Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,597,472	T→C	N657S (AAC→AGC)	opgB ←	phosphoglycerol transferase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,597,472	0	T	C	100.0%	202.0 / NA	57	N657S (AAC→AGC)	opgB	phosphoglycerol transferase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/30);  total (27/30)

TTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGA  >  NC_000913/4597342‑4597607                                                                                                                                   |                                                                                                                                        ttcATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGc                                                                                                                                <  1:756864/140‑1 (MQ=255)        ccTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCa                                                                                                                         >  1:94038/1‑140 (MQ=255)        ccTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCa                                                                                                                         >  1:406457/1‑140 (MQ=255)        ccTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAAATTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCa                                                                                                                         <  2:995183/140‑1 (MQ=255)          tACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACg                                                                                                                       >  1:165993/1‑140 (MQ=255)           aCGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGa                                                                                                                      >  1:910809/1‑140 (MQ=255)           aCGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGa                                                                                                                      >  1:81077/1‑140 (MQ=255)                 aCCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCgg                                                                                                                <  2:1092329/140‑1 (MQ=255)                  ccGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGa                                                                                                               >  1:358058/1‑140 (MQ=255)                  ccGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGa                                                                                                               >  2:538797/1‑140 (MQ=255)                  ccGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGCCCCCACGACTCCGGa                                                                                                               >  1:683296/1‑140 (MQ=255)                       aTAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGa                                                                                                          >  1:85779/1‑140 (MQ=255)                         aGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGaaa                                                                                                        <  1:478903/140‑1 (MQ=255)                         aGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGCACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGaaa                                                                                                        <  2:406457/140‑1 (MQ=255)                                   gCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTaaa                                                                                              <  1:258815/140‑1 (MQ=255)                                       tATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGt                                                                                          <  1:781213/140‑1 (MQ=255)                                       tATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGCCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGt                                                                                          <  2:683296/140‑1 (MQ=255)                                             cGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCAcc                                                                                    >  2:1073835/1‑140 (MQ=255)                                               tATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACctc                                                                                  <  2:81077/140‑1 (MQ=255)                                                 tGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCtctt                                                                                >  1:362778/1‑140 (MQ=255)                                                 tGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCtctt                                                                                >  2:1006062/1‑140 (MQ=255)                                                   cTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTGAACTGTTTCACCtcttct                                                                              >  1:1153934/1‑140 (MQ=255)                                                   cTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCtcttct                                                                              >  1:223365/1‑140 (MQ=255)                                                       ggCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGgcg                                                                          >  2:1210580/1‑140 (MQ=255)                                                             aaTCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCgg                                                                    <  1:538797/140‑1 (MQ=255)                                                             aaTCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCgg                                                                    <  2:94038/140‑1 (MQ=255)                                                              aTCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGc                                                                   >  2:221496/1‑140 (MQ=255)                                                               tCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCg                                                                  <  2:85779/140‑1 (MQ=255)                                                                    aGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGtt                                                             <  1:221496/140‑1 (MQ=255)                                                                      gTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTaa                                                           >  1:1312675/1‑140 (MQ=255)                                                                      gTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTaa                                                           >  1:1362809/1‑140 (MQ=255)                                                                         aagtttCTTCGGCAGCGGATGCTTGTACTCCATTTTTTCTTCATCGCCTAGCTGCGCGCTGGCCCAGCGGCACCACGACGCCGGACGGCAAATCCCGCTAAACTGTTTCGCCTCTTCTGGCGCACCGGCGTCGTTAAAGa                                                        <  2:877010/137‑1 (MQ=255)                                                                                ttcGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACt                                                 >  1:250047/1‑140 (MQ=255)                                                                                  cGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGt                                               <  1:257653/140‑1 (MQ=255)                                                                                  cGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGt                                               <  2:223365/140‑1 (MQ=255)                                                                                     cAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCgg                                            >  2:1331784/1‑140 (MQ=255)                                                                                     cAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCgg                                            >  2:1098921/1‑140 (MQ=255)                                                                                         ggATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCttt                                        <  2:250047/140‑1 (MQ=255)                                                                                          gATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTg                                       <  1:1210580/140‑1 (MQ=255)                                                                                            tGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTa                                     <  1:710606/140‑1 (MQ=255)                                                                                             gCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAg                                    <  2:165993/140‑1 (MQ=255)                                                                                             gCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAg                                    <  2:358058/140‑1 (MQ=255)                                                                                                  tACTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAat                               >  2:895301/1‑140 (MQ=255)                                                                                                    cTCGATTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAatat                             >  2:720426/1‑140 (MQ=255)                                                                                                        aTTTTTACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGtt                         <  2:116193/140‑1 (MQ=255)                                                                                                          ttttACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTAt                       <  2:1312675/140‑1 (MQ=255)                                                                                                          ttttACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTAt                       <  1:722270/140‑1 (MQ=255)                                                                                                             tACTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATcaa                    <  2:910809/140‑1 (MQ=255)                                                                                                              aCTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATcaac                   <  1:1006062/140‑1 (MQ=255)                                                                                                              aCTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATcaac                   >  2:315876/1‑140 (MQ=255)                                                                                                               cTTCATCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATcaaca                  <  2:245691/140‑1 (MQ=255)                                                                                                                   aTCGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCGGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCt              >  2:1133266/1‑140 (MQ=255)                                                                                                                     cGCCCAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTc            <  2:565797/140‑1 (MQ=255)                                                                                                                       cccAGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAg          <  2:369756/140‑1 (MQ=255)                                                                                                                          aGCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGt       >  2:338399/1‑140 (MQ=255)                                                                                                                           gCTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTa      <  2:505944/140‑1 (MQ=255)                                                                                                                            cTGCGCGCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTAt     <  2:1009052/140‑1 (MQ=255)                                                                                                                               cgcgCTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGa  >  1:115381/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        TTCATTGCCTACGCGTACCGGAATAGGACGGCTGGCGTTATTGCCGTATGCTTTGGCGGTAATCACCAGGTCAAATTTCTTCGGCAGCGGATGCTTGTACTCGATTTTTACTTCATCGCCCAGCTGCGCGTTGGACCAGCGGCCCCACGACTCCGGACGGGAAATCCCGCTAAACTGTTTCACCTCTTCTGGCGCACCGGCGACGTTAAAGATGAAACTGTCGGCTTTGTAGCGAATATCGTTATCAACAATCTTCAGCGTATCGA  >  NC_000913/4597342‑4597607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑