Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	699,394	C→G	intergenic (‑217/+180)	asnB ← / ← umpH	asparagine synthetase B/UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	699,394	0	C	G	100.0%	185.8 / NA	52	intergenic (‑217/+180)	asnB/umpH	asparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (26/26);  total (26/26)

GGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGG  >  NC_000913/699269‑699529                                                                                                                              |                                                                                                                                        ggTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACaa                                                                                                                           >  1:940955/1‑140 (MQ=255)         aaTAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGAt                                                                                                                   <  1:145654/140‑1 (MQ=255)          aTAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGAtt                                                                                                                  <  2:86433/140‑1 (MQ=255)             aaaaaCGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGt                                                                                                               >  2:272061/1‑140 (MQ=255)                           gATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATgcg                                                                                                 <  2:803146/140‑1 (MQ=255)                            aTTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATgcgg                                                                                                >  1:403607/1‑140 (MQ=255)                             ttGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATgcggc                                                                                               <  1:923407/140‑1 (MQ=255)                                tCGATTGGTGAAAAAAGGTCTGGTGTGAGGATGTATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTg                                                                                            <  1:778241/140‑1 (MQ=255)                                tCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTg                                                                                            <  2:122097/140‑1 (MQ=255)                                 cGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGa                                                                                           <  2:1178707/140‑1 (MQ=255)                                   aTTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAAc                                                                                         >  1:1144072/1‑140 (MQ=255)                                                   cTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTAc                                                                         <  2:348965/140‑1 (MQ=255)                                                           aGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGTGGCGTGAACGCCTTATCCGGCCTACAAAAGCAt                                                                 >  1:581224/1‑140 (MQ=255)                                                           aGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCAt                                                                 >  2:107779/1‑140 (MQ=255)                                                           aGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGCTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCAt                                                                 >  2:418663/1‑140 (MQ=255)                                                            ggATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATg                                                                <  2:1144072/140‑1 (MQ=255)                                                                atatTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGaaaa                                                            >  2:1402387/1‑140 (MQ=255)                                                                atatTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGaaaa                                                            >  1:1030711/1‑140 (MQ=255)                                                                atatTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGaaaa                                                            >  1:1204867/1‑140 (MQ=255)                                                                  atttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAAtt                                                          <  2:243241/140‑1 (MQ=255)                                                                  atttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGCTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAAtt                                                          <  1:418663/140‑1 (MQ=255)                                                                     tattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCaa                                                       >  1:991412/1‑140 (MQ=255)                                                                      attGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaa                                                                       <  2:779133/123‑1 (MQ=255)                                                                      attGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaa                                                                       >  1:779133/1‑123 (MQ=255)                                                                       ttGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAata                                                     >  1:391838/1‑140 (MQ=255)                                                                        tgatgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAatat                                                    >  2:621184/1‑140 (MQ=255)                                                                        tgatgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAatat                                                    <  2:758455/140‑1 (MQ=255)                                                                        tgatgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAatat                                                    >  1:187886/1‑140 (MQ=255)                                                                          atgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAatatat                                                  >  1:36027/1‑140 (MQ=255)                                                                            gaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCaa                                                       >  1:387131/1‑133 (MQ=255)                                                                            gaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCaa                                                       <  2:387131/133‑1 (MQ=255)                                                                             aaTCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGc                                               <  1:420307/140‑1 (MQ=255)                                                                             aaTCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGc                                               >  1:108793/1‑140 (MQ=255)                                                                              aTCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCa                                              >  1:1324170/1‑140 (MQ=255)                                                                                  aTAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAg                                          <  2:187886/140‑1 (MQ=255)                                                                                  aTAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAg                                          <  2:36027/140‑1 (MQ=255)                                                                                       ttttAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCg                                     <  2:1204867/140‑1 (MQ=255)                                                                                       ttttAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCg                                     <  2:319251/140‑1 (MQ=255)                                                                                       ttttAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCg                                     <  1:107779/140‑1 (MQ=255)                                                                                       ttttAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCg                                     <  2:403607/140‑1 (MQ=255)                                                                                           aGCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAgg                                 <  1:1402387/140‑1 (MQ=255)                                                                                            gCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGc                                <  2:1151788/140‑1 (MQ=255)                                                                                            gCGGGTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGc                                <  1:621184/140‑1 (MQ=255)                                                                                                gTTTTATTGAATGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGa                            >  1:1008764/1‑140 (MQ=255)                                                                                                           tGTTTATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAgcg                 >  2:1420896/1‑140 (MQ=255)                                                                                                             tttATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCa               <  2:1030711/140‑1 (MQ=255)                                                                                                              ttATATTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCAt              >  1:1402798/1‑140 (MQ=255)                                                                                                               tataTTTTACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATc             >  1:56017/1‑140 (MQ=255)                                                                                                                     ttACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAg       >  1:561367/1‑140 (MQ=255)                                                                                                                      tACGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGt      >  2:635626/1‑140 (MQ=255)                                                                                                                       aCGGGGGGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGtt     >  2:911244/1‑140 (MQ=255)                                                                                                                          ggggGCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTgg  <  2:560935/140‑1 (MQ=255)                                                                                                                              |                                                                                                                                        GGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGG  >  NC_000913/699269‑699529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑