Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	360,285	T→C	G98G (GGT→GGC)	cynX →	cyanate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	360,285	0	T	C	100.0%	198.9 / NA	56	G98G (GGT→GGC)	cynX	cyanate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/29);  total (27/29)

AGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTGCCGCCATA  >  NC_000913/360157‑360417                                                                                                                                 |                                                                                                                                     aGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGAt                                                                                                                           <  1:562429/140‑1 (MQ=255) aGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGAt                                                                                                                           <  1:874972/140‑1 (MQ=255)     ggCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGc                                                                                                                       >  1:560326/1‑140 (MQ=255)     ggCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGc                                                                                                                       >  1:881463/1‑140 (MQ=255)          catcaGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGcatcat                                                                                                                  >  1:761865/1‑140 (MQ=255)                    tCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTg                                                                                                        <  2:722371/140‑1 (MQ=255)                    tCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTg                                                                                                        <  1:240241/140‑1 (MQ=255)                    tCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTg                                                                                                        <  1:886531/140‑1 (MQ=255)                     cAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGa                                                                                                       >  2:228846/1‑140 (MQ=255)                      aGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGAt                                                                                                      <  2:560326/140‑1 (MQ=255)                        cGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGc                                                                                                    >  1:887480/1‑140 (MQ=255)                            cGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAg                                                                                                              <  2:166351/126‑1 (MQ=255)                            cGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAg                                                                                                              >  1:166351/1‑126 (MQ=255)                             gTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCg                                                                                               >  1:891254/1‑140 (MQ=255)                             gTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCg                                                                                               >  2:1192784/1‑140 (MQ=255)                              tCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCgg                                                                                              <  2:887480/140‑1 (MQ=255)                                         ccATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATg                                                                                                     <  1:272713/122‑1 (MQ=255)                                         ccATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATg                                                                                                     >  2:272713/1‑122 (MQ=255)                                          cATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAAcggc                                                                                  <  1:782563/140‑1 (MQ=255)                                              aGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGtt                                                                              >  1:939716/1‑140 (MQ=255)                                                       cTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAgcg                                                                     >  2:637218/1‑140 (MQ=255)                                                       cTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAgcg                                                                     <  1:39665/140‑1 (MQ=255)                                                         gATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCa                                                                   <  2:41737/140‑1 (MQ=255)                                                          aTTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCAc                                                                  >  2:344285/1‑140 (MQ=255)                                                            tGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGc                                                                >  2:1021466/1‑140 (MQ=255)                                                            tGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGc                                                                <  1:1192784/140‑1 (MQ=255)                                                              ccGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCa                                                                   <  1:228348/135‑1 (MQ=255)                                                              ccGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCa                                                                   >  2:228348/1‑135 (MQ=255)                                                                gTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACt                                                            <  2:881463/140‑1 (MQ=255)                                                                gTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACt                                                            <  2:85661/140‑1 (MQ=255)                                                                    gTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTg                                                        >  1:435115/1‑140 (MQ=255)                                                                       cATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATg                                                     <  1:24008/140‑1 (MQ=255)                                                                       cATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATg                                                     <  1:344285/140‑1 (MQ=255)                                                                        aTTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTcagcaggg                                                                     >  1:392665/1‑121 (MQ=255)                                                                        aTTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAgcg                                                                     <  2:392665/123‑1 (MQ=255)                                                                        aTTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATgg                                                    >  1:227213/1‑140 (MQ=255)                                                                              gCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTgg                                                          <  2:183326/128‑1 (MQ=255)                                                                              gCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTgg                                                          >  1:183326/1‑128 (MQ=255)                                                                               cGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAGACGGCGGTTTCAACAGCGCACGCCACTGGGGGTGGGGccggg                                             >  2:1329498/1‑136 (MQ=255)                                                                               cGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCtgtg                                             <  2:939716/140‑1 (MQ=255)                                                                               cGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCtgtg                                             >  1:794367/1‑140 (MQ=255)                                                                                  gAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTc                                          <  1:453915/140‑1 (MQ=255)                                                                                       ttACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCgg                                     <  2:794367/140‑1 (MQ=255)                                                                                        tACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGTCTTCGGTGATTAAACGGGGGTTTTATCAGCGCACCCCACTGGGGATTGGGGTGTGGGCCGCggg                                    >  2:335058/1‑139 (MQ=255)                                                                                         aCCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGTCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGct                                   <  1:335058/140‑1 (MQ=255)                                                                                          cccGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCcg                                        >  2:24044/1‑134 (MQ=255)                                                                                          cccGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCcg                                        <  1:24044/134‑1 (MQ=255)                                                                                                 aGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATggg                           <  2:891254/140‑1 (MQ=255)                                                                                                        tgctgctTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGt                    <  1:867761/140‑1 (MQ=255)                                                                                                            gctTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGc                <  2:227213/140‑1 (MQ=255)                                                                                                             ctTAGCAGCGCACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTTAGCAGCGCACGCCCCTGGGGGTGGGGGTGTGGGCCGCGGCTCTTATGgggggggggggggt               >  2:1396751/1‑138 (MQ=255)                                                                                                                    gcgcACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCCCTGGGGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCCTGGTgcc        >  2:895248/1‑140 (MQ=255)                                                                                                                    gcgcACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGGGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTgcc        >  2:186723/1‑140 (MQ=255)                                                                                                                     cgcACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTgccg       >  1:1329165/1‑140 (MQ=255)                                                                                                                       cACTGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCCCGCCACTGGGGGTGGGGGTGGGGGCCGCGGCTCTGATTGGCGGCGGTGGGCTTGGTgccgcc     >  2:424045/1‑140 (MQ=255)                                                                                                                          tGCTTGGCGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTGCCGCCATa  <  1:1342891/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                     AGCTGGCTTCATCAGCATGTCAGCGAACGTCGCAGTGTCGCCATCAGTCTGTTGCTGATTGCCGTCGGTGCATTGATGCGTGAGCTTTACCCGCAAAGTGCGCTGCTGCTTAGCAGCGCACTGCTTGGTGGGGTGGGGATCGGCATCATTCAGGCGGTGATGCCTTCGGTGATTAAACGGCGGTTTCAGCAGCGCACGCCACTGGTGATGGGGCTGTGGTCCGCGGCTCTGATGGGCGGCGGTGGGCTTGGTGCCGCCATA  >  NC_000913/360157‑360417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑