Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	535,327	T→C	V471A (GTC→GCC)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	535,327	0	T	C	100.0%	216.4 / NA	61	V471A (GTC→GCC)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/39);  total (22/39)

TCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGG  >  NC_000913/535190‑535461                                                                                                                                          |                                                                                                                                       tCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCca                                                                                                                                      >  1:987964/1‑140 (MQ=255)  ccGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCcaa                                                                                                                                     <  2:613801/140‑1 (MQ=255)    ggCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCcaaca                                                                                                                                   <  1:400424/140‑1 (MQ=255)       tGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACg                                                                                                                                <  2:1326075/140‑1 (MQ=255)        gcgcTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGc                                                                                                                               <  1:973655/140‑1 (MQ=255)          gcTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCtt                                                                                                                             >  1:932933/1‑140 (MQ=255)               gggTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTg                                                                                                                        <  2:978307/140‑1 (MQ=255)                 gTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTggg                                                                                                                      <  2:887840/140‑1 (MQ=255)                       gCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGAt                                                                                                                <  1:1213985/140‑1 (MQ=255)                       gCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGAt                                                                                                                <  1:1321425/140‑1 (MQ=255)                              aTCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCgtcag                                                                                                         >  1:1267223/1‑140 (MQ=255)                                     aCGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAAc                                                                                                  <  2:1267223/140‑1 (MQ=255)                                        cAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAAcgcg                                                                                               >  1:868016/1‑140 (MQ=255)                                              ggtggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                                         <  2:56066/140‑1 (MQ=255)                                              ggtggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                                         <  2:1225545/140‑1 (MQ=255)                                              ggtggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                                         <  2:530730/140‑1 (MQ=255)                                              ggtggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                                         <  1:989133/140‑1 (MQ=255)                                              ggtggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTg                                                                                         <  2:133980/140‑1 (MQ=255)                                                tggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGAc                                                                                       <  1:489926/140‑1 (MQ=255)                                                tggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGAc                                                                                       <  2:1049418/140‑1 (MQ=255)                                                 ggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGTTGATTCGTCAGTCACAACGCGCTTTTg                                                                                         >  2:1214787/1‑137 (MQ=255)                                                 ggCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGTTGATTCGTCAGTCACAACGCGCTTTTg                                                                                         <  1:1214787/137‑1 (MQ=255)                                                   cGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATg                                                                                    >  1:1099800/1‑140 (MQ=255)                                                             cGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCg                                                                          <  2:712676/140‑1 (MQ=255)                                                                   tGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGATTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAAc                                                                    <  2:987964/140‑1 (MQ=255)                                                                      cTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCg                                                                 <  1:733688/140‑1 (MQ=255)                                                                      cTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCg                                                                 <  2:1061396/140‑1 (MQ=255)                                                                           aGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTc                                                            >  2:693738/1‑140 (MQ=255)                                                                           aGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTc                                                            <  1:693738/140‑1 (MQ=255)                                                                               ccTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCgaga                                                        >  1:653604/1‑140 (MQ=255)                                                                                cTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCgaga                                                        <  1:241050/139‑1 (MQ=255)                                                                                cTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCgaga                                                        >  2:241050/1‑139 (MQ=255)                                                                                  gATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAata                                                     >  1:471062/1‑140 (MQ=255)                                                                                   aTTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAatat                                                    <  2:1099800/140‑1 (MQ=255)                                                                                        agagTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACt                                               <  2:653604/140‑1 (MQ=255)                                                                                            ttAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCag                                           >  2:158401/1‑140 (MQ=255)                                                                                                cTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtgaa                                       <  2:1347994/140‑1 (MQ=255)                                                                                                cTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCagtgaa                                       <  2:87289/140‑1 (MQ=255)                                                                                                     ggCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAgtgaagtgaa                                  >  1:31061/1‑140 (MQ=255)                                                                                                      gcgcgcAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAAt                                 >  1:203167/1‑140 (MQ=255)                                                                                                           cAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTa                            <  2:747763/140‑1 (MQ=255)                                                                                                           cAGTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTa                            <  2:471062/140‑1 (MQ=255)                                                                                                             gTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACg                          >  1:145570/1‑140 (MQ=255)                                                                                                             gTTCAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACg                          >  1:688931/1‑140 (MQ=255)                                                                                                                cAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGgtg                       <  2:932933/140‑1 (MQ=255)                                                                                                                cAACATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACCGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGgtg                       >  2:793372/1‑140 (MQ=255)                                                                                                                  aCATTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGtt                     >  2:997528/1‑140 (MQ=255)                                                                                                                    aTTCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGa                   <  2:203167/140‑1 (MQ=255)                                                                                                                     ttCCGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGAc                  <  1:158401/140‑1 (MQ=255)                                                                                                                        cGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCt                             >  2:704095/1‑126 (MQ=255)                                                                                                                        cGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCt                             <  1:704095/126‑1 (MQ=255)                                                                                                                        cGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGAc                  >  2:1413436/1‑137 (MQ=255)                                                                                                                        cGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGAc                  <  1:1413436/137‑1 (MQ=255)                                                                                                                        cGTACATCCATGTGCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCAc               >  2:249311/1‑140 (MQ=255)                                                                                                                                  tgtgCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAg     >  1:1328615/1‑140 (MQ=255)                                                                                                                                    tgCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGc    <  1:503199/139‑1 (MQ=255)                                                                                                                                    tgCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGc    >  2:503199/1‑139 (MQ=255)                                                                                                                                     gCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCgg  <  1:249311/140‑1 (MQ=255)                                                                                                                                     gCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCgg  <  2:31061/140‑1 (MQ=255)                                                                                                                                     gCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCgg  <  2:1328615/140‑1 (MQ=255)                                                                                                                                     gCTGGCCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACCGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCgg  <  1:793372/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TCCGGCTGCGCTAGGGGTTTGTGCCGCTGATCCGAAACGCAATGTGGTGGCGATTTCTGGCGACTTTGACTTCCAGTTCCTGATTGAAGAGTTAGCTGTTGGCGCGCAGTTCAACATTCCGTACATCCATGTGCTGGTCAACAACGCTTATCTGGGGCTGATTCGTCAGTCACAACGCGCTTTTGACATGGACTACTGCGTGCAACTCGCTTTCGAGAATATCAACTCCAGTGAAGTGAATGGCTACGGTGTTGACCACGTAAAAGTAGCGG  >  NC_000913/535190‑535461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑