Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,342,543	A→G	E208G (GAA→GGA)	pyrF →	orotidine‑5'‑phosphate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,342,543	0	A	G	100.0%	338.7 / NA	94	E208G (GAA→GGA)	pyrF	orotidine‑5'‑phosphate decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/48);  total (46/48)

TTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGAACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTCCAACAGCCGT  >  NC_000913/1342408‑1342678                                                                                                                                        |                                                                                                                                        ttGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACACCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAg                                                                                                                                     <  2:622356/140‑1 (MQ=255)    aTGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCg                                                                                                                                  <  2:441569/140‑1 (MQ=255)           ggtgtgTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCgg                                                                                                                           <  2:173947/140‑1 (MQ=255)           ggtgtgTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCgg                                                                                                                           >  1:1414714/1‑140 (MQ=255)            gtgtgtTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGTCCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGc                                                                                                                          >  1:1297508/1‑140 (MQ=255)            gtgtgtTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCGTTATGACGCCAGGACAGGCGTTGTCGGc                                                                                                                          >  2:882238/1‑140 (MQ=255)            gtgtgtTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGc                                                                                                                          <  2:1307433/140‑1 (MQ=255)            gtgtgtTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGc                                                                                                                          <  2:1414714/140‑1 (MQ=255)            gtgtgtTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGc                                                                                                                          >  2:93229/1‑140 (MQ=255)             tgtgtTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACTGGCGTTGTCGGCt                                                                                                                         <  2:1114922/140‑1 (MQ=255)             tgtgtTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCt                                                                                                                         >  1:221472/1‑140 (MQ=255)               tgtTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTgg                                                                                                                       >  1:241663/1‑140 (MQ=255)                 ttCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCGTTATGACGCCAGGACCGGCGTTGTCGGCTGgtg                                                                                                                     <  1:882238/140‑1 (MQ=255)                 ttCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTg                                                                                                                        <  2:878477/137‑1 (MQ=255)                 ttCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTg                                                                                                                        >  1:878477/1‑137 (MQ=255)                 ttCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGgtg                                                                                                                     >  2:1335676/1‑140 (MQ=255)                 ttCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGgtg                                                                                                                     >  2:256907/1‑140 (MQ=255)                 ttCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGgt                                                                                                                      >  2:917489/1‑139 (MQ=255)                 ttCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGgt                                                                                                                      <  1:917489/139‑1 (MQ=255)                  tCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGGTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGgtgt                                                                                                                    <  1:310481/140‑1 (MQ=255)                   cTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGtt                                                                                                                   <  2:221472/140‑1 (MQ=255)                    tGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTg                                                                                                                  >  1:369011/1‑140 (MQ=255)                    tGCTCAGGAAGCTATGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTg                                                                                                                  >  2:267096/1‑140 (MQ=255)                     gCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGa                                                                                                                 <  1:1339778/140‑1 (MQ=255)                     gCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGa                                                                                                                 <  2:1386884/140‑1 (MQ=255)                      cTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGAt                                                                                                                >  2:570611/1‑140 (MQ=255)                      cTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGAt                                                                                                                >  1:746739/1‑140 (MQ=255)                          ggAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATtata                                                                                                            <  2:1093893/140‑1 (MQ=255)                           gAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTatat                                                                                                           >  2:53032/1‑140 (MQ=255)                           gAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTatat                                                                                                           >  1:751973/1‑140 (MQ=255)                                tgtgCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGa                                                                                                      >  1:590117/1‑140 (MQ=255)                                tgtgCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGa                                                                                                      >  1:425348/1‑140 (MQ=255)                                tgtgCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGa                                                                                                      >  2:1092092/1‑140 (MQ=255)                                  tgCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGAtt                                                                                                    >  1:462594/1‑140 (MQ=255)                                  tgCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGAtt                                                                                                    >  1:356161/1‑140 (MQ=255)                                   gcgcTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGAtt                                                                                                               <  2:201851/128‑1 (MQ=255)                                   gcgcTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGAtt                                                                                                               >  1:201851/1‑128 (MQ=255)                                       tttAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCg                                                                                               <  2:424343/140‑1 (MQ=255)                                       tttAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCg                                                                                               <  1:256907/140‑1 (MQ=255)                                                gTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAAc                                                                                      >  1:1240566/1‑140 (MQ=255)                                                 tATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACg                                                                                     >  1:1075789/1‑140 (MQ=255)                                                    tCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCaa                                                                                  <  1:678282/140‑1 (MQ=255)                                                    tCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCaa                                                                                  <  1:498089/140‑1 (MQ=255)                                                    tCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCaa                                                                                  <  2:620177/140‑1 (MQ=255)                                                    tCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCaa                                                                                  <  1:201094/140‑1 (MQ=255)                                                     cGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAAt                                                                                 >  1:331475/1‑140 (MQ=255)                                                          aGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTa                                                                            >  1:576163/1‑140 (MQ=255)                                                             aGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGAt                                                                         <  1:1092092/140‑1 (MQ=255)                                                              gTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATc                                                                        >  1:482623/1‑140 (MQ=255)                                                                 cAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAg                                                                     >  2:50223/1‑140 (MQ=255)                                                                      tGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAg                                                                >  2:577893/1‑140 (MQ=255)                                                                               cGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGaaa                                                       <  2:356161/140‑1 (MQ=255)                                                                                 ggCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCa                                                                 >  2:1418620/1‑128 (MQ=255)                                                                                 ggCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCa                                                                 <  1:1418620/128‑1 (MQ=255)                                                                                       cGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCaa                                               <  2:576163/140‑1 (MQ=255)                                                                                       cGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCaa                                               >  2:1115843/1‑140 (MQ=255)                                                                                         tCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACg                                             <  1:93229/140‑1 (MQ=255)                                                                                              aGGGGAGTGAAGCTGGTGTCCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCctct                                        <  2:1297508/140‑1 (MQ=255)                                                                                                 ggAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTa                                     <  1:480530/140‑1 (MQ=255)                                                                                                  gAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTAc                                    >  1:758701/1‑140 (MQ=255)                                                                                                   aGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACa                                   <  2:1240566/140‑1 (MQ=255)                                                                                                   aGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACa                                   >  2:389090/1‑140 (MQ=255)                                                                                                   aGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACa                                   <  2:425348/140‑1 (MQ=255)                                                                                                     tGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGc                                 <  2:751973/140‑1 (MQ=255)                                                                                                      gAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCg                                <  1:389090/140‑1 (MQ=255)                                                                                                      gAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCg                                <  2:482623/140‑1 (MQ=255)                                                                                                      gAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCg                                <  1:657333/140‑1 (MQ=255)                                                                                                      gAAGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCg                                <  1:1115591/140‑1 (MQ=255)                                                                                                       aaGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCgg                               >  1:532898/1‑140 (MQ=255)                                                                                                       aaGCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCgg                               >  1:244228/1‑140 (MQ=255)                                                                                                         gCTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAg                             <  1:577893/140‑1 (MQ=255)                                                                                                          cTGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGt                            >  2:658560/1‑140 (MQ=255)                                                                                                           tGGTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTg                           <  2:241663/140‑1 (MQ=255)                                                                                                            ggTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGc                                            >  1:928985/1‑122 (MQ=255)                                                                                                            ggTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGc                                            <  2:928985/122‑1 (MQ=255)                                                                                                             gTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCa                         >  2:389699/1‑140 (MQ=255)                                                                                                             gTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCa                         >  2:345181/1‑140 (MQ=255)                                                                                                             gTGACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCa                         <  1:53032/140‑1 (MQ=255)                                                                                                               gACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGa                              <  1:1102979/133‑1 (MQ=255)                                                                                                               gACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGa                              >  2:1102979/1‑133 (MQ=255)                                                                                                               gACCAGCGCCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCatg                       <  1:570611/140‑1 (MQ=255)                                                                                                                    gcgcCGCATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAg                  >  1:1268310/1‑140 (MQ=255)                                                                                                                          cATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCat                        >  1:740306/1‑128 (MQ=255)                                                                                                                          cATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCat                        <  2:740306/128‑1 (MQ=255)                                                                                                                          cATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTc            >  2:716622/1‑140 (MQ=255)                                                                                                                          cATTATGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTc            >  1:915655/1‑140 (MQ=255)                                                                                                                              aTGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTCCAAc        <  1:855943/140‑1 (MQ=255)                                                                                                                               tGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTCCAACa       <  2:532898/140‑1 (MQ=255)                                                                                                                               tGACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTCCAACa       <  2:1268310/140‑1 (MQ=255)                                                                                                                               tGACGCCAGGACAGGCGTTGCCGGATGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTCCAACa       >  2:356797/1‑140 (MQ=255)                                                                                                                                gACGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCAGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTCCAACAg      <  2:675904/140‑1 (MQ=255)                                                                                                                                  cGCCAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTCCAACAGcc    >  2:454036/1‑140 (MQ=255)                                                                                                                                    ccAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTCCAACAGCCGt  <  1:345181/140‑1 (MQ=255)                                                                                                                                    ccAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTCCAACAGCCGt  <  1:389699/140‑1 (MQ=255)                                                                                                                                    ccAGGACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTCCAACAGCCGt  >  2:1016132/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TTGATGGTGTGGTGTGTTCTGCTCAGGAAGCTGTGCGCTTTAAACAGGTATTCGGTCAGGAGTTCAAACTGGTTACGCCGGGCATTCGTCCGCAGGGGAGTGAAGCTGGTGACCAGCGCCGCATTATGACGCCAGAACAGGCGTTGTCGGCTGGTGTTGATTATATGGTGATTGGTCGCCCGGTAACGCAATCGGTAGATCCAGCGCAGACGCTGAAAGCGATCAACGCCTCTTTACAGCGGAGTGCATGATGAGTGATTCCAACAGCCGT  >  NC_000913/1342408‑1342678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑