Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,326,695	T→C	T14A (ACC→GCC)	yjdM ←	conserved protein YjdM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,326,695	0	T	C	100.0%	189.4 / NA	53	T14A (ACC→GCC)	yjdM	conserved protein YjdM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/20);  total (33/20)

GGTAACGCTGTCGCCGTCAGCCAGCAGATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGTGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGG  >  NC_000913/4326558‑4326830                                                                                                                                          |                                                                                                                                        ggTAACGCTGTCGCCGTCAGCCAGCAGATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGgcgt                                                                                                                                       >  2:544285/1‑140 (MQ=255)         tGTCGCCGTCAGCCAGCAGATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTAtt                                                                                                                                    >  2:631895/1‑135 (MQ=255)         tGTCGCCGTCAGCCAGCAGATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTAtt                                                                                                                                    <  1:631895/135‑1 (MQ=255)             gCCGTCAGCCAGCAGATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGtt                                                                                                                             >  1:158986/1‑138 (MQ=255)             gCCGTCAGCCAGCAGATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGtt                                                                                                                             <  2:158986/138‑1 (MQ=255)             gCCGTCAGCCAGCAGATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGc                                                                                                                           >  2:910267/1‑140 (MQ=255)             gCCGTCAGCCAGCAGATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGc                                                                                                                           >  2:1245148/1‑140 (MQ=255)                     ccagcAGATTGTCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTggg                                                                                                                   <  1:228333/140‑1 (MQ=255)                     ccagcAGATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCGGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTggg                                                                                                                   <  2:1204633/140‑1 (MQ=255)                        gcagATTGCCTTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAg                                                                                                                <  2:1182364/140‑1 (MQ=255)                         cagATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAgt                                                                                                               >  1:193074/1‑140 (MQ=255)                          agATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAgtg                                                                                                              <  2:193074/140‑1 (MQ=255)                          agATTGCCGTTACCATCTTTAACGAGCAGCTCGTCGCTTCCCTGTGCGGGTCTTGCGTCGTTCCATTCGTGGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAgtg                                                                                                              <  2:219765/140‑1 (MQ=255)                                      gCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACAt                                                                                                  >  1:1190621/1‑140 (MQ=255)                                           tttAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGa                                                                                             >  1:1230793/1‑140 (MQ=255)                                             tAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGAtt                                                                                           >  2:1142563/1‑140 (MQ=255)                                                cGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTcc                                                                                        >  1:566476/1‑140 (MQ=255)                                                 gATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCt                                                                                       <  2:566476/140‑1 (MQ=255)                                                  aTCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTc                                                                                      >  1:286147/1‑140 (MQ=255)                                                  aTCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTc                                                                                      >  1:593924/1‑140 (MQ=255)                                                   tCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCa                                                                                     <  2:1190621/140‑1 (MQ=255)                                                    cAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCaa                                                                                    >  1:1045918/1‑140 (MQ=255)                                                    cAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCaa                                                                                    >  2:41378/1‑140 (MQ=255)                                                    cAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCaa                                                                                    >  1:866219/1‑140 (MQ=255)                                                     aGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCaaa                                                                                   >  1:1118760/1‑140 (MQ=255)                                                     aGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCaaa                                                                                   >  1:114432/1‑140 (MQ=255)                                                       cTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGCAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAg                                                                                 >  1:226507/1‑140 (MQ=255)                                                         cgtccttttCCGGTGCAGGTTCTGCTTCGTCCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTc                                                                               <  2:1208940/134‑1 (MQ=255)                                                                     gtgCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCaaaa                                                                   >  2:398335/1‑140 (MQ=255)                                                                           gTTCTGCGTCGTTCCATTNNTAGGCNCANTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGNGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCa                                                             >  2:445823/1‑140 (MQ=255)                                                                           gTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCa                                                             >  2:675313/1‑140 (MQ=255)                                                                           gTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCa                                                             >  2:1369345/1‑140 (MQ=255)                                                                             tCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCaaa                                                           >  2:1271331/1‑140 (MQ=255)                                                                                      ttCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAg                                                  >  2:163892/1‑140 (MQ=255)                                                                                       tCCATTCGTAGGCACATCCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGa                                                 <  1:405178/140‑1 (MQ=255)                                                                                                aGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGaaa                                        >  2:689698/1‑140 (MQ=255)                                                                                                     caTTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGc                                   <  1:910267/140‑1 (MQ=255)                                                                                                       ttCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCg                                 <  1:689698/140‑1 (MQ=255)                                                                                                       ttCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCg                                 <  2:866219/140‑1 (MQ=255)                                                                                                        tCCGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGc                                >  2:512060/1‑140 (MQ=255)                                                                                                          cGGGCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCaa                              <  1:398335/140‑1 (MQ=255)                                                                                                            ggCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAAtt                            >  1:680692/1‑140 (MQ=255)                                                                                                             gCAGATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATta                           <  1:41378/140‑1 (MQ=255)                                                                                                                gATGTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATac                        >  1:487930/1‑140 (MQ=255)                                                                                                                   gTACATGCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATAcaca                     <  1:675313/140‑1 (MQ=255)                                                                                                                         gCCGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATcc               >  1:383907/1‑140 (MQ=255)                                                                                                                          ccGTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAGTccc              >  2:1314780/1‑140 (MQ=255)                                                                                                                            gTTATCTTCGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTg            >  2:624756/1‑140 (MQ=255)                                                                                                                                    cGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGt         >  2:965635/1‑135 (MQ=255)                                                                                                                                    cGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGt         <  1:965635/135‑1 (MQ=255)                                                                                                                                    cGTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTc    <  1:512060/140‑1 (MQ=255)                                                                                                                                     gTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCg   >  2:721027/1‑140 (MQ=255)                                                                                                                                     gTAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCg   >  1:1098543/1‑140 (MQ=255)                                                                                                                                      tAGGCGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCgg  <  1:1314780/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GGTAACGCTGTCGCCGTCAGCCAGCAGATTGCCGTTAGCATCTTTAACGATCAGCTCGTCGCTTTCCTGTGCAGGTTCTGCGTCGTTCCATTCGTAGGCACATTCCGGGCAGATGTACATGCCGTTATCTTCGTAGGTGTATTCGGAGTTGCATTTTGGGCAGTGTGGTAATGACATGTGGATTTCCTCAAAAGTCAGCAGGGCAAAAGCGCCAAAAAACGGCAGATTGCCGAAAAAGGCCGCAATTATACACAAAATCCCTGAGTTTGTCGG  >  NC_000913/4326558‑4326830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑