Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,057,108	A→G	E283G (GAA→GGA)	torT →	periplasmic trimethylamine‑N‑oxide binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,057,108	0	A	G	100.0%	130.4 / NA	37	E283G (GAA→GGA)	torT	periplasmic trimethylamine‑N‑oxide binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/20);  total (17/20)

GCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGAACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCGTGAACATATTCGCCGCTCG  >  NC_000913/1056972‑1057226                                                                                                                                         |                                                                                                                       gcggcAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACt                                                                                                                     >  1:174486/1‑140 (MQ=255) gcggcAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACt                                                                                                                     >  1:36263/1‑140 (MQ=255) gcggcAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACt                                                                                                                     <  2:36263/140‑1 (MQ=255)           gggAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAg                                                                                                           >  2:944419/1‑140 (MQ=255)           gggAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAg                                                                                                           <  2:1159672/140‑1 (MQ=255)             gAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGGTGAgga                                                                                                         >  2:1092289/1‑138 (MQ=255)                   cGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCAt                                                                                                   >  1:344224/1‑140 (MQ=255)                     tAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAgg                                                                                                       <  2:817261/134‑1 (MQ=255)                     tAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAgg                                                                                                       >  1:817261/1‑134 (MQ=255)                     tAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCa                                                                                                 <  1:236668/140‑1 (MQ=255)                      gaCCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAg                                                                                                <  1:180597/139‑1 (MQ=255)                       aCCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAgg                                                                                               >  2:1148768/1‑140 (MQ=255)                            aaaaCGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAg                                                                                                           >  2:620148/1‑123 (MQ=255)                            aaaaCGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAg                                                                                                           <  1:620148/123‑1 (MQ=255)                            aaaaCGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAg                                                                                                           <  2:1231753/123‑1 (MQ=255)                            aaaaCGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAg                                                                                                           >  1:1231753/1‑123 (MQ=255)                            aaaaCGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAAtt                                                                                          >  1:769396/1‑140 (MQ=255)                            aaaaCGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAAtt                                                                                          >  1:1301555/1‑140 (MQ=255)                                  ccgcTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGTGCAGGGCATCCGGCAAATACAagg                                                                                    >  2:180597/1‑140 (MQ=255)                                  ccgcTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAggg                                                                                    <  1:944419/140‑1 (MQ=255)                                   cgcTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAgggg                                                                                   <  1:1148768/140‑1 (MQ=255)                                           gtggtgTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGt                                                                           <  2:344224/140‑1 (MQ=255)                                             ggtgTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGttt                                                                         <  2:349433/140‑1 (MQ=255)                                             ggtgTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGttt                                                                         <  1:696222/140‑1 (MQ=255)                                             ggtgTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGttt                                                                         <  2:769396/140‑1 (MQ=255)                                                 tCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGa                                                                     <  1:700314/140‑1 (MQ=255)                                                  cGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGAt                                                                    <  2:228800/140‑1 (MQ=255)                                                              cacaTCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTTAGCCAGCCCTCAGGGAAATAAAGGGGGAAACGGGTTTTTGTAAAGTGAGcccc                                                        >  2:310945/1‑139 (MQ=255)                                                              cacaTCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCa                                                        <  1:310945/140‑1 (MQ=255)                                                                caTCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCAcc                                                      <  2:1301555/140‑1 (MQ=255)                                                                                                 gTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGGAATTAAAGGGGGAAACGGGTTTTTTTAAAGTCAGCCCCCCCATTTTTGTTTTGACGCCGAAAAAAGCCGAccc                     >  2:135981/1‑139 (MQ=255)                                                                                                              ccAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTTAGGAGGGCATCAGGCAATTACAAGGGGGAACGGGTTTTGATAATGTCAGCCCCCCCATTTTTGTTTTGACGCCGAAAAAAACCCACCCTGGACAAATTtgc        >  2:1035043/1‑140 (MQ=255)                                                                                                              ccAGCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGGGCAGGGCCTCAGGCAATTACAGGGGGAATCGGGTTTTGATAATTTCAGCCCCCCCCTTTTTGTTTTTACGCCCAAAAAAGCCGACCGGGGACATAtttcc        >  2:288409/1‑137 (MQ=255)                                                                                                                 gCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCGTGAACATATTcgccgc     <  2:174486/140‑1 (MQ=255)                                                                                                                 gCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCGTGAACATATTcgccgc     <  1:785691/140‑1 (MQ=255)                                                                                                                 gCGATCAAATGGTCTGGCAGGGGGGACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCGTGAACATATTcgccgc     <  1:1085447/140‑1 (MQ=255)                                                                                                                    aTCAAATGGTCTGGCAGGGGGGACTGGCGGTTTAGCAGGCCCTCAGGCAATTACAGGGGGAATCGGGTTTTTGTAATGTCAGCCCCCCCATTTTTGTTTTGACGCCGAAAAAAGCCGACCGTGAAAATATTtgccccccc  >  2:1009348/1‑135 (MQ=255)                                                                                                                                         |                                                                                                                       GCGGCAATGGGGGAAGGGCGTAACCTGAAAACGCCGCTTACCGTGGTGTCGTTTTATCTTTCACATCAGGTGTATCGCGGGCTGAAGCGGGGAAGAGTGATTATGGCTGCCAGCGATCAAATGGTCTGGCAGGGGGAACTGGCGGTTGAGCAGGCCATCAGGCAATTACAGGGGCAATCGGTTTCTGATAATGTCAGCCCACCGATTTTAGTTCTGACGCCGAAAAATGCCGACCGTGAACATATTCGCCGCTCG  >  NC_000913/1056972‑1057226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑