Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	136,437	T→G	T10P (ACG→CCG)	speE ←	spermidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	136,437	0	T	G	100.0%	349.5 / NA	98	T10P (ACG→CCG)	speE	spermidine synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (49/49);  total (49/49)

TACTACGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGA  >  NC_000913/136306‑136569                                                                                                                                    |                                                                                                                                     tactacGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGcc                                                                                                                              <  1:66597/140‑1 (MQ=255)  actacGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCa                                                                                                                             <  2:165325/140‑1 (MQ=255)      cGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGt                                                                                                                         >  2:960130/1‑140 (MQ=255)      cGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTACATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGt                                                                                                                         >  2:464334/1‑140 (MQ=255)       gCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGtt                                                                                                                        >  1:585391/1‑140 (MQ=255)        ccATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGttt                                                                                                                       >  2:802031/1‑140 (MQ=255)         cATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGtttt                                                                                                                      >  1:760958/1‑140 (MQ=255)           tCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGtttttt                                                                                                                    >  2:485692/1‑140 (MQ=255)           tCCAGCGCCATTACGCGACCAAATGCAGCGTTCTC‑AAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGttttttt                                                                                                                   >  2:414889/1‑140 (MQ=255)             cAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGtttttttt                                                                                                                  <  1:1168384/140‑1 (MQ=255)             cAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGtttttttt                                                                                                                  <  2:585391/140‑1 (MQ=255)               gcgcCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCg                                                                                                                <  2:1063349/140‑1 (MQ=255)                cgcCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCgg                                                                                                               >  2:450696/1‑140 (MQ=255)                cgcCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCgg                                                                                                               >  1:978391/1‑140 (MQ=255)                     ttACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCAt                                                                                                           >  1:684698/1‑139 (MQ=255)                     ttACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCAt                                                                                                           <  2:684698/139‑1 (MQ=255)                       aCGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATggg                                                                                                        <  1:343452/140‑1 (MQ=255)                           gACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGa                                                                                                    <  1:1067593/140‑1 (MQ=255)                            aCCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTAATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGAt                                                                                                   <  2:143197/140‑1 (MQ=255)                             ccAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCAt                                                                                                           <  1:967091/131‑1 (MQ=255)                             ccAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCAt                                                                                                           >  2:967091/1‑131 (MQ=255)                               aaaTGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATAcc                                                                                                >  1:252998/1‑140 (MQ=255)                                aaTGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATAcct                                                                                               <  1:803924/140‑1 (MQ=255)                                aaTGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATAcct                                                                                               <  1:215498/140‑1 (MQ=255)                                   gCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATAcctcct                                                                                            <  2:894266/140‑1 (MQ=255)                                    cAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCtt                                                                                           <  2:252998/140‑1 (MQ=255)                                       cGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATa                                                                                                  >  2:611622/1‑130 (MQ=255)                                       cGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATa                                                                                                  <  1:611622/130‑1 (MQ=255)                                       cGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGt                                                                                        >  1:174997/1‑140 (MQ=255)                                           ctcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATAcct                                                                                               <  2:1390324/129‑1 (MQ=255)                                           ctcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATAcct                                                                                               >  1:1390324/1‑129 (MQ=255)                                            tcAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAaca                                                                                   <  1:450696/140‑1 (MQ=255)                                              aaaaaTGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACAcc                                                                                 <  2:1322848/140‑1 (MQ=255)                                                   tGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATAcctcct                                                                                            <  1:743039/124‑1 (MQ=255)                                                   tGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATAcctcct                                                                                            >  2:743039/1‑124 (MQ=255)                                                           tCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTGGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAggg                                                                    <  1:1021260/140‑1 (MQ=255)                                                           tCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAggg                                                                    <  2:760958/140‑1 (MQ=255)                                                             cTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcg                                                                  <  1:943464/140‑1 (MQ=255)                                                             cTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcg                                                                  <  2:200592/140‑1 (MQ=255)                                                             cTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcg                                                                  <  1:423896/140‑1 (MQ=255)                                                             cTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcg                                                                  <  1:414889/140‑1 (MQ=255)                                                               ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCa                                                                >  2:725783/1‑140 (MQ=255)                                                               ggTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCa                                                                >  2:692786/1‑140 (MQ=255)                                                                  gATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTGGCGGATTATGCCACTGTTTTTTTTTGGCCATGGGTTGATACCTCCCTTGTTAAAACCCGCAAAAAAAGGGCGCAACa                                                             >  2:1021260/1‑140 (MQ=255)                                                                   aTCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACat                                                            <  1:315249/140‑1 (MQ=255)                                                                    tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTaaaaa                                                                         >  2:524992/1‑126 (MQ=255)                                                                    tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTaaaaa                                                                         <  1:524992/126‑1 (MQ=255)                                                                    tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcg                                                                  >  1:1289614/1‑133 (MQ=255)                                                                    tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGgcg                                                                  <  2:1289614/133‑1 (MQ=255)                                                                    tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                           <  1:802031/140‑1 (MQ=255)                                                                    tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                           <  2:174997/140‑1 (MQ=255)                                                                    tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                           >  1:695907/1‑140 (MQ=255)                                                                    tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                           >  2:470638/1‑140 (MQ=255)                                                                    tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                           <  1:730663/140‑1 (MQ=255)                                                                    tCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                           >  1:192386/1‑140 (MQ=255)                                                                     cGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTaaaaaa                                                                        >  2:898064/1‑126 (MQ=255)                                                                     cGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTaaaaaa                                                                        <  1:898064/126‑1 (MQ=255)                                                                     cGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                           <  2:109295/139‑1 (MQ=255)                                                                     cGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACata                                                           >  1:109295/1‑139 (MQ=255)                                                                      ggTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACataat                                                         <  2:1069156/140‑1 (MQ=255)                                                                        tCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAg                                                       <  1:296000/140‑1 (MQ=255)                                                                          ttttCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACat                                                            >  1:443452/1‑133 (MQ=255)                                                                          ttttCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACat                                                            <  2:443452/133‑1 (MQ=255)                                                                          ttttCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCt                                                     >  1:1117178/1‑140 (MQ=255)                                                                                  aTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACAtt                                               <  2:67032/138‑1 (MQ=255)                                                                                  aTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACAtt                                               >  1:67032/1‑138 (MQ=255)                                                                                  aTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGa                                             >  2:989899/1‑140 (MQ=255)                                                                                  aTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGa                                             >  2:776342/1‑140 (MQ=255)                                                                                  aTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGa                                             >  1:392840/1‑140 (MQ=255)                                                                                  aTACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGa                                             >  1:1206486/1‑140 (MQ=255)                                                                                   tACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGAc                                            <  2:509300/140‑1 (MQ=255)                                                                                     cAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGActg                                          <  1:470638/140‑3 (MQ=255)                                                                                      aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcgc                                         >  1:1054534/1‑140 (MQ=255)                                                                                      aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcgc                                         >  1:462336/1‑140 (MQ=255)                                                                                      aGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACcgc                                         >  2:607340/1‑140 (MQ=255)                                                                                        aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCgg                                       >  1:158899/1‑140 (MQ=255)                                                                                        aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCgg                                       >  1:946141/1‑140 (MQ=255)                                                                                        aaCGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCgg                                       >  1:363805/1‑140 (MQ=255)                                                                                           gTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATg                                    >  2:857286/1‑140 (MQ=255)                                                                                           gTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATg                                    >  2:614113/1‑140 (MQ=255)                                                                                            ttATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGc                                   <  1:607340/140‑1 (MQ=255)                                                                                            ttATCTACCGC‑AAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                  <  1:1199196/140‑1 (MQ=255)                                                                                             tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                  <  2:192386/140‑1 (MQ=255)                                                                                             tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                  <  1:857286/140‑1 (MQ=255)                                                                                             tATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                  <  1:776342/140‑1 (MQ=255)                                                                                               tCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACg                                <  2:946141/140‑1 (MQ=255)                                                                                                  aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                             <  1:692786/140‑1 (MQ=255)                                                                                                  aCCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                             >  2:214566/1‑140 (MQ=255)                                                                                                    cGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                  <  1:926903/133‑1 (MQ=255)                                                                                                    cGCAAAGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCa                                  >  2:926903/1‑133 (MQ=255)                                                                                                       aaaGTACTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAatat                        >  2:1229602/1‑140 (MQ=255)                                                                                                            aCTGCCCAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTa                   <  2:1054534/140‑1 (MQ=255)                                                                                                                cccAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                             <  2:1082833/126‑1 (MQ=255)                                                                                                                cccAAACTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTc                             >  1:1082833/1‑126 (MQ=255)                                                                                                                   aaaCTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAg            >  1:1112710/1‑140 (MQ=255)                                                                                                                     aCTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGt          >  2:380208/1‑140 (MQ=255)                                                                                                                     aCTGGTCGTGTAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGt          >  2:23652/1‑140 (MQ=255)                                                                                                                             tgtAGCGGTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGa  >  2:393235/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                     TACTACGCCATCCAGCGCCATTACGCGACCAAATGCAGCGTTCTCAAAAATGATCAGATCCTGGTGATCGGTCTTTTCATGATACAGAACGTTATCTACCGCAAAGTACTGCCCAAACTGGTCGTGTAGCGTTTCATGCCACTGTTTTTTTTCGGCCATGGGTTGATACCTCCTTTGTTAACACCCGTAAAAAAAGGGCGCAACATAATAGCTAACATTGACCGCGGATGCACGGTCAATATTTCTACAAAAAGGTATCAGGGA  >  NC_000913/136306‑136569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑