Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,274,621	C→T	P280S (CCT→TCT)	ychO →	putative invasin YchO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,274,621	0	C	T	100.0%	293.6 / NA	82	P280S (CCT→TCT)	ychO	putative invasin YchO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/43);  total (39/43)

GATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCCCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGA  >  NC_000913/1274485‑1274757                                                                                                                                         |                                                                                                                                         gATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCtg                                                                                                                                       <  2:1029913/140‑1 (MQ=255) gATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCtg                                                                                                                                       <  2:274565/140‑1 (MQ=255) gATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCtg                                                                                                                                       <  1:916397/140‑1 (MQ=255)   tGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCtgtg                                                                                                                                     <  1:348413/140‑1 (MQ=255)              ttCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCAc                                                                                                                          <  2:345689/140‑1 (MQ=255)                   tCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGa                                                                                                                     <  1:744094/140‑1 (MQ=255)                      acacCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACgg                                                                                                                  >  1:222580/1‑140 (MQ=255)                       cacCTCAATACCATTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGAGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 <  2:567776/140‑1 (MQ=255)                       cacCTCAATACCAGTGTCGGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 <  1:568061/140‑1 (MQ=255)                       cacCTCAATACCAGTGTCAGTCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 <  1:647070/140‑1 (MQ=255)                       cacCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 <  1:610738/140‑1 (MQ=255)                       cacCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 <  2:716580/140‑1 (MQ=255)                       cacCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 <  2:368182/140‑1 (MQ=255)                        acCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGcc                                                                                                                >  2:538409/1‑140 (MQ=255)                        acCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGcc                                                                                                                <  2:222580/140‑1 (MQ=255)                          cTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGt                                                                                                                             >  2:892213/1‑125 (MQ=255)                          cTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGt                                                                                                                             <  1:892213/125‑1 (MQ=255)                             aaTACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGccc                                                                                                               <  2:714042/136‑1 (MQ=255)                             aaTACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGccc                                                                                                               >  1:714042/1‑136 (MQ=255)                             aaTACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCa                                                                                                           >  1:92969/1‑140 (MQ=255)                               tACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 <  2:230701/132‑1 (MQ=255)                               tACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 >  1:230701/1‑132 (MQ=255)                                  cAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAAc                                                                                                      <  1:1390562/140‑1 (MQ=255)                                    gtgtCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 >  2:315599/1‑127 (MQ=255)                                    gtgtCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 <  1:315599/127‑1 (MQ=255)                                    gtgtCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATaaa                                                                                                       >  1:397454/1‑137 (MQ=255)                                    gtgtCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATaaa                                                                                                       <  2:397454/137‑1 (MQ=255)                                    gtgtCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAg                                                                                                    >  2:530270/1‑140 (MQ=255)                                    gtgtCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAg                                                                                                    >  2:579086/1‑140 (MQ=255)                                    gtgtCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAATCACTGTGACGGCCCAGCATAAACAg                                                                                                    >  1:559190/1‑140 (MQ=255)                                     tgtCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATT‑AATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAAc                                                                                                      >  2:915744/1‑136 (MQ=255)                                     tgtCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATT‑AATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAAc                                                                                                      <  1:915744/136‑1 (MQ=255)                                       tCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 >  2:625448/1‑124 (MQ=255)                                       tCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGc                                                                                                                 <  1:625448/124‑1 (MQ=255)                                       tCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGt                                                                                                 >  1:601980/1‑140 (MQ=255)                                           ccTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGaaa                                                                                             >  1:1363047/1‑140 (MQ=255)                                               gAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATaaa                                                                                                       <  1:237754/126‑1 (MQ=255)                                               gAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATaaa                                                                                                       >  2:237754/1‑126 (MQ=255)                                               gAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTgg                                                                                         >  1:861698/1‑140 (MQ=255)                                                aaCAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATaaa                                                                                                       >  2:859088/1‑125 (MQ=255)                                                aaCAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATaaa                                                                                                       <  1:859088/125‑1 (MQ=255)                                                     tATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGaaaa                                                                                   >  2:1364759/1‑140 (MQ=255)                                                     tATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCATTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGaaaa                                                                                   >  2:475252/1‑140 (MQ=255)                                                           ggTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCaaaa                                                                             <  2:1361742/140‑1 (MQ=255)                                                           ggTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAATCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCaaaa                                                                             <  2:559190/140‑1 (MQ=255)                                                                 cGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCt                                                                       >  1:1177410/1‑140 (MQ=255)                                                                       gATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCt                                                                 <  2:601980/140‑1 (MQ=255)                                                                       gATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCt                                                                 <  1:672071/140‑1 (MQ=255)                                                                          ttGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGaa                                                              <  1:475252/140‑1 (MQ=255)                                                                             tttAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCt                                                           >  2:487264/1‑140 (MQ=255)                                                                                   tctGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTa                                                     <  2:861698/140‑1 (MQ=255)                                                                                     tGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTAcc                                                   >  2:331259/1‑140 (MQ=255)                                                                                      ggTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  <  1:530270/140‑1 (MQ=255)                                                                                      ggTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  <  1:920282/140‑1 (MQ=255)                                                                                      ggTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  <  1:331259/140‑1 (MQ=255)                                                                                      ggTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  <  1:487264/140‑1 (MQ=255)                                                                                       gTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAGGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  >  2:611244/1‑139 (MQ=255)                                                                                       gTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAGGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  <  1:611244/139‑1 (MQ=255)                                                                                       gTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  <  1:172115/139‑1 (MQ=255)                                                                                       gTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  >  2:172115/1‑139 (MQ=255)                                                                                       gTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGc                                                 >  1:226739/1‑140 (MQ=255)                                                                                       gTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGc                                                 <  2:1363047/140‑1 (MQ=255)                                                                                        tACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCt                                                <  1:14937/140‑1 (MQ=255)                                                                                          cGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCttt                                              >  2:64210/1‑140 (MQ=255)                                                                                             gTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  <  2:1380310/133‑1 (MQ=255)                                                                                             gTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  >  1:1380310/1‑133 (MQ=255)                                                                                             gTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGgt                                           >  2:318073/1‑140 (MQ=255)                                                                                               tATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGgtgt                                         >  2:1189411/1‑140 (MQ=255)                                                                                                aTCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  >  1:865819/1‑130 (MQ=255)                                                                                                aTCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  <  2:865819/130‑1 (MQ=255)                                                                                                 tCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  >  2:974678/1‑129 (MQ=255)                                                                                                 tCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCg                                                  <  1:974678/129‑1 (MQ=255)                                                                                                      aTCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTc                                  >  2:54309/1‑140 (MQ=255)                                                                                                      aTCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTc                                  >  2:750384/1‑140 (MQ=255)                                                                                                       tCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCa                                 >  2:676295/1‑140 (MQ=255)                                                                                                       tCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCa                                 >  2:166500/1‑140 (MQ=255)                                                                                                       tCCCGTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCa                                 >  1:1229713/1‑140 (MQ=255)                                                                                                           gTCGCGTTGAGTCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCaaaaa                             >  1:515799/1‑140 (MQ=255)                                                                                                                      tCTGGGATTAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTg                  <  1:318073/140‑1 (MQ=255)                                                                                                                              tAAATTACACCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAg          <  2:297701/140‑1 (MQ=255)                                                                                                                                     cacCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCg   <  1:64210/140‑1 (MQ=255)                                                                                                                                      acCTCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGa  >  1:258838/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         GATGCGCATGCCGTTCTATCAACACCTCAATACCAGTGTCAGCCTAGAACAGTATTTTGGTGATCGTGTTGATTTGTTTAACTCTGGTACGGGTTATCACAATCCCGTCGCGTTGAGTCTGGGATTAAATTACACCCCTGTGCCATTAGTCACTGTGACGGCCCAGCATAAACAGGGTGAAAGTGGCGAAAATCAAAATAACCTCGGGCTGAATCTTAATTACCGCTTTGGTGTACCGCTCAAAAAACAACTTTCTGCGGGCGAGGTTGCCGA  >  NC_000913/1274485‑1274757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑