Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,254,499	A→G	V485A (GTC→GCC)	ycgV ←	putative autotransporter adhesin YcgV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,254,499	0	A	G	100.0%	300.9 / NA	85	V485A (GTC→GCC)	ycgV	putative autotransporter adhesin YcgV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/44);  total (41/44)

GTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCAT  >  NC_000913/1254367‑1254635                                                                                                                                     |                                                                                                                                         gTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGtt                                                                                                                                   >  2:461094/1‑140 (MQ=255) gTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGtt                                                                                                                                   <  1:334996/140‑1 (MQ=255)  tACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTa                                                                                                                                  >  1:836270/1‑140 (MQ=255)    cTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGTCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTAtt                                                                                                                                >  2:1021042/1‑140 (MQ=255)    cTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCTGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTAtt                                                                                                                                >  2:1177579/1‑140 (MQ=255)    cTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTAtt                                                                                                                                >  2:782314/1‑140 (MQ=255)    cTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTAtt                                                                                                                                >  2:128179/1‑140 (MQ=255)    cTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTAtt                                                                                                                                >  2:277888/1‑140 (MQ=255)    cTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTAtt                                                                                                                                >  1:429401/1‑140 (MQ=255)    cTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTAtt                                                                                                                                >  1:35057/1‑140 (MQ=255)      gTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCa                                                                                                                              <  2:984155/140‑1 (MQ=255)         aCCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTAGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTg                                                                                                                           >  2:471324/1‑140 (MQ=255)          ccGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGc                                                                                                                          >  1:454543/1‑140 (MQ=255)           cGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCa                                                                                                                         <  2:972959/140‑1 (MQ=255)           cGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCa                                                                                                                         >  1:686022/1‑140 (MQ=255)            gCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCaa                                                                                                                        >  1:210203/1‑140 (MQ=255)            gCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCaa                                                                                                                        >  2:171016/1‑140 (MQ=255)               cAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGt                                                                                                                     >  1:850079/1‑140 (MQ=255)               cAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGt                                                                                                                     >  1:748167/1‑140 (MQ=255)                     ctctACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGtt                                                                                                               >  2:408389/1‑140 (MQ=255)                          cGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCg                                                                                                          <  1:1007300/140‑1 (MQ=255)                            tttAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGcc                                                                                                        >  1:872145/1‑140 (MQ=255)                             ttAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCa                                                                                                       >  1:865178/1‑140 (MQ=255)                               aaTGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCAtt                                                                                                     <  1:171016/140‑1 (MQ=255)                                aTGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTg                                                                                                    >  2:638038/1‑140 (MQ=255)                                  gtgGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGAc                                                                                                  >  2:951209/1‑140 (MQ=255)                                   tgGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACa                                                                                                 <  2:410231/140‑1 (MQ=255)                                    ggCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACAt                                                                                                >  2:400662/1‑140 (MQ=255)                                     gCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACAtt                                                                                               >  2:561334/1‑140 (MQ=255)                                      cAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTa                                                                                              >  2:588659/1‑140 (MQ=255)                                      cAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTa                                                                                              >  2:1287658/1‑140 (MQ=255)                                      cAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTa                                                                                              >  2:1217405/1‑140 (MQ=255)                                           gtgCCGACAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCt                                                                                         <  2:366188/140‑1 (MQ=255)                                            tgCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTg                                                                                        <  1:128179/140‑1 (MQ=255)                                               cGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTgagg                                                                                     >  1:98385/1‑140 (MQ=255)                                                 gCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTgaggga                                                                                   <  1:951209/140‑1 (MQ=255)                                                  cAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTgagggag                                                                                  >  2:111259/1‑140 (MQ=255)                                                     ttGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATc                                                                                            <  2:733812/127‑1 (MQ=255)                                                     ttGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATc                                                                                            >  1:733812/1‑127 (MQ=255)                                                      tGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAAc                                                                              <  2:748167/140‑1 (MQ=255)                                                      tGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAAc                                                                              <  1:782314/140‑1 (MQ=255)                                                       gACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAAcc                                                                             <  1:796165/140‑1 (MQ=255)                                                            gTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTcc                                                                        >  1:103933/1‑140 (MQ=255)                                                             tggctggcAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCa                                                                       >  2:760155/1‑140 (MQ=255)                                                               gctggcAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCaaa                                                                     <  2:764542/140‑1 (MQ=255)                                                                ctggcAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAAc                                                                    <  1:111259/140‑1 (MQ=255)                                                                ctggcAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAAc                                                                    <  2:103933/140‑1 (MQ=255)                                                                   gcAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGa                                                                 >  1:641220/1‑140 (MQ=255)                                                                    cAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGAc                                                                <  1:588659/140‑1 (MQ=255)                                                                      aaaTCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGAccc                                                              <  2:686022/140‑1 (MQ=255)                                                                       aaTCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCa                                                             <  2:499997/140‑1 (MQ=255)                                                                       aaTCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCa                                                             <  2:98385/140‑1 (MQ=255)                                                                        aTCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACTTGTCCAAACCGACCCAg                                                            <  2:434199/140‑1 (MQ=255)                                                                        aTCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAg                                                            <  2:784227/140‑1 (MQ=255)                                                                        aTCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAg                                                            <  1:471324/140‑1 (MQ=255)                                                                            aCAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTg                                                        <  2:641220/140‑1 (MQ=255)                                                                            aCAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTg                                                        <  1:1217405/140‑1 (MQ=255)                                                                             cAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGc                                                       >  2:662650/1‑140 (MQ=255)                                                                             cAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGc                                                       <  2:468814/140‑1 (MQ=255)                                                                             cAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGc                                                       <  1:760155/140‑1 (MQ=255)                                                                                 tGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCatat                                                   <  2:429401/140‑1 (MQ=255)                                                                                 tGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCAAGGGTGCatat                                                   <  2:865178/140‑1 (MQ=255)                                                                                          cAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAgattga                                          >  2:1105657/1‑140 (MQ=255)                                                                                          cAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAgattga                                          >  1:423226/1‑140 (MQ=255)                                                                                             cgcCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTa                                       >  1:37344/1‑140 (MQ=255)                                                                                               ccAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGc                                     <  1:561334/140‑1 (MQ=255)                                                                                                ctgCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGcc                                    <  1:1177579/138‑1 (MQ=255)                                                                                                cAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGcc                                    <  2:37344/140‑1 (MQ=255)                                                                                                cAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGcc                                    <  2:210203/140‑1 (MQ=255)                                                                                                  gCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAgattga                                          >  2:668627/1‑132 (MQ=255)                                                                                                  gCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAgattga                                          <  1:668627/132‑1 (MQ=255)                                                                                                  gCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCa                                  <  2:454543/140‑1 (MQ=255)                                                                                                            aTTAGAATTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGaaa                        >  1:1014197/1‑140 (MQ=255)                                                                                                                 aaTTACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACg                   <  1:1167822/140‑1 (MQ=255)                                                                                                                    tACTTGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTa                <  2:1014197/140‑1 (MQ=255)                                                                                                                       ttGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTCGGAAAGAACGCTACCg             <  2:35057/140‑1 (MQ=255)                                                                                                                        tGTTACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGt            <  2:872145/140‑1 (MQ=255)                                                                                                                          ttACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTg          >  2:151842/1‑140 (MQ=255)                                                                                                                          ttACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTg          >  2:149478/1‑140 (MQ=255)                                                                                                                           tACGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGa         >  2:1084263/1‑140 (MQ=255)                                                                                                                            aCGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGAt        <  2:850079/140‑1 (MQ=255)                                                                                                                             cGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATa       <  1:117404/140‑1 (MQ=255)                                                                                                                             cGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATa       <  1:149478/140‑1 (MQ=255)                                                                                                                             cGTTCCAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATa       <  1:151842/140‑1 (MQ=255)                                                                                                                                  cAGGCGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCAt  <  1:662650/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GTACTGTTACCGCTCAGGTTCTCTACGTTTAATGTGGCAAATGTGCCGGCAGTTGACCCGTGGCTGGCAAAATCGACAGTTGAATGGCTCAGCGCCAGCGTGTCGAGATTAGAATTACTTGTTACGTTCCAGACGCTGTTATTCATTGCAACGTCCAGTTTTCCGCCATTGACATTATCGCTGAGGGAGGAACCTGTCCAAACCGACCCAGGGTGCATATCCAGATTGATTAGCCCACCTTTGGAAAGAACGCTACCGTTGATAAGCAT  >  NC_000913/1254367‑1254635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑