Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,111,354	T→C	G164G (GGT→GGC)	opgH →	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,111,354	0	T	C	100.0%	265.8 / NA	74	G164G (GGT→GGC)	opgH	osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/35);  total (39/35)

TTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGTTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGTCCGCC  >  NC_000913/1111217‑1111483                                                                                                                                          |                                                                                                                                  ttGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTg                                                                                                                                 >  2:1132438/1‑140 (MQ=255)   gACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTggg                                                                                                                               >  2:193941/1‑140 (MQ=255)    aCCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGgc                                                                                                                              >  2:991538/1‑140 (MQ=255)     ccAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGgcg                                                                                                                             <  1:1066070/140‑1 (MQ=255)          gaagaGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGAt                                                                                                                        <  1:867209/140‑1 (MQ=255)            agagCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTa                                                                                                                      <  2:915775/140‑1 (MQ=255)             gagCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTaa                                                                                                                     <  1:1325036/140‑1 (MQ=255)              agCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAAt                                                                                                                    >  2:665212/1‑140 (MQ=255)              agCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAAt                                                                                                                    <  1:391792/140‑1 (MQ=255)                 aGGAGAGCGAGCAAAAGTGGCGTCCCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCt                                                                                                                 <  1:1041074/140‑1 (MQ=255)                 aGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCt                                                                                                                 <  2:745007/140‑1 (MQ=255)                 aGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCt                                                                                                                 <  1:1132438/140‑1 (MQ=255)                 aGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCt                                                                                                                 <  2:1127103/140‑1 (MQ=255)                   gagaGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGgcg                                                                                                                             >  2:1321579/1‑126 (MQ=255)                   gagaGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGgcg                                                                                                                             <  1:1321579/126‑1 (MQ=255)                   gagaGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTAt                                                                                                               >  1:876761/1‑140 (MQ=255)                     gagcgagcAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATgg                                                                                                             >  2:373850/1‑140 (MQ=255)                           gcAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATAtgg                                                                                                       >  2:394009/1‑140 (MQ=255)                                aGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATAtggttggt                                                                                                  >  1:1120597/1‑140 (MQ=255)                                  tGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCa                                                                                                <  1:56382/140‑1 (MQ=255)                                    gCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAgg                                                                                              >  1:749781/1‑140 (MQ=255)                                     cGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATAtggttgg                                                                                                   <  2:631526/134‑1 (MQ=255)                                     cGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATAtggttgg                                                                                                   >  1:631526/1‑134 (MQ=255)                                         ccGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTtg                                                                                         >  2:742876/1‑140 (MQ=255)                                              ggTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCATTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGt                                                                                    <  2:755306/140‑1 (MQ=255)                                                   cATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGtt                                                                                   <  2:723644/136‑1 (MQ=255)                                                   cATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGtt                                                                                   >  1:723644/1‑136 (MQ=255)                                                       cgccgTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTAt                                                                           <  1:394009/140‑1 (MQ=255)                                                              aCATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCtt                                                                    <  1:979466/140‑1 (MQ=255)                                                              aCATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCtt                                                                    <  1:373850/140‑1 (MQ=255)                                                                aTTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCt                                                                  <  2:749781/140‑1 (MQ=255)                                                                    tGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCt                                                              >  2:934488/1‑140 (MQ=255)                                                                    tGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCt                                                              >  2:1415917/1‑140 (MQ=255)                                                                              gACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTtat                                                           <  2:792614/133‑1 (MQ=255)                                                                              gACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATAtgctgc                                                    >  2:1071030/1‑140 (MQ=255)                                                                              gACGCTCGCGCAAACTGTCGTCGCGACCTGGCATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTtat                                                           >  1:792614/1‑133 (MQ=255)                                                                               aCGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCa                                                   <  2:119093/140‑1 (MQ=255)                                                                               aCGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCa                                                   <  2:876761/140‑1 (MQ=255)                                                                                cGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCaa                                                  >  1:1167230/1‑140 (MQ=255)                                                                                    cgcgCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAAc                                                <  1:573789/138‑1 (MQ=255)                                                                                    cgcgCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAAc                                                >  2:573789/1‑138 (MQ=255)                                                                                    cgcgCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCg                                              <  1:665212/140‑1 (MQ=255)                                                                                    cgcgCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCg                                              >  2:1092574/1‑140 (MQ=255)                                                                                     gcgcAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCgg                                             <  1:1029890/140‑1 (MQ=255)                                                                                      cgcAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCtt                                                             >  2:624628/1‑123 (MQ=255)                                                                                      cgcAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCtt                                                             <  1:624628/123‑1 (MQ=255)                                                                                      cgcAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGt                                            >  2:278444/1‑140 (MQ=255)                                                                                      cgcAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGt                                            >  1:669861/1‑140 (MQ=255)                                                                                       gcAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTa                                           <  1:660737/140‑1 (MQ=255)                                                                                       gcAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTa                                           <  1:1092574/140‑1 (MQ=255)                                                                                                    gcgACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCtt                              <  1:1071030/140‑1 (MQ=255)                                                                                                      gACCTGGTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTg                            <  1:193941/140‑1 (MQ=255)                                                                                                           ggTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTa                       <  1:934488/140‑1 (MQ=255)                                                                                                            gTATATGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTAc                      >  1:1148540/1‑140 (MQ=255)                                                                                                             tataTGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTAct                     <  1:991538/140‑1 (MQ=255)                                                                                                               tatGAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTActgt                   <  1:278444/140‑1 (MQ=255)                                                                                                                  gAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTg                            >  2:1005970/1‑128 (MQ=255)                                                                                                                  gAAGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTg                            <  1:1005970/128‑1 (MQ=255)                                                                                                                    aGACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTActgttctgt              >  2:181661/1‑140 (MQ=255)                                                                                                                     gACCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTActgttctgtt             >  1:279997/1‑140 (MQ=255)                                                                                                                      aCCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAACCCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTg            >  2:818022/1‑140 (MQ=255)                                                                                                                      aCCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTg            >  1:212030/1‑140 (MQ=255)                                                                                                                      aCCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTg            >  2:204754/1‑140 (MQ=255)                                                                                                                      aCCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTg            >  1:1268612/1‑140 (MQ=255)                                                                                                                      aCCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTg            >  1:1390597/1‑140 (MQ=255)                                                                                                                      aCCATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTg            >  1:483945/1‑140 (MQ=255)                                                                                                                       ccATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTgg           >  2:101533/1‑140 (MQ=255)                                                                                                                       ccATTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGCCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTgg           >  2:732032/1‑140 (MQ=255)                                                                                                                         aTTCTTCCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGgt         >  2:539196/1‑140 (MQ=255)                                                                                                                          ttcttcCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGgtg        >  1:828357/1‑140 (MQ=255)                                                                                                                           tcttcCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGgtgt       >  1:553578/1‑140 (MQ=255)                                                                                                                           tcttcCTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGgtgt       >  1:1405070/1‑140 (MQ=255)                                                                                                                                cTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGTccgcc  <  2:1405070/140‑1 (MQ=255)                                                                                                                                cTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGTccgcc  <  1:1191971/140‑1 (MQ=255)                                                                                                                                cTTATCAGGGCTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGTccgcc  <  2:1120597/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  TTGACCAAAGAAGAGCAGGAGAGCGAGCAAAAGTGGCGTACCGTCGGTACCATCCGCCGTTACATTCTGTTGATCCTGACGCTCGCGCAAACTGTCGTCGCGACCTGGTATATGAAGACCATTCTTCCTTATCAGGGTTGGGCGCTGATTAATCCTATGGATATGGTTGGTCAGGATTTGTGGGTTTCCTTTATGCAGCTTCTGCCTTATATGCTGCAAACCGGTATCCTGATCCTCTTTGCGGTACTGTTCTGTTGGGTGTCCGCC  >  NC_000913/1111217‑1111483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑