Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	440,440	A→G	V221A (GTT→GCT)	ispA ←	geranyl diphosphate/farnesyl diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	440,440	0	A	G	100.0%	314.3 / NA	87	V221A (GTT→GCT)	ispA	geranyl diphosphate/farnesyl diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/46);  total (41/46)

GGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAAT  >  NC_000913/440313‑440573                                                                                                                                |                                                                                                                                      ggCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTg                                                                                                                                   >  1:473527/1‑132 (MQ=255) ggCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTg                                                                                                                                   <  2:473527/132‑1 (MQ=255) ggCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGaaggcaa                                                                                                                           >  1:1120226/1‑140 (MQ=255) ggCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGaaggcaa                                                                                                                           >  1:1311144/1‑140 (MQ=255)     ttcttcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGcc                                                                                                                       >  2:463608/1‑140 (MQ=255)     ttcttcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGcc                                                                                                                       >  2:347683/1‑140 (MQ=255)      tcttcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCg                                                                                                                      <  1:1341858/140‑1 (MQ=255)        ttcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGAt                                                                                                                    <  2:832861/140‑1 (MQ=255)        ttcCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGAt                                                                                                                    <  1:612534/140‑1 (MQ=255)           cGGGCTTGCTCAAGACCCAGACGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTTCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGct                                                                                                                 <  2:1298836/140‑1 (MQ=255)           cGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGct                                                                                                                 <  2:1120226/140‑1 (MQ=255)           cGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGct                                                                                                                 >  1:166396/1‑140 (MQ=255)           cGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGct                                                                                                                 <  1:1155012/140‑1 (MQ=255)             ggCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGAt                                                                                                                    >  1:807371/1‑135 (MQ=255)             ggCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGAt                                                                                                                    <  2:807371/135‑1 (MQ=255)                  gCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCa                                                                                                          >  1:458224/1‑140 (MQ=255)                  gCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCa                                                                                                          <  1:347683/140‑1 (MQ=255)                        gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGct                                                                                                                 <  1:964376/127‑1 (MQ=255)                        gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGct                                                                                                                 >  2:964376/1‑127 (MQ=255)                        gACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTg                                                                                                    >  2:515955/1‑140 (MQ=255)                          cccAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTc                                                                                                  <  1:515955/140‑1 (MQ=255)                          cccAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTc                                                                                                  <  2:459578/140‑1 (MQ=255)                           ccAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCg                                                                                                 <  2:1001470/140‑1 (MQ=255)                              gAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGt                                                                                              <  1:465480/140‑1 (MQ=255)                                aGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                                            <  1:300427/140‑1 (MQ=255)                                aGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                                            <  1:470360/140‑1 (MQ=255)                                aGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                                            <  2:880077/140‑1 (MQ=255)                                  tGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCg                                                                                          >  1:1289254/1‑140 (MQ=255)                                  tGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCg                                                                                          >  1:256370/1‑140 (MQ=255)                                  tGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCg                                                                                          >  1:1316458/1‑140 (MQ=255)                                  tGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCg                                                                                          >  1:139907/1‑140 (MQ=255)                                    cAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                                            <  2:533725/136‑1 (MQ=255)                                    cAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                                            >  1:533725/1‑136 (MQ=255)                                    cAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGc                                                                                        >  2:331225/1‑140 (MQ=255)                                         taggtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGc                                                                                   >  2:1170618/1‑140 (MQ=255)                                            gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                                            <  2:361780/128‑1 (MQ=255)                                            gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                                            >  1:361780/1‑128 (MQ=255)                                            gtaCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacg                                                                                >  2:686679/1‑140 (MQ=255)                                             taCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacga                                                                               <  2:1289254/140‑1 (MQ=255)                                              aCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgac                                                                              <  2:166396/140‑1 (MQ=255)                                              aCTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgac                                                                              <  2:247507/140‑1 (MQ=255)                                               cTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgacg                                                                             <  1:686679/140‑1 (MQ=255)                                               cTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgacg                                                                             <  2:193581/140‑1 (MQ=255)                                               cTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgacg                                                                             <  2:1316458/140‑1 (MQ=255)                                                 tttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTc                                                                           >  2:560002/1‑140 (MQ=255)                                                  ttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                                            >  1:64376/1‑122 (MQ=255)                                                  ttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTAc                                                                                            <  2:64376/122‑1 (MQ=255)                                                  ttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgac                                                                              >  1:112568/1‑136 (MQ=255)                                                  ttACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgac                                                                              <  2:112568/136‑1 (MQ=255)                                                     ccAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCttt                                                                       <  1:716198/140‑1 (MQ=255)                                                      cAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTa                                                                      >  1:346344/1‑140 (MQ=255)                                                          ttGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgac                                                                              <  2:613377/128‑1 (MQ=255)                                                          ttGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCacgac                                                                              >  1:613377/1‑128 (MQ=255)                                                          ttGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtctc                                                                  >  2:584695/1‑140 (MQ=255)                                                          ttGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTAtctc                                                                  >  1:1263534/1‑140 (MQ=255)                                                            gCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCg                                                                >  1:852469/1‑140 (MQ=255)                                                              tGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgc                                                              <  1:674518/140‑1 (MQ=255)                                                              tGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGgc                                                              <  2:256370/140‑1 (MQ=255)                                                                    gCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTaa                                                        >  1:123370/1‑140 (MQ=255)                                                                        ccTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCa                                                    <  1:331225/140‑1 (MQ=255)                                                                          tGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCAcc                                                  <  2:139907/140‑1 (MQ=255)                                                                          tGGCGTTTTCCCAACGTTGCAGTATATCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCg                                                                <  1:1082646/126‑1 (MQ=255)                                                                          tGGCGTTTTCCCAACGTTGCAGTATATCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCg                                                                >  2:1082646/1‑126 (MQ=255)                                                                                     cAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcgg                                       <  2:1311144/140‑1 (MQ=255)                                                                                     cAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAAcgg                                       <  1:560002/140‑1 (MQ=255)                                                                                            gCAGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGcgcg                                >  2:913867/1‑140 (MQ=255)                                                                                              aGTATCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGaa                              >  1:1238439/1‑140 (MQ=255)                                                                                                 aTCTCCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCa                           >  1:247759/1‑140 (MQ=255)                                                                                                  tctcCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCaa                          >  1:1408365/1‑140 (MQ=255)                                                                                                  tctcCCACCACATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCaa                          <  2:1238439/140‑1 (MQ=255)                                                                                                       caccacATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgcgc                     <  2:1358770/140‑1 (MQ=255)                                                                                                       caccacATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgcgc                     <  2:366301/140‑1 (MQ=255)                                                                                                       caccacATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgcgc                     >  2:1348639/1‑140 (MQ=255)                                                                                                       caccacATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATgcgc                     <  1:584695/140‑1 (MQ=255)                                                                                                        cccacATCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGcc                    >  2:313852/2‑140 (MQ=255)                                                                                                          cacaTCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCgg                  >  2:655243/1‑140 (MQ=255)                                                                                                            caTCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGtt                >  1:1301920/1‑140 (MQ=255)                                                                                                            caTCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGtt                >  2:490400/1‑140 (MQ=255)                                                                                                            caTCCAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGtt                >  2:234684/1‑140 (MQ=255)                                                                                                                cAGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTAt            <  2:346344/140‑1 (MQ=255)                                                                                                                cAGGACGTCATCCTGAGCCTGGAAGGTAAGGCCGATGCTCGCTGCAAACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTAt            <  2:1263534/140‑1 (MQ=255)                                                                                                                 aGGATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATg           <  1:125366/140‑1 (MQ=255)                                                                                                                   gATGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGAc         <  1:463608/140‑1 (MQ=255)                                                                                                                    aTGTCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACg        <  2:379081/140‑1 (MQ=255)                                                                                                                       tCATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATg     >  2:563604/1‑140 (MQ=255)                                                                                                                        cATCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGa    >  2:868820/1‑140 (MQ=255)                                                                                                                          tCCTGAGCCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAAt  <  1:563604/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                      GGCTTTCTTCCGGGCTTGCTCAAGACCCAGAAGTGCAGGGTAGGTACTTTTACCAAGTTGCTGGTCGGCACCCTGGCGTTTTCCCAACGTTGCAGTATCTCCCACCACATCCAGGATGTCATCCTGAACCTGGAAGGCAAGGCCGATGCTCTCTGCATACTTGTCGAGTACCGGCAGAGCACGACGTCCTTTATCTCCGGCGCTTAATGCACCAAGGCGAACGGCGGCGCGAATCAATGCGCCGGTTTTATGACGATGAAT  >  NC_000913/440313‑440573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑