BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	154,667	T→C	T254A (ACG→GCG)	htrE ←	putative fimbrial usher protein HtrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	154,667	0	T	C	100.0%	358.4 / NA	99	T254A (ACG→GCG)	htrE	putative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (53/46); total (53/46)

Only 100 of 103 total aligned reads displayed.

GTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATGCACCT  >  NC_000913/154534‑154803

gTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGa                                                                                                                                    <  1:36869/140‑1 (MQ=255)

gTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGa                                                                                                                                    <  1:1306718/140‑1 (MQ=255)

 tGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGAc                                                                                                                                   >  1:1293253/1‑140 (MQ=255)

   aCTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTc                                                                                                                                 >  1:1356036/1‑140 (MQ=255)

   aCTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTc                                                                                                                                 <  1:894830/140‑1 (MQ=255)

   aCTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTc                                                                                                                                 >  2:881312/1‑140 (MQ=255)

   aCTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTc                                                                                                                                 >  2:531373/1‑140 (MQ=255)

        ggCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCaa                                                                                                                            >  1:1178263/1‑140 (MQ=255)

              ggTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGa                                                                                                                      >  1:266325/1‑140 (MQ=255)

                    ggCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTgag                                                                                                                >  1:845217/1‑140 (MQ=255)

                       aaCGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAAc                                                                                                             >  1:1317348/1‑140 (MQ=255)

                       aaCGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAAc                                                                                                             >  1:1154313/1‑140 (MQ=255)

                         cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGc                                                                                                           >  1:1404788/1‑140 (MQ=255)

                         cGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGc                                                                                                           >  1:1100895/1‑140 (MQ=255)

                          gCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCa                                                                                                          >  1:770137/1‑140 (MQ=255)

                          gCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCa                                                                                                          >  1:800456/1‑140 (MQ=255)

                          gCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCa                                                                                                          >  2:58203/1‑140 (MQ=255)

                          gCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCa                                                                                                          >  2:731053/1‑140 (MQ=255)

                          gCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCa                                                                                                          >  2:634431/1‑140 (MQ=255)

                           ccATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCTCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAg                                                                                                         <  1:1380985/140‑1 (MQ=255)

                           ccATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAg                                                                                                         <  1:1041862/140‑1 (MQ=255)

                           ccATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAg                                                                                                         <  1:569211/140‑1 (MQ=255)

                           ccATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAg                                                                                                         <  2:1423035/140‑1 (MQ=255)

                               gaatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAg                                                                                                     >  1:800210/1‑140 (MQ=255)

                                aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAgg                                                                                                    <  1:731483/140‑1 (MQ=255)

                                aatgaTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAgg                                                                                                    <  2:1154313/140‑1 (MQ=255)

                                    aTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGat                                                                                                >  2:1091793/1‑140 (MQ=255)

                                    aTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGat                                                                                                >  2:304345/1‑140 (MQ=255)

                                    aTAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGat                                                                                                >  1:1341465/1‑140 (MQ=255)

                                     tAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGata                                                                                               <  2:1293253/140‑1 (MQ=255)

                                         cgcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCAc                                                                                           <  2:1178263/140‑1 (MQ=255)

                                          gcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg                                                                                          <  1:290013/140‑1 (MQ=255)

                                          gcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg                                                                                          >  1:936451/1‑140 (MQ=255)

                                          gcAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg                                                                                          <  2:531074/140‑1 (MQ=255)

                                           cAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGc                                                                                         <  1:881312/140‑1 (MQ=255)

                                             aaGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTg                                                                                       >  1:214587/1‑140 (MQ=255)

                                              aGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg                                                                                          <  1:1256306/136‑1 (MQ=255)

                                              aGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACg                                                                                          >  2:1256306/1‑136 (MQ=255)

                                               gCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGaa                                                                                     <  1:855066/140‑1 (MQ=255)

                                                  ggCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACAt                                                                                  >  2:736399/1‑140 (MQ=255)

                                                   gCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGGTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATa                                                                                 >  2:251170/1‑140 (MQ=255)

                                                            ggAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCtt                                                                        <  1:736399/140‑1 (MQ=255)

                                                             gAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTa                                                                       <  2:1404788/140‑1 (MQ=255)

                                                             gAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTa                                                                       <  2:1406164/140‑1 (MQ=255)

                                                              aGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTaa                                                                      >  1:443209/1‑140 (MQ=255)

                                                              aGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTaa                                                                      >  2:705391/1‑140 (MQ=255)

                                                               ggCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTaaa                                                                     <  2:1341465/140‑1 (MQ=255)

                                                                 cAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAAt                                                                   <  1:304345/140‑1 (MQ=255)

                                                                   aCATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCa                                                                 >  2:1344968/1‑140 (MQ=255)

                                                                          gCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTAc                                                          >  2:344256/1‑140 (MQ=255)

                                                                            tGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTg                                                        <  1:1391067/140‑1 (MQ=255)

                                                                             gTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGc                                                       <  1:705391/140‑1 (MQ=255)

                                                                              tCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTaaa                                                                     >  1:1173208/1‑125 (MQ=255)

                                                                              tCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTaaa                                                                     <  2:1173208/125‑1 (MQ=255)

                                                                                   gTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAAt                                                 >  1:213974/1‑140 (MQ=255)

                                                                                    tataAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATc                                                >  1:731140/1‑140 (MQ=255)

                                                                                    tataAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATc                                                <  1:731053/140‑1 (MQ=255)

                                                                                     ataAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCg                                               >  1:67738/1‑140 (MQ=255)

                                                                                         aCGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCa                                           <  1:1335763/140‑1 (MQ=255)

                                                                                         aCGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCa                                           <  2:845217/140‑1 (MQ=255)

                                                                                         aCGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCa                                           <  2:443209/140‑1 (MQ=255)

                                                                                         aCGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCa                                           <  2:731140/140‑1 (MQ=255)

                                                                                         aCGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCa                                           <  1:251170/140‑1 (MQ=255)

                                                                                              tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAg                                      >  2:1074390/1‑140 (MQ=255)

                                                                                              tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAg                                      >  1:699927/1‑140 (MQ=255)

                                                                                              tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAg                                      >  2:1258402/1‑140 (MQ=255)

                                                                                              tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAg                                      >  1:1406874/1‑140 (MQ=255)

                                                                                              tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATCCTTAAAATCATAATTACTGCCAGAATCGGTCAt                                          >  1:690232/1‑136 (MQ=255)

                                                                                              tGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATCCTTAAAATCATAATTACTGCCAGAATCGGTCAt                                          <  2:690232/136‑1 (MQ=255)

                                                                                                ccTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGtt                                    <  1:1074390/140‑1 (MQ=255)

                                                                                                ccTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGtt                                    >  1:365435/1‑140 (MQ=255)

                                                                                                   cGGATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTa                                 <  2:214587/140‑1 (MQ=255)

                                                                                                     gATACTGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGt                               >  1:1306040/1‑140 (MQ=255)

                                                                                                          tGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGccc                          <  1:1344968/140‑1 (MQ=255)

                                                                                                          tGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGccc                          >  1:406083/1‑140 (MQ=255)

                                                                                                          tGACGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGccc                          >  1:74775/1‑140 (MQ=255)

                                                                                                            aCGGAATCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGa                        >  2:1176177/1‑140 (MQ=255)

                                                                                                                aaTCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCa                    >  2:95699/1‑140 (MQ=255)

                                                                                                                aaTCAAAGGTTTCGCCCGTCGCATAAGACTCACAAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCa                    >  2:520908/1‑140 (MQ=255)

                                                                                                                 aTCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCAc                   <  2:74775/140‑1 (MQ=255)

                                                                                                                  tCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACg                  >  2:408316/1‑140 (MQ=255)

                                                                                                                  tCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACg                  >  2:309998/1‑140 (MQ=255)

                                                                                                                  tCAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACg                  >  2:138875/1‑140 (MQ=255)

                                                                                                                   cAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGc                 <  2:1392621/140‑1 (MQ=255)

                                                                                                                   cAAAGGTTTCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGc                 <  2:800456/140‑1 (MQ=255)

                                                                                                                          ttCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGcc          >  2:208564/1‑140 (MQ=255)

                                                                                                                          ttCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGcc          >  1:327608/1‑140 (MQ=255)

                                                                                                                          ttCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGcc          <  1:58203/140‑1 (MQ=255)

                                                                                                                           tCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCa         <  2:1100895/140‑1 (MQ=255)

                                                                                                                           tCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCa         <  2:800210/140‑1 (MQ=255)

                                                                                                                           tCGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCa         <  1:520908/140‑1 (MQ=255)

                                                                                                                            cGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGc                 <  1:1361221/131‑1 (MQ=255)

                                                                                                                            cGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGc                 >  2:1361221/1‑131 (MQ=255)

                                                                                                                            cGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGc                 >  2:949306/1‑131 (MQ=255)

                                                                                                                            cGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGc                 <  1:949306/131‑1 (MQ=255)

                                                                                                                            cGCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCAt        <  2:936451/140‑1 (MQ=255)

                                                                                                                             gCCCGTCGCATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATg       <  2:699927/140‑1 (MQ=255)

                                                                                                                                cgtcgcATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATGCAc    <  1:1176177/140‑1 (MQ=255)

                                                                                                                                  tcgcATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGGATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATGCACCt  <  1:408316/140‑1 (MQ=255)

                                                                                                                                  tcgcATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATGCACCt  <  1:138875/140‑1 (MQ=255)

GTGACTTTGGCGTTGGTATTGGCAACGCCATGAATGATAGGCGCAAAGCTGGCTAAAGTCGGAGGCAACATGCGGCTGTCACTGTATAAACGAATGCCTCGGATACTGACGGAATCAAAGGTTTCGCCCGTCGTATAAGACTCACCAAGAATGAGTTGAGAACGCAGCGAGGCGATATCACGCTGAACATACCGATTCTTAAAATCATAATTACTGCCAGAATCGGTCATCCAGTTGTAGTTGCCCGAGGCACGCAGTCGCCATGCACCT  >  NC_000913/154534‑154803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`