Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	132,880	T→C	R422R (CGT→CGC)	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	132,880	0	T	C	100.0%	201.8 / NA	57	R422R (CGT→CGC)	acnB	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/33);  total (24/33)

GAGCGATCGCGGCTTCTCGCTGGCGCAAAAAATGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATT  >  NC_000913/132745‑133006                                                                                                                                        |                                                                                                                               gagCGATCGCGGCTTCTCGCTGGCGCAAAAAATGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGcgatg                                                                                                                            <  2:534389/140‑1 (MQ=255) gagCGATCGCGGCTTCTCGCTGGCGCAAAAAATGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGcgatg                                                                                                                            <  2:598333/140‑1 (MQ=255)       tCGCGGCTTCTCGCTGGCGCAAAAAATGGTAGGCCGTGCCTGTGGGGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGa                                                                                                                      <  1:425758/140‑1 (MQ=255)           ggCTTCTCGCTGGCGCAAAAAATGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGa                                                                                                                  <  2:255195/140‑1 (MQ=255)                ctcGCTGGCGCAAAAAATGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTgg                                                                                                             <  2:712399/140‑1 (MQ=255)                ctcGCTGGCGCAAAAAATGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTgg                                                                                                             <  1:767026/140‑1 (MQ=255)                ctcGCTGGCGCAAAAAATGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTgg                                                                                                             <  1:1339504/140‑1 (MQ=255)                ctcGCTGGCGCAAAAAATGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTgg                                                                                                             <  1:1138332/140‑1 (MQ=255)                          cAAAAAATGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTggg                                                                                                   >  2:484816/1‑140 (MQ=255)                             aaaaTGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCtt                                                                                                <  1:70757/140‑1 (MQ=255)                                 tGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCg                                                                                            <  1:38896/140‑1 (MQ=255)                                  ggTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCgg                                                                                           <  1:170558/140‑1 (MQ=255)                                        ccGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGAcc                                                                                     <  1:71417/140‑1 (MQ=255)                                         cGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCt                                                                                    >  2:668995/1‑140 (MQ=255)                                           tGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCtt                                                                                                >  1:301781/1‑126 (MQ=255)                                           tGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCtt                                                                                                <  2:301781/126‑1 (MQ=255)                                            gCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGt                                                                                 >  2:376330/1‑140 (MQ=255)                                                 tgGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCt                                                                                         <  2:146086/127‑1 (MQ=255)                                                 tgGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCt                                                                                         >  1:146086/1‑127 (MQ=255)                                                     gTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCT‑GGCTTCTCGGCTGACCTGGTGATGCAGTCt                                                                       <  2:1086225/140‑1 (MQ=255)                                                      tGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCt                                                                       >  2:582518/1‑140 (MQ=255)                                                         aaGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTc                                                                    <  2:476491/140‑1 (MQ=255)                                                             cATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGcc                                                                >  2:1286314/1‑140 (MQ=255)                                                             cATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCCGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGcc                                                                <  2:578459/140‑1 (MQ=255)                                                                          gcgTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTa                                                   >  2:1365482/1‑140 (MQ=255)                                                                              aCTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCg                                               <  1:1365482/140‑1 (MQ=255)                                                                                  tgAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGc                                           >  1:482926/1‑140 (MQ=255)                                                                                  tgAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGc                                           >  1:21720/1‑140 (MQ=255)                                                                                    aaCCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATcc                                                >  2:303804/1‑133 (MQ=255)                                                                                    aaCCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATcc                                                <  1:303804/133‑1 (MQ=255)                                                                                    aaCCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCa                                         >  2:1335326/1‑140 (MQ=255)                                                                                       cGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAGGACCTGGCGTGCCTGGGATTCTCGGCTAACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGtt                                      >  2:1276475/1‑140 (MQ=255)                                                                                            aTGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGt                                 >  1:1188232/1‑140 (MQ=255)                                                                                                      tAGGTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGcac                       >  1:787987/1‑140 (MQ=255)                                                                                                         gTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACg                                  >  2:936363/1‑126 (MQ=255)                                                                                                         gTTCCCAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACg                                  <  1:936363/126‑1 (MQ=255)                                                                                                             ccAGGACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGc                >  1:1014794/1‑140 (MQ=255)                                                                                                                ggACACCACCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGc             <  1:484816/140‑1 (MQ=255)                                                                                                                  acacCGCCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACg                          >  2:895891/1‑125 (MQ=255)                                                                                                                  acacCGCCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACg                          <  1:895891/125‑1 (MQ=255)                                                                                                                   caccgcCGGCCCGATGACCCGCGATGAACTGAAAGACCGGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCgg          <  2:720472/140‑1 (MQ=255)                                                                                                                   caccacCGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCgg          <  1:737568/140‑1 (MQ=255)                                                                                                                   caccacCGGCCCGATGACCCGCGATGAACTG‑AAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGa         >  2:1235925/1‑140 (MQ=255)                                                                                                                    accaccGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGa         <  2:1139110/140‑1 (MQ=255)                                                                                                                    accaccGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGa         <  2:21720/140‑1 (MQ=255)                                                                                                                    accaccGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGa         <  2:155371/140‑1 (MQ=255)                                                                                                                     ccaccGGCCCGATGACCCGCGATGAACTG‑AAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACt       <  1:1235925/140‑1 (MQ=255)                                                                                                                      caccGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACcaca                   >  1:50746/1‑128 (MQ=255)                                                                                                                      caccGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACcaca                   <  2:50746/128‑1 (MQ=255)                                                                                                                      caccGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACt       >  2:1060281/1‑140 (MQ=255)                                                                                                                      caccGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACt       <  1:582518/140‑1 (MQ=255)                                                                                                                      caccGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACt       <  1:189752/140‑1 (MQ=255)                                                                                                                      caccGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACt       <  1:1286314/140‑1 (MQ=255)                                                                                                                      caccGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACt       <  2:787987/140‑1 (MQ=255)                                                                                                                       accGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACtt      >  2:1416985/1‑140 (MQ=255)                                                                                                                        ccGGCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTc     <  1:376330/140‑1 (MQ=255)                                                                                                                           gCCCGATGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCAtt  >  2:885855/1‑140 (MQ=255)                                                                                                                                aTGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACt       >  1:1113606/1‑130 (MQ=255)                                                                                                                                aTGACCCGCGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACt       <  2:1113606/130‑1 (MQ=255)                                                                                                                                        |                                                                                                                               GAGCGATCGCGGCTTCTCGCTGGCGCAAAAAATGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATT  >  NC_000913/132745‑133006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑