Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,601,309	T→C	D70G (GAC→GGC)	dnaT ←	primosomal protein DnaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,601,309	0	T	C	100.0%	226.1 / NA	64	D70G (GAC→GGC)	dnaT	primosomal protein DnaT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/28);  total (36/28)

CTCTCTTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTT  >  NC_000913/4601176‑4601443                                                                                                                                      |                                                                                                                                       ctctctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCagag                                                                                                                                  <  1:194956/140‑1 (MQ=255) ctctctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCagag                                                                                                                                  <  1:1338525/140‑1 (MQ=255) ctctctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCagag                                                                                                                                  >  2:773503/1‑140 (MQ=255)   ctctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCg                                                                                                                                >  1:1342945/1‑140 (MQ=255)    tctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGa                                                                                                                               >  2:457019/1‑140 (MQ=255)     ctTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGAc                                                                                                                              <  1:601826/140‑1 (MQ=255)       tAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACAt                                                                                                                            <  2:74247/140‑1 (MQ=255)              aCGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTcc                                                                                                                     >  2:118722/1‑140 (MQ=255)               cGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTccc                                                                                                                    >  2:245405/1‑140 (MQ=255)                gCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCg                                                                                                                   >  1:1143702/1‑140 (MQ=255)                gCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCg                                                                                                                   >  1:726641/1‑140 (MQ=255)                gCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCg                                                                                                                   >  2:935890/1‑140 (MQ=255)                    cATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGAcg                                                                                                               >  2:858249/1‑140 (MQ=255)                      tAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGAcgcg                                                                                                             >  1:613684/1‑140 (MQ=255)                      tAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGAcgcg                                                                                                             <  2:1342945/140‑1 (MQ=255)                        gcgcCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGcc                                                                                                           <  1:935890/140‑1 (MQ=255)                         cgccgcCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGGGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCa                                                                                                          >  1:1116787/1‑140 (MQ=255)                                   cGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTttcttc                                                                                                >  2:959059/1‑140 (MQ=255)                                   cGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTttcttc                                                                                                >  1:1385522/1‑140 (MQ=255)                                       tAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAgc                                                                                            <  1:457019/140‑1 (MQ=255)                                          aaTCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGcc                                                                                         <  1:130402/140‑1 (MQ=255)                                                cATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGGGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGt                                                                                   <  2:1116787/140‑1 (MQ=255)                                                   cGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCa                                                                                <  1:118722/140‑1 (MQ=255)                                                    gggTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAg                                                                               <  2:150667/140‑1 (MQ=255)                                                        tGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcag                                                                           <  1:858249/140‑1 (MQ=255)                                                             gTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtg                                                                      <  2:1266609/140‑1 (MQ=255)                                                              tCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgc                                                                     >  2:260485/1‑140 (MQ=255)                                                               ccGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgca                                                                    >  1:1136213/1‑140 (MQ=255)                                                                  gATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCcaggcgtgcaggc                                                                 >  1:1108440/1‑140 (MQ=255)                                                                         ggCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACgg                                                          >  2:521741/1‑140 (MQ=255)                                                                         ggCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACgg                                                          >  1:1113982/1‑140 (MQ=255)                                                                          gCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGc                                                         >  1:84381/1‑140 (MQ=255)                                                                                  ccccATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACACGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACg                                                 <  1:192177/140‑1 (MQ=255)                                                                                        gggTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCgggg                                           >  1:1418465/1‑140 (MQ=255)                                                                                          gTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGGCGGCATAAAACGCCGGGGCa                                         <  1:245405/140‑1 (MQ=255)                                                                                          gTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCa                                         <  1:1075199/140‑1 (MQ=255)                                                                                           tACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCAt                                        <  1:10601/140‑1 (MQ=255)                                                                                           tACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCAt                                        <  2:805241/140‑1 (MQ=255)                                                                                             cAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTg                                      <  2:726641/140‑1 (MQ=255)                                                                                                cAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTa                                   <  1:260485/140‑1 (MQ=255)                                                                                                  aCGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGc                                 >  1:1380675/1‑140 (MQ=255)                                                                                                  aCGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGc                                 <  2:1380675/140‑1 (MQ=255)                                                                                                  aCGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTACCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGc                                 >  2:1132847/1‑140 (MQ=255)                                                                                                   cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCTAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCa                                <  2:1143702/140‑1 (MQ=255)                                                                                                   cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCgggg                                           <  1:502544/129‑1 (MQ=255)                                                                                                   cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCgggg                                           >  2:502544/1‑129 (MQ=255)                                                                                                   cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCa                                >  2:309873/1‑140 (MQ=255)                                                                                                   cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCa                                >  2:510512/1‑140 (MQ=255)                                                                                                   cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCa                                >  1:1415405/1‑140 (MQ=255)                                                                                                   cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCa                                >  2:141716/1‑140 (MQ=255)                                                                                                   cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCa                                >  2:634180/1‑140 (MQ=255)                                                                                                   cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCa                                >  2:701321/1‑140 (MQ=255)                                                                                                   cGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCa                                >  1:928682/1‑140 (MQ=255)                                                                                                    gggAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCgggg                                           >  1:292116/1‑128 (MQ=255)                                                                                                    gggAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCgggg                                           <  2:292116/128‑1 (MQ=255)                                                                                                     ggAGCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCaaa                              >  2:797982/1‑140 (MQ=255)                                                                                                        gCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATAc                           <  2:613684/140‑1 (MQ=255)                                                                                                        gCGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATAc                           <  2:1385522/140‑1 (MQ=255)                                                                                                         cGGCTTGCGGTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACg                          <  1:1132847/140‑1 (MQ=255)                                                                                                                  gTTCCTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACcaca                 <  1:1267311/140‑1 (MQ=255)                                                                                                                     ccTGATAGAGTTGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCg              >  2:1205112/1‑140 (MQ=255)                                                                                                                               ttGATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAACAAATACGGCAACCACACCGCCTTCAGCtt    >  1:1089124/1‑140 (MQ=255)                                                                                                                                 gATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCtttt  >  1:771133/1‑140 (MQ=255)                                                                                                                                 gATCGCCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCtttt  >  1:653928/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       CTCTCTTAGCGCCACGCCCCATAGCGCCGCCAGGCGGATAAAATCGGCATCGGGTTGCCAGTCCGGATACATGGCGAATTTCCCCATGGGTACAGCAACGGGAGCGGCTTGCGGTTCCTGATAGAGTTGATCGTCCAGAGCGACATCGCTTCCCGGACGCGCCAGCTTTTCTTCCAGCGCCAGCAGTTCAGCCAGGCGTGCAGGCGTGACGGCATAAAACGCCGGGGCATTGTTAGCAAATACGGCAACCACACCGCCTTCAGCTTTT  >  NC_000913/4601176‑4601443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑