Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,571,858	G→A	Q36Q (CAG→CAA)	yjiS →	DUF1127 domain‑containing protein YjiS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,571,858	0	G	A	100.0%	297.4 / NA	83	Q36Q (CAG→CAA)	yjiS	DUF1127 domain‑containing protein YjiS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/55);  total (28/55)

CTCTGAAAGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAGCAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGATTTATATATAATAAAACGTTTTT  >  NC_000913/4571727‑4571951                                                                                                                                    |                                                                                               ctctGAAAGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAg                                                                                        >  1:261408/1‑140 (MQ=255)  tctGAAAGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGt                                                                                       >  2:821145/1‑140 (MQ=255)   ctGAAAGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGtg                                                                                      >  2:1253700/1‑140 (MQ=255)    tGAAAGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGtga                                                                                     >  1:1248385/1‑140 (MQ=255)    tGAAAGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCCGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGGGtga                                                                                     >  2:1313772/1‑140 (MQ=255)     gAAAGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGtgat                                                                                    <  1:1253700/140‑1 (MQ=255)      aaaGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGtgatg                                                                                   <  2:1057888/140‑1 (MQ=255)       aaGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGtgatga                                                                                  <  2:1164708/140‑1 (MQ=255)        aGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAg                                                                                 <  1:53126/140‑1 (MQ=255)        aGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAg                                                                                 <  1:82748/140‑1 (MQ=255)        aGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAg                                                                                 <  1:168866/140‑1 (MQ=255)          aTGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCg                                                                               >  2:51135/1‑140 (MQ=255)           tGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCgg                                                                              >  2:1285662/1‑140 (MQ=255)           tGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCgg                                                                              <  2:35807/140‑1 (MQ=255)           tGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCgg                                                                              <  2:974873/140‑1 (MQ=255)            gaggagGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGt                                                                             >  1:942089/1‑140 (MQ=255)             aggaggTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGtg                                                                                      >  1:1021299/1‑130 (MQ=255)             aggaggTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGtg                                                                                      <  2:1021299/130‑1 (MQ=255)             aggaggTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGtt                                                                            >  1:919048/1‑140 (MQ=255)                aggTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGaa                                                                         >  2:1101828/1‑140 (MQ=255)                  gTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGACGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAgg                                                                       >  2:398626/1‑140 (MQ=255)                    aGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAg                                                                                 >  1:571416/1‑128 (MQ=255)                    aGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAg                                                                                 <  2:571416/128‑1 (MQ=255)                    aGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGat                                                                     >  2:740183/1‑140 (MQ=255)                     gCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGata                                                                    <  2:340508/140‑1 (MQ=255)                       aGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATc                                                                  <  1:1191304/140‑1 (MQ=255)                         aTGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑gg                                                               <  2:527484/140‑1 (MQ=255)                          tGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑ggg                                                              >  1:969976/1‑140 (MQ=255)                          tGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑ggg                                                              <  2:13297/140‑1 (MQ=255)                          tGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑ggg                                                              <  2:1248385/140‑1 (MQ=255)                          tGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑ggg                                                              <  1:161238/140‑1 (MQ=255)                          tGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTCGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑ggg                                                              <  2:1325527/140‑1 (MQ=255)                           ggAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGt                                                             <  2:439988/140‑1 (MQ=255)                           ggAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGt                                                             >  2:77994/1‑140 (MQ=255)                           ggAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGt                                                             <  1:1285662/140‑1 (MQ=255)                             aaTTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑ggggtt                                                           >  1:731289/1‑137 (MQ=255)                             aaTTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTa                                                           <  1:685020/140‑1 (MQ=255)                               tttCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑ggg                                                              <  2:1288905/135‑1 (MQ=255)                               tttCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑ggg                                                              >  1:1288905/1‑135 (MQ=255)                                  cACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAg                                                      <  1:114621/140‑1 (MQ=255)                                    cGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAggg                                                    <  1:482748/140‑1 (MQ=255)                                    cGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAggg                                                    <  2:1197118/140‑1 (MQ=255)                                    cGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAggg                                                    <  1:1091763/140‑1 (MQ=255)                                    cGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAggg                                                    <  1:642931/140‑1 (MQ=255)                                    cGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAACGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAggg                                                    <  1:1247880/140‑1 (MQ=255)                                     gAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGgga                                                   <  1:77994/140‑1 (MQ=255)                                       aaaCAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTa                                                           >  1:1312375/1‑130 (MQ=255)                                       aaaCAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTa                                                           <  2:1312375/130‑1 (MQ=255)                                       aaaCAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGggagg                                                 <  2:153385/140‑1 (MQ=255)                                            gagCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGAtgtg                                            <  1:1171888/140‑1 (MQ=255)                                            gagCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGAtgtg                                            <  2:919048/140‑1 (MQ=255)                                            gagCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGACGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGAtgtg                                            <  1:398626/140‑1 (MQ=255)                                             aggtAAAGGGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGGGGTGAGGCGGTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGAAGGGAGGATGTgg                                           <  2:12800/137‑1 (MQ=255)                                                 aaaGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTg                                       <  1:740183/140‑1 (MQ=255)                                                   aGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAg                                     <  2:969976/140‑1 (MQ=255)                                                   aGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAg                                     <  1:1138322/140‑1 (MQ=255)                                                   aGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAg                                     <  2:919108/140‑1 (MQ=255)                                                     cgcCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAggg                                   <  2:809527/140‑1 (MQ=255)                                                          tttATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGat                              >  2:1381573/1‑140 (MQ=255)                                                          tttATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGat                              >  1:731403/1‑140 (MQ=255)                                                          tgtATCGGCCTGGTACAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATTCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGat                              <  2:876635/138‑1 (MQ=255)                                                           ttATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGata                             <  2:731403/140‑1 (MQ=255)                                                           ttATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGata                             <  2:942089/140‑1 (MQ=255)                                                           ttATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGata                             <  1:1101828/140‑1 (MQ=255)                                                           ttATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGata                             <  1:51135/140‑1 (MQ=255)                                                           ttATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGata                             <  2:261408/140‑1 (MQ=255)                                                           ttATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGata                             <  1:821145/140‑1 (MQ=255)                                                           ttATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGata                             <  2:165417/140‑1 (MQ=255)                                                           ttATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGata                             <  1:323101/140‑1 (MQ=255)                                                            tATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATCGGGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGata                             <  2:731289/140‑1 (MQ=255)                                                                ggCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGAt                        <  2:358270/140‑1 (MQ=255)                                                                ggCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGAt                        <  2:45995/140‑1 (MQ=255)                                                                ggCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGAt                        <  2:30693/140‑1 (MQ=255)                                                                  ccTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAgg                                    <  1:1072995/126‑1 (MQ=255)                                                                  ccTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAgg                                    >  2:1072995/1‑126 (MQ=255)                                                                  ccTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTT‑AAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAgg                                    >  2:89851/1‑125 (MQ=255)                                                                  ccTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTT‑AAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAgg                                    <  1:89851/125‑1 (MQ=255)                                                                    tGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGata                             >  1:1223421/1‑131 (MQ=255)                                                                    tGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGata                             <  2:1223421/131‑1 (MQ=255)                                                                            ctctGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGATTTATATataata            >  1:934781/1‑140 (MQ=255)                                                                            ctctGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGATTTATATataata            >  1:899656/1‑140 (MQ=255)                                                                                ggCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAACAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGATTTATATATAATAAAAc        >  2:526966/1‑140 (MQ=255)                                                                                      cGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGCTACAACAGATGGGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGGTGTGGAGTGAGGGGGGATATAGATTTATATATAATAAAACGttttt  >  2:221897/1‑140 (MQ=255)                                                                                                                                    |                                                                                               CTCTGAAAGATGAGGAGGTAGCAGATGGAATTTCACGAAAACAGAGCTAAAGCGCCGTTTATCGGCCTGGTGCAACTCTGGCAGGCGGTGAGGCGTTGGCGGCGGCAAATGCAGACCCGACGCGTGTTACAGCAGATGAGTGATGAGCGGTTGAAGGATATC‑GGGTTACGCAGGGAGGATGTGGAGTGAGGGGGGATATAGATTTATATATAATAAAACGTTTTT  >  NC_000913/4571727‑4571951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑