Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	444,953	A→G	T91A (ACC→GCC)	yajQ →	nucleotide binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	444,953	0	A	G	100.0%	193.8 / NA	54	T91A (ACC→GCC)	yajQ	nucleotide binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/23);  total (31/23)

GTGTTGAGCGAGTCCGATTTCCAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAACCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACGGGCAAAT  >  NC_000913/444830‑445088                                                                                                                            |                                                                                                                                        gtgtTGAGCGAGTCCGATTTCTAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGc                                                                                                                         <  2:1300044/140‑1 (MQ=255) gtgtTGAGCGAGTCCGATTTCCAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGc                                                                                                                         <  1:1006414/140‑1 (MQ=255)  tgtTGAGCGAGTCCGATTTCCAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCg                                                                                                                        >  1:948437/1‑140 (MQ=255)   gtTGAGCGAGTCCGATTTCCAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGa                                                                                                                       <  1:316308/140‑1 (MQ=255)         cGAGTCCGATTTCCAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGa                                                                                                                 >  2:1027390/1‑140 (MQ=255)            gTCCGATTTCCAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAAc                                                                                                              >  2:603645/1‑140 (MQ=255)            gTCCGATTTCCAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAAc                                                                                                              <  1:1258869/140‑1 (MQ=255)            gTCCGATTTCCAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAAc                                                                                                              <  1:162056/140‑1 (MQ=255)             tCCGATTTCCAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACa                                                                                                             >  2:832816/1‑140 (MQ=255)                    tCCAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCAtt                                                                                                      <  2:1231565/140‑1 (MQ=255)                      cAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTga                                                                                                    <  1:1086650/140‑1 (MQ=255)                           cAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAgcg                                                                                               >  2:1082261/1‑140 (MQ=255)                              tCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGa                                                                                            >  1:214020/1‑140 (MQ=255)                                  ttGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCa                                                                                        >  1:1405541/1‑140 (MQ=255)                                   tGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCag                                                                                       >  1:20157/1‑140 (MQ=255)                                    gCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCaga                                                                                      >  1:160354/1‑140 (MQ=255)                                            ttCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATc                                                                              >  2:63928/1‑140 (MQ=255)                                             tCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCg                                                                             <  1:1082261/140‑1 (MQ=255)                                                gCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCa                                                                          >  1:933108/1‑140 (MQ=255)                                                 cGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCaa                                                                         <  1:1027390/140‑1 (MQ=255)                                                  gTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAg                                                                        >  1:145400/1‑140 (MQ=255)                                                  gTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAg                                                                        >  1:1068101/1‑140 (MQ=255)                                                  gTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAg                                                                        <  2:20157/140‑1 (MQ=255)                                                   tGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAga                                                                       <  1:303005/140‑1 (MQ=255)                                                   tGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAga                                                                       <  2:1421142/140‑1 (MQ=255)                                                   tGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAga                                                                       <  2:1068101/140‑1 (MQ=255)                                                   tGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAga                                                                       <  1:832816/140‑1 (MQ=255)                                                   tGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAga                                                                       <  2:475298/140‑1 (MQ=255)                                                   tGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAga                                                                       <  1:63928/140‑1 (MQ=255)                                                   tGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAga                                                                       <  1:41888/140‑1 (MQ=255)                                                             ctgAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGa                                                             >  1:1241506/1‑140 (MQ=255)                                                                       gCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTg                                                   >  2:1185462/1‑140 (MQ=255)                                                                                    ttCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGgcgc                                      <  2:223954/140‑1 (MQ=255)                                                                                    ttCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGgcgc                                      >  1:223954/1‑140 (MQ=255)                                                                                      cGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCaa                                    >  2:331445/1‑140 (MQ=255)                                                                                             tGTACCGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAgg                             <  2:145400/140‑1 (MQ=255)                                                                                                  cGGAGAATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGACCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGAt                        <  2:1241506/140‑1 (MQ=255)                                                                                                     agaATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGaa                     >  1:1059836/1‑140 (MQ=255)                                                                                                     agaATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGaa                     >  1:957192/1‑140 (MQ=255)                                                                                                      gaATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGaaa                    >  1:259736/1‑140 (MQ=255)                                                                                                      gaATATCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGaaa                    >  1:706457/1‑140 (MQ=255)                                                                                                         tatCGTTCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATcc                 >  2:219396/1‑140 (MQ=255)                                                                                                              ttCATAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTa            >  1:169869/1‑140 (MQ=255)                                                                                                                 aTAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACg         >  1:461457/1‑140 (MQ=255)                                                                                                                 aTAGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACg         >  1:651946/1‑140 (MQ=255)                                                                                                                   aGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACggg       >  2:145192/1‑140 (MQ=255)                                                                                                                   aGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACggg       >  1:864354/1‑140 (MQ=255)                                                                                                                   aGCGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACggg       >  2:613723/1‑140 (MQ=255)                                                                                                                     cGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACGGGCa     <  2:259736/140‑1 (MQ=255)                                                                                                                     cGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACGGGCa     <  2:706457/140‑1 (MQ=255)                                                                                                                     cGGTAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACGGGCa     >  2:1101836/1‑140 (MQ=255)                                                                                                                        tAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACGGGCAAAt  >  1:372894/1‑140 (MQ=255)                                                                                                                        tAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACGGGCAAAt  >  2:70546/1‑140 (MQ=255)                                                                                                                        tAAAGCCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACGGGCAAAt  <  1:145192/140‑1 (MQ=255)                                                                                                                            |                                                                                                                                        GTGTTGAGCGAGTCCGATTTCCAGGTCAATCAGTTGCTGGATATTCTGCGTGCCAAGCTGCTGAAGCGCGGCATTGAAGGCAGTTCGCTGGATGTACCGGAGAATATCGTTCATAGCGGTAAAACCTGGTTTGTGGAAGCGAAACTGAAACAGGGCATTGAGAGCGCGACCCAGAAGAAAATCGTCAAGATGATCAAAGACAGCAAACTGAAAGTGCAGGCGCAAATTCAGGGCGATGAAATCCGCGTAACGGGCAAAT  >  NC_000913/444830‑445088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑