Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	605,892	T→C	intergenic (‑6/+59)	ybdF ← / ← ybdJ	PF04237 family protein YbdF/DUF1158 domain‑containing protein YbdJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	605,892	0	T	C	100.0%	184.7 / NA	52	intergenic (‑6/+59)	ybdF/ybdJ	PF04237 family protein YbdF/DUF1158 domain‑containing protein YbdJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

TAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGTTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACC  >  NC_000913/605766‑606027                                                                                                                               |                                                                                                                                        tAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGaa                                                                                                                            <  1:290008/140‑1 (MQ=255) tAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGaa                                                                                                                            <  2:835158/140‑1 (MQ=255)    aaaTCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGga                                                                                                                         >  1:555598/1‑140 (MQ=255)      aTCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGgaga                                                                                                                       <  2:1145640/140‑1 (MQ=255)       tCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGaa                                                                                                                      >  2:1195186/1‑140 (MQ=255)        cTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAAt                                                                                                                     <  1:141411/140‑1 (MQ=255)                  aTTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTa                                                                                                           <  2:43697/140‑1 (MQ=255)                    tttAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATg                                                                                                         <  2:875883/140‑1 (MQ=255)                         aaaCATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCcgcg                                                                                                    <  1:1195186/140‑1 (MQ=255)                             aTCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGt                                                                                                >  1:1412907/1‑140 (MQ=255)                                      cGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATa                                                                                       >  1:223934/1‑140 (MQ=255)                                       gggCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGAt                                                                                        >  2:473228/1‑138 (MQ=255)                                       gggCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGAt                                                                                        <  1:473228/138‑1 (MQ=255)                                                ggCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGttt                                                                             >  1:1286581/1‑140 (MQ=255)                                                   cAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAAt                                                                          <  2:1286581/140‑1 (MQ=255)                                                       aaaGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGc                                                                      <  1:1173426/140‑1 (MQ=255)                                                          gCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAg                                                                   >  1:1033251/1‑140 (MQ=255)                                                           cTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGc                                                                  >  1:1237442/1‑140 (MQ=255)                                                                    gggTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAg                                                         <  2:1412907/140‑1 (MQ=255)                                                                      gTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCg                                                       >  1:782243/1‑140 (MQ=255)                                                                               gggCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGcc                                                  <  1:468764/136‑1 (MQ=255)                                                                               gggCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGcc                                                  >  2:468764/1‑136 (MQ=255)                                                                               gggCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGAt                                              >  2:540481/1‑140 (MQ=255)                                                                               gggCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGAt                                              >  1:624911/1‑140 (MQ=255)                                                                               gggCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGAt                                              >  2:1155705/1‑140 (MQ=255)                                                                               gggCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGAt                                              >  1:1226234/1‑140 (MQ=255)                                                                               gggCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGAt                                              >  1:925305/1‑140 (MQ=255)                                                                               gggCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGAt                                              >  2:1139578/1‑140 (MQ=255)                                                                                    aGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAgc                                         >  1:643468/1‑140 (MQ=255)                                                                                    aGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAgc                                         >  2:1241986/1‑140 (MQ=255)                                                                                     ggCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAgcg                                        >  2:934505/1‑140 (MQ=255)                                                                                     ggCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAgcg                                        >  1:652240/1‑140 (MQ=255)                                                                                     ggCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCC‑‑‑‑GCGAGAACCAGCGTCGCCAGATAAAGCGCAGa                                    <  2:223934/140‑1 (MQ=255)                                                                                       cGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCa                                      <  2:652240/140‑1 (MQ=255)                                                                                            cGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACa                                 <  2:925305/140‑1 (MQ=255)                                                                                            cGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACa                                 <  1:1139578/140‑1 (MQ=255)                                                                                                gTTTCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAAt                             <  2:1178419/140‑1 (MQ=255)                                                                                                  ttCGTGCAGTGATTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATAc                           >  1:1250339/1‑140 (MQ=255)                                                                                                             aTTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGcc                >  1:661887/1‑140 (MQ=255)                                                                                                             aTTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGcc                <  1:540481/140‑1 (MQ=255)                                                                                                             aTTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGcc                >  1:188507/1‑140 (MQ=255)                                                                                                             aTTGCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGcc                >  1:1427706/1‑140 (MQ=255)                                                                                                                gCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAg             >  1:586640/1‑140 (MQ=255)                                                                                                                gCTTATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAg             <  2:782243/140‑1 (MQ=255)                                                                                                                  ttATCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCa           <  2:1226234/140‑1 (MQ=255)                                                                                                                    aTCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACt         <  1:1155705/140‑1 (MQ=255)                                                                                                                     tCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACt         >  1:728251/1‑139 (MQ=255)                                                                                                                     tCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACt         <  2:728251/139‑1 (MQ=255)                                                                                                                     tCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACt         <  2:292732/139‑1 (MQ=255)                                                                                                                     tCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACt         >  1:292732/1‑139 (MQ=255)                                                                                                                     tCCATAAACGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTa        >  1:1091702/1‑140 (MQ=255)                                                                                                                           aaCGCTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAAcc  >  2:1172408/1‑140 (MQ=255)                                                                                                                               |                                                                                                                                        TAAAAATCTTGCCGCCAATTTTAAAAACATCGAACTCCGGGCCAAAAGGCCAGCAAAGCTCGACAAAGGGTAACTCAAGGGCCAGGCGTTTCGCCGTTTCGTGCAGTGATTGCTTATCCATAAACGTTCCTTTAGGCGAAGGAGAATAAGCAAAGTATGCCGCGAAGTACGGCGATAATCGACGTTTAATCCGCCAGCGAGAACCAGCGTCGCCAGATAAAGCGCAGAACAAAATACTCAATAGCGCCCAGCACTAAAAACC  >  NC_000913/605766‑606027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑