Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	948,785	T→C	T262A (ACA→GCA)	ycaN ←	putative LysR‑type transcriptional regulator YcaN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	948,785	0	T	C	100.0%	146.5 / NA	42	T262A (ACA→GCA)	ycaN	putative LysR‑type transcriptional regulator YcaN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/31);  total (11/31)

GTAAATAATTATTGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGTGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATT  >  NC_000913/948652‑948920                                                                                                                                      |                                                                                                                                        gTAAATAATTATTGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCa                                                                                                                                   <  2:523447/140‑1 (MQ=255)  tAAATAATTATTGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAg                                                                                                                                  <  2:1001782/140‑1 (MQ=255)  tAAATAATTATTGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCATTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAg                                                                                                                                  <  2:167529/140‑1 (MQ=255)    aataatTATTGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGAGAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTc                                                                                                                                <  2:404103/140‑1 (MQ=255)      cagttattGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCtt                                                                                                                              <  1:650448/137‑1 (MQ=255)            ttGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTTGATAATAGATCTGGAAGCCCGGACGCTCTGCGCTCCAGTCTTTCAGTa                                                                                                                        >  1:691952/1‑140 (MQ=255)            ttGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTa                                                                                                                        <  1:1083471/140‑1 (MQ=255)            ttGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTa                                                                                                                        <  2:307440/140‑1 (MQ=255)            ttGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTa                                                                                                                        >  1:743163/1‑140 (MQ=255)            ttGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTa                                                                                                                        >  1:430979/1‑140 (MQ=255)            ttGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTa                                                                                                                        >  2:951238/1‑140 (MQ=255)                 gTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTTGATAATAAAACTGGAAGCCTGGACGCCCTGCGCTCCCGTCTTCCAGTAcacaa                                                                                                                   >  2:701139/1‑139 (MQ=255)                 gTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTc                                                                                                                                <  2:1014803/127‑1 (MQ=255)                 gTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTc                                                                                                                                >  1:1014803/1‑127 (MQ=255)                 gTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACAt                                                                                                                   >  2:284386/1‑140 (MQ=255)                 gTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACAt                                                                                                                   <  1:1374192/140‑1 (MQ=255)                 gTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAAAAAATCTGGAAGCCTGGACGCTCTGCGCGCCCGTCTTCCCGTACACAt                                                                                                                   >  2:1083471/1‑140 (MQ=255)                    ggTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGGTAATAAATCTGGAAGCCTGGACGCTCTTCGCTCCAGTCTTCCAGTACACAtctt                                                                                                                >  2:650448/1‑137 (MQ=255)                           cTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGAc                                                                                                         <  1:248868/140‑1 (MQ=255)                           cTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGAc                                                                                                         <  1:284386/140‑1 (MQ=255)                           cTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGAc                                                                                                         <  2:588762/140‑1 (MQ=255)                              aCTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAg                                                                                                      <  2:768258/140‑1 (MQ=255)                                       gATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGt                                                                                             <  1:130203/140‑1 (MQ=255)                                         tCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTAc                                                                                           <  1:390554/140‑1 (MQ=255)                                          cTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACt                                                                                          >  1:821243/1‑140 (MQ=255)                                              cccGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTTGATAATAAATCTGGAAGCCCGGACGCTCTGCGCTCCCGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGGACTCCtt                                                                                      >  2:638883/1‑140 (MQ=255)                                                cGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGa                                                                                    <  1:701139/140‑1 (MQ=255)                                                   ttttACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCt                                                                                 <  2:1029587/140‑1 (MQ=255)                                                          tAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATa                                                                          <  1:75213/140‑1 (MQ=255)                                                          tAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATa                                                                          <  1:1372114/140‑1 (MQ=255)                                                            aTCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATaga                                                                        >  2:502194/1‑140 (MQ=255)                                                            aTCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATaga                                                                        >  1:152503/1‑140 (MQ=255)                                                             tCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTTGATAATAAATCTGGAAGCCTGGACGCCCTGCGCTCCCGTCTTCCAGTACACATTCTAAAAGACCAGTATCAAGGGACTCCTTTATCTGTTCATagag                                                                       >  2:1372114/1‑140 (MQ=255)                                                             tCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCt                                                                                 <  2:534354/130‑1 (MQ=255)                                                             tCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCt                                                                                 >  1:534354/1‑130 (MQ=255)                                                                 gaAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGa                                                                   <  2:152503/140‑1 (MQ=255)                                                                  aaaTGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGAt                                                                  >  1:1275576/1‑140 (MQ=255)                                                                       cgcgcAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCg                                                             >  2:960304/1‑140 (MQ=255)                                                                       cgcgcAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAAGGACTCCTTGATCTGTTCATAGAGCAGATAaccc                                                             >  2:844389/1‑139 (MQ=255)                                                                           cAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATAc                                                         <  1:951238/140‑1 (MQ=255)                                                                                  cAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCAcc                                                  <  1:960304/140‑1 (MQ=255)                                                                                          ataCTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCaaaa                                          >  1:894362/1‑140 (MQ=255)                                                                                            aCTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACg                                        <  1:371528/140‑1 (MQ=255)                                                                                               ggCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGcc                                     >  2:952602/1‑140 (MQ=255)                                                                                                 cGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTc                                   <  2:691952/140‑1 (MQ=255)                                                                                                     tttgATAAAAGATCTGGAAGCCTGGACGCCCTGCGCTCCCGTCTTCCAGTACACATTCTAAACGACCAGTATCAAAGGACTCCTTGATCTTTTTATAGAGCAGATACCCGATAACAGCACCCATCAAAACAGCCTCCAGt                               >  2:1104357/4‑140 (MQ=255)                                                                                                      tGGATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGtt                              <  1:502194/140‑1 (MQ=255)                                                                                                       ggATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTc                             <  2:821243/140‑1 (MQ=255)                                                                                                       ggATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTc                             <  1:952602/140‑1 (MQ=255)                                                                                                       ggATAATAGATCTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTc                             <  1:1104357/140‑1 (MQ=255)                                                                                                                  cTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAAcat                  >  1:444971/1‑140 (MQ=255)                                                                                                                  cTGGAAGCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAAcat                  >  1:1362579/1‑140 (MQ=255)                                                                                                                        gCCTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCa            <  2:444971/140‑1 (MQ=255)                                                                                                                          cTGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGa          >  1:89224/1‑140 (MQ=255)                                                                                                                           tGGACGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGaa         >  2:1231315/1‑140 (MQ=255)                                                                                                                              aCGCTCTGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGa      <  2:89224/140‑1 (MQ=255)                                                                                                                                  tctGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATAtt  >  1:174081/1‑140 (MQ=255)                                                                                                                                  tctGCGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATAtt  >  1:1295832/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                        GTAAATAATTATTGTGGTCGGTGACGCTGACTCTGGCAGATCTGACCCGTTTTTACATAATCGAGAAATGCGCGCAAGCCGCAGGACATATACTGGCGGTTTGGATAATAGATCTGGAAGCCTGGACGCTCTGTGCTCCAGTCTTCCAGTACACATTCTAAACGACCAGTATCAAGGTACTCCTTGATCTGTTCATAGAGCAGATACCCGATACCAGCACCCATCAAAACGGCCTCCAGTTCAGCATCAACATCATCCAGAATGATATT  >  NC_000913/948652‑948920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑