Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	494,430	A→G	Q9R (CAG→CGG)	recR →	DNA repair protein RecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	494,430	0	A	G	100.0%	181.0 / NA	51	Q9R (CAG→CGG)	recR	DNA repair protein RecR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/26);  total (25/26)

GGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTC  >  NC_000913/494294‑494559                                                                                                                                         |                                                                                                                                  ggCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCt                                                                                                                                <  1:733262/140‑1 (MQ=255)         caTTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAg                                                                                                                        <  1:1158804/140‑1 (MQ=255)                 gacgCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTgcgc                                                                                                                <  1:511261/140‑1 (MQ=255)                   cgCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTg                                                                                                              >  1:565229/1‑140 (MQ=255)                     cagcaCGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTc                                                                                                            >  2:215636/1‑140 (MQ=255)                             cgtATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGc                                                                                                    >  2:282799/1‑140 (MQ=255)                             cgtATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGc                                                                                                    >  2:207510/1‑140 (MQ=255)                                    aagaaACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGcc                                                                                             >  2:1180418/1‑140 (MQ=255)                                         aCGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCg                                                                                                   <  2:286804/129‑1 (MQ=255)                                         aCGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCg                                                                                                   >  1:286804/1‑129 (MQ=255)                                         aCGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAg                                                                                        >  2:48546/1‑140 (MQ=255)                                         aCGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAg                                                                                        >  2:1205827/1‑140 (MQ=255)                                         aCGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAg                                                                                        >  2:758974/1‑140 (MQ=255)                                         aCGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTAAGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAg                                                                                        >  2:470221/1‑140 (MQ=255)                                         aCGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGCTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAg                                                                                        >  2:1385341/1‑140 (MQ=255)                                          cGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGt                                                                                       <  2:1170922/140‑1 (MQ=255)                                            cAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCg                                                                                     >  1:123542/1‑140 (MQ=255)                                              gaaagaaaAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCCCTGCGCTGTCTGCCCGGCGTTGGCCCGAAGTCGgc                                                                                   <  1:282799/140‑1 (MQ=255)                                              gaaagaaaAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTAAGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgc                                                                                   <  1:470221/140‑1 (MQ=255)                                               aaagaaaAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgcg                                                                                  <  1:590128/140‑1 (MQ=255)                                               aaagaaaAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgcg                                                                                  <  1:1205827/140‑1 (MQ=255)                                               aaagaaaAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGgcg                                                                                  <  1:205206/140‑1 (MQ=255)                                                 agaaaAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCa                                                                                <  2:123542/140‑1 (MQ=255)                                                 agaaaAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCa                                                                                <  2:565229/140‑1 (MQ=255)                                                       aaTGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTAt                                                                          <  1:48546/140‑1 (MQ=255)                                                             ctctGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGtt                                                                    <  2:454211/140‑1 (MQ=255)                                                                 gTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGCTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                                <  1:1385341/140‑1 (MQ=255)                                                                 gTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGAAGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                                <  2:600941/140‑1 (MQ=255)                                                                     cctccGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACgctgc                                                            >  1:693084/1‑140 (MQ=255)                                                                         cGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCa                                                        >  1:268439/1‑140 (MQ=255)                                                                          ggAATGTAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAg                                                       >  2:1212682/1‑140 (MQ=255)                                                                          ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAg                                                       >  1:927353/1‑140 (MQ=255)                                                                          ggAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAg                                                       >  1:762135/1‑140 (MQ=255)                                                                            aaTGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAgcg                                                     >  1:441607/1‑140 (MQ=255)                                                                              tGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGcgcg                                                   >  2:235061/1‑140 (MQ=255)                                                                                cAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGAt                                                 >  1:382782/1‑140 (MQ=255)                                                                                cAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGAt                                                 >  1:360455/1‑140 (MQ=255)                                                                                 aGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                                <  2:337826/124‑1 (MQ=255)                                                                                 aGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACg                                                                >  1:337826/1‑124 (MQ=255)                                                                                     gccgccTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAg                                            >  2:688042/1‑140 (MQ=255)                                                                                        gccTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAgcgg                                         <  2:762135/140‑1 (MQ=255)                                                                                        gccTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAgcgg                                         <  1:942205/140‑1 (MQ=255)                                                                                         ccTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAgcggc                                        <  2:1405383/140‑1 (MQ=255)                                                                                              cTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGAt                                   <  2:382782/140‑1 (MQ=255)                                                                                              cTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGAt                                   <  1:1212682/140‑1 (MQ=255)                                                                                                    gATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCt                             <  2:441607/140‑1 (MQ=255)                                                                                                        ccGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGgcg                         >  2:1116227/1‑140 (MQ=255)                                                                                                        ccGTTCTGATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGgcg                         >  1:757040/1‑140 (MQ=255)                                                                                                               gATGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGgcgc                  <  1:1180418/140‑1 (MQ=255)                                                                                                                aTGCAAACCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCt                 <  1:235061/140‑1 (MQ=255)                                                                                                                     aaCCAGCCCGCTGTTAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCCCAccc            >  2:1315010/1‑140 (MQ=255)                                                                                                                               acagtAACACGGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTc  <  1:1399294/136‑1 (MQ=255)                                                                                                                                         |                                                                                                                                  GGCTGCAGCATTCAACGACGCAGCACGTCGTATTGAAGAAACGCAGAAAGAAAAAATGGCCTCTGTATCCTCCGGAATGCAGCTGCCGCCTGGCTTTAAGATGCCGTTCTGATGCAAACCAGCCCGCTGTTAACACAGCTTATGGAAGCACTGCGCTGTCTGCCGGGCGTTGGCCCGAAGTCGGCGCAGCGTATGGCGTTCACGCTGCTTCAGCGCGATCGTAGCGGCGGGATGCGTCTGGCGCAGGCGCTCACCCGGGCGATGTC  >  NC_000913/494294‑494559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑