)

                                             gTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGt                                                                                          <  1:1092591/140‑1 (MQ=255)

                                               aTCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCGGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTa                                                                                        <  2:1192817/140‑1 (MQ=255)

                                               aTCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCt                                                                                                >  1:424486/1‑132 (MQ=255)

                                               aTCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCt                                                                                                <  2:424486/132‑1 (MQ=255)

                                               aTCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTa                                                                                        <  2:902225/140‑1 (MQ=255)

                                               aTCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTa                                                                                        >  2:1304666/1‑140 (MQ=255)

                                               aTCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTa                                                                                        <  2:1346007/140‑1 (MQ=255)

                                               aTCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTa                                                                                        <  2:229151/140‑1 (MQ=255)

                                               aTCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTa                                                                                        >  1:1005475/1‑140 (MQ=255)

                                                tCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTaa                                                                                       >  2:196671/1‑140 (MQ=255)

                                                tCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTaa                                                                                       >  1:565023/1‑140 (MQ=255)

                                                 ccTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAAt                                                                                      <  2:800894/140‑1 (MQ=255)

                                                   tGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAg                                                                                    >  2:279398/1‑140 (MQ=255)

                                                     aaaaaTTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAggg                                                                                  >  1:291262/1‑140 (MQ=255)

                                                     aaaaaTTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAggg                                                                                  >  1:443988/1‑140 (MQ=255)

                                                             aaGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGt                                                                          >  2:125824/1‑140 (MQ=255)

                                                                         aTTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAg                                                              >  1:1318630/1‑140 (MQ=255)

                                                                                 gATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGAcgcg                                                      <  2:38614/140‑1 (MQ=255)

                                                                                   ttGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCa                                                    <  2:1311158/140‑1 (MQ=255)

                                                                                   ttGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCa                                                    >  1:458435/1‑140 (MQ=255)

                                                                                   ttGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCa                                                    >  2:1373652/1‑140 (MQ=255)

                                                                                   ttGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCa                                                    <  1:196671/140‑1 (MQ=255)

                                                                                    tGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAg                                                   <  1:667803/140‑1 (MQ=255)

                                                                                    tGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAg                                                   <  1:1373652/140‑1 (MQ=255)

                                                                                    tGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAg                                                   <  1:1410817/140‑1 (MQ=255)

                                                                                    tGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAg                                                   <  1:171065/140‑1 (MQ=255)

                                                                                    tGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAg                                                   <  2:1382194/140‑1 (MQ=255)

                                                                                    tGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAg                                                   >  2:206225/1‑140 (MQ=255)

                                                                                     gCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGc                                                  >  2:11757/1‑140 (MQ=255)

                                                                                     gCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGc                                                  >  1:125678/1‑140 (MQ=255)

                                                                                     gCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGc                                                  >  1:747795/1‑140 (MQ=255)

                                                                                      cTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCg                                                 >  2:745370/1‑140 (MQ=255)

                                                                                       tcatcaGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGAcgcg                                                      <  1:1129977/134‑1 (MQ=255)

                                                                                       tcatcaGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGAcgcg                                                      >  2:1129977/1‑134 (MQ=255)

                                                                                       tcatcaGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGc                                                  >  2:474745/1‑138 (MQ=255)

                                                                                       tcatcaGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGc                                                  <  1:474745/138‑1 (MQ=255)

                                                                                       tcatcaGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCgg                                                <  2:179725/140‑1 (MQ=255)

                                                                                          tcaGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTaa                                             >  1:795606/1‑140 (MQ=255)

                                                                                              aTGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGaa                                         >  2:321456/1‑140 (MQ=255)

                                                                                                gTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAg                                       >  2:892800/1‑140 (MQ=255)

                                                                                                gTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAg                                       >  2:603917/1‑140 (MQ=255)

                                                                                                gTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAg                                       >  2:894128/1‑140 (MQ=255)

                                                                                                 tCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAgg                                      >  1:470667/1‑140 (MQ=255)

                                                                                                   cAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTg                                    <  1:892800/140‑1 (MQ=255)

                                                                                                   cAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTg                                    >  1:751400/1‑140 (MQ=255)

                                                                                                     gATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCg                                  <  2:1409726/140‑1 (MQ=255)

                                                                                                           gatgaATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGAtt                            >  1:503640/1‑140 (MQ=255)

                                                                                                           gatgaATTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGAtt                            >  2:1115322/1‑140 (MQ=255)

                                                                                                               aaTTCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGt                        <  2:747795/140‑1 (MQ=255)

                                                                                                                  tCCTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTAt                     <  2:503640/140‑1 (MQ=255)

                                                                                                                   ccTATTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATg                    <  2:443988/140‑1 (MQ=255)

                                                                                                                      aTTTGTGAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCgg                 >  2:557351/1‑140 (MQ=255)

                                                                                                                           tgAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACt            >  2:503979/1‑140 (MQ=255)

                                                                                                                           tgAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACt            >  2:466716/1‑140 (MQ=255)

                                                                                                                           tgAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACt            >  1:1193746/1‑140 (MQ=255)

                                                                                                                            gAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTg           >  1:57145/1‑140 (MQ=255)

                                                                                                                            gAGCTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTg           <  2:57145/140‑1 (MQ=255)

                                                                                                                               cTACGTCTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCa        >  2:1107303/1‑140 (MQ=255)

                                                                                                                                     cTGGGCAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGt  <  1:296459/140‑1 (MQ=255)

CTGCGCTTCATACTGTTCTGTTGGAAGGTGTTCGGGGGTCATGATGTATCCTGAAAAATTAAAGTACGCGGCGATTGTAGGGATTGCTCATCAGATGTCCAGATCTTGATGAATTCCTATTTGTGAGCTACGTCTGGACAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGT  >  NC_000913/4163229‑4163501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`